DPO3X_BACSU
ID DPO3X_BACSU Reviewed; 563 AA.
AC P09122;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 3.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=DNA polymerase III subunit gamma/tau;
DE EC=2.7.7.7;
GN Name=dnaX; Synonyms=dnaH; OrderedLocusNames=BSU00190;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / YB886 / BG214;
RX PubMed=2124672; DOI=10.1093/nar/18.23.6771;
RA Alonso J.C., Shirahige K., Ogasawara N.;
RT "Molecular cloning, genetic characterization and DNA sequence analysis of
RT the recM region of Bacillus subtilis.";
RL Nucleic Acids Res. 18:6771-6777(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA Ogasawara N., Nakai S., Yoshikawa H.;
RT "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis
RT chromosome containing the replication origin.";
RL DNA Res. 1:1-14(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP SEQUENCE REVISION TO 211 AND 533.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-422.
RC STRAIN=168;
RX PubMed=2452406; DOI=10.1093/nar/16.6.2720;
RA Struck J.C.R., Vogel D.W., Ulbrich N., Erdmann V.A.;
RT "A dnaZX-like open reading frame downstream from the Bacillus subtilis
RT scRNA gene.";
RL Nucleic Acids Res. 16:2720-2720(1988).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5.
RC STRAIN=168;
RX PubMed=2468993; DOI=10.1007/bf00427046;
RA Struck J.C.R., Hartmann R.K., Toschka H.Y., Erdmann V.A.;
RT "Transcription and processing of Bacillus subtilis small cytoplasmic RNA.";
RL Mol. Gen. Genet. 215:478-482(1989).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5.
RC STRAIN=168;
RX PubMed=1698458; DOI=10.1016/0167-4781(90)90145-r;
RA Struck J.C.R., Kretschmer-Kazemi F.R., Schroeder W., Hucho F.,
RA Toschka H.Y., Erdmann V.A.;
RT "Characterization of a 17 kDa protein gene upstream from the small
RT cytoplasmic RNA gene of Bacillus subtilis.";
RL Biochim. Biophys. Acta 1050:80-83(1990).
CC -!- FUNCTION: DNA polymerase III is a complex, multichain enzyme
CC responsible for most of the replicative synthesis in bacteria.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC -!- SUBUNIT: DNA polymerase III contains a core (composed of alpha, epsilon
CC and theta chains) that associates with a tau subunit. This core
CC dimerizes to form the POLIII' complex. PolIII' associates with the
CC gamma complex (composed of gamma, delta, delta', psi and chi chains)
CC and with the beta chain to form the complete DNA polymerase III complex
CC (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DnaX/STICHEL family. {ECO:0000305}.
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DR EMBL; X17014; CAA34877.1; -; Genomic_DNA.
DR EMBL; D26185; BAA05255.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB11795.2; -; Genomic_DNA.
DR EMBL; X06803; CAA29958.1; -; Genomic_DNA.
DR EMBL; X52144; CAA36390.1; -; Genomic_DNA.
DR PIR; I40469; I40469.
DR PIR; S13786; S13786.
DR RefSeq; NP_387900.2; NC_000964.3.
DR RefSeq; WP_003247135.1; NZ_JNCM01000024.1.
DR AlphaFoldDB; P09122; -.
DR SMR; P09122; -.
DR IntAct; P09122; 7.
DR STRING; 224308.BSU00190; -.
DR PaxDb; P09122; -.
DR PRIDE; P09122; -.
DR EnsemblBacteria; CAB11795; CAB11795; BSU_00190.
DR GeneID; 936899; -.
DR KEGG; bsu:BSU00190; -.
DR PATRIC; fig|224308.179.peg.19; -.
DR eggNOG; COG2812; Bacteria.
DR InParanoid; P09122; -.
DR OMA; EIHCKMV; -.
DR PhylomeDB; P09122; -.
DR BioCyc; BSUB:BSU00190-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0009360; C:DNA polymerase III complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006261; P:DNA-templated DNA replication; IBA:GO_Central.
DR CDD; cd18137; HLD_clamp_pol_III_gamma_tau; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR001270; ClpA/B.
DR InterPro; IPR008921; DNA_pol3_clamp-load_cplx_C.
DR InterPro; IPR022754; DNA_pol_III_gamma-3.
DR InterPro; IPR012763; DNA_pol_III_sug/sutau_N.
DR InterPro; IPR045085; HLD_clamp_pol_III_gamma_tau.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF12169; DNA_pol3_gamma3; 1.
DR PRINTS; PR00300; CLPPROTEASEA.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF48019; SSF48019; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02397; dnaX_nterm; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA replication; DNA-directed DNA polymerase;
KW Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..563
FT /note="DNA polymerase III subunit gamma/tau"
FT /id="PRO_0000105492"
FT BINDING 45..52
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 533
FT /note="E -> A (in Ref. 1; CAA34877 and 2; BAA05255)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 563 AA; 62763 MW; F5975C246622E4F5 CRC64;
MSYQALYRVF RPQRFEDVVG QEHITKTLQN ALLQKKFSHA YLFSGPRGTG KTSAAKIFAK
AVNCEHAPVD EPCNECAACK GITNGSISDV IEIDAASNNG VDEIRDIRDK VKFAPSAVTY
KVYIIDEVHM LSIGAFNALL KTLEEPPEHC IFILATTEPH KIPLTIISRC QRFDFKRITS
QAIVGRMNKI VDAEQLQVEE GSLEIIASAA DGGMRDALSL LDQAISFSGD ILKVEDALLI
TGAVSQLYIG KLAKSLHDKN VSDALETLNE LLQQGKDPAK LIEDMIFYFR DMLLYKTAPG
LEGVLEKVKV DETFRELSEQ IPAQALYEMI DILNKSHQEM KWTNHPRIFF EVAVVKICQT
SHQSAADLPE VDMLMKKIQQ LEQEVERLKT TGIKAAAESP KKEAPRVPKG GKSNYKAPVG
RIHEILKEAT RPDLDLLRNS WGKLLAHLKQ QNKVSHAALL NDSEPVAAGS AAFVLKFKYE
IHCKMVAEDN NGVRTNLEQI LESMLGKRMD LIGVPEAQWG KIREEFLEDH QQENEGSNEP
AEEDPLIAEA KKLVGADLIE IKD
