DPO3_ALKHC
ID DPO3_ALKHC Reviewed; 1433 AA.
AC Q9KA72;
DT 04-MAY-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=BH2418;
OS Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344
OS / JCM 9153 / C-125) (Bacillus halodurans).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus.
OX NCBI_TaxID=272558;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125;
RX PubMed=11058132; DOI=10.1093/nar/28.21.4317;
RA Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F.,
RA Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.;
RT "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans
RT and genomic sequence comparison with Bacillus subtilis.";
RL Nucleic Acids Res. 28:4317-4331(2000).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR EMBL; BA000004; BAB06137.1; -; Genomic_DNA.
DR PIR; B83952; B83952.
DR RefSeq; WP_010898571.1; NC_002570.2.
DR AlphaFoldDB; Q9KA72; -.
DR SMR; Q9KA72; -.
DR STRING; 272558.10175038; -.
DR EnsemblBacteria; BAB06137; BAB06137; BAB06137.
DR KEGG; bha:BH2418; -.
DR eggNOG; COG2176; Bacteria.
DR HOGENOM; CLU_003297_2_0_9; -.
DR OMA; YYAAYFT; -.
DR OrthoDB; 561611at2; -.
DR Proteomes; UP000001258; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 2.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 2.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..1433
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_0000204574"
FT DOMAIN 419..575
FT /note="Exonuclease"
SQ SEQUENCE 1433 AA; 162126 MW; B1DEF38B3612D59F CRC64;
MNEEQRVRQE RFKLLMEQLL IPEDVTANHL KDGKIEKLTI KKDERRWHFQ LSIPTVLPAS
IYELLADRLV QTFSHIAKVS FQIQYGQPDL SEQVWQSYWP LIVAKLTNIS QPLKEMLEKQ
TPRFDGKRLV IQVGNETEAI ALKRKLTEPL QQAVQSFGFP AVQLDAEVKE SKQAFEKFVE
QRKQEDHSKV VEAILEKKKL EQQVEKKMKE EKLTIGYPIK DEPVPLETIV EEERRITVQG
YIFAAETKEL RSGRTLLTFK ITDYTDSILV KMFSRDKEDI PLLQAVKKGM WVKVRGGVQN
DTFVRDLVMI ANDVNEVSGK ETKDEAPDDE KRVELHLHTA MSQMDGISSA GAYVTQASKW
GHKAIAITDH GVVQAFPEAY GASQKHGIKV IYGVEANLVD DGVPIAYNEA HIPLLDSEFV
VFDVETTGLS AVYNKIIELA AVKVKNGEII DRFERFADPH EPLTNTIIEL TGITDDMLKG
QPEVEQVLNE FHAFIGDAVL VAHNASFDMG FLNTGFQKMG LGEAKNPVID TLELGRFLYP
TLKNHRLNTL CKKFDIELVS HHRAIYDAEA TGHLLWRMVK DATERDILYH DQLNDNMGEG
NFHRQRPSHC ILLAQTQEGL KNLYKLVSMA HVEYFYRTPR IPRSQLQKHR EGILVGSGCD
KGEVFEGMMQ KTPQEVEEIA KFYDYIEVQP LANYEHLIEK ELVKSREALQ EIVANIVKLG
EQLGKPVVAT GNAHYLKEED YIYRKILIAS QGGANPLNKQ TLPQVHFRTT SEMLEAFAFL
GEEKAKEIVV TNTNHIADQI EEIHPIPDKL YTPKIEGADE EIRQMSYNRA RKIYGDPLPE
IVEARLEKEL KSIIGHGFAV IYLISHKLVK KSLDDGYLVG SRGSVGSSFV ATMTEITEVN
PLPPHYVCPN CHHSHFFNDG SVGSGYDLPD ENCPKCGTPY VKDGQDIPFE TFLGFKGDKV
PDIDLNFSGE YQPRAHNYTK ELFGESYVYR AGTIGTVAEK TAYGYVKGYQ SDHDLHFRGA
EIDRLVTGCT GVKRTTGQHP GGIIVVPDYM DIHDFCPIQF PADDRGAEWK TTHFDFHSIH
DNLLKLDILG HDDPTVIRML QDLSGIDPKT IPTDDPEVMK IFSGPDVLGV TEEQILCKTG
TLGIPEFGTR FVRQMLEETK PSTFSELVQI SGLSHGTDVW LNNANELIYN GTCELKDVIG
CRDDIMVYLI YKGLEPSLAF KIMEFVRKGK GLQPEWIEEM KKHDVPDWYI GSCLKIKYMF
PKAHAAAYVL MAVRIAYFKV HYPILYYASY FTVRADDFDL DTMVKGSAAI RAKIEEINGK
GLDASPKEKS LLTVLELALE MVERGFSFQK VDLYRSEATE FLVEGNTLIP PFNALTGVGT
NAAINIVKAR DEREFLSKED LQQRSKITKT VLENLDAHGC LEGLPESNQL SLF
