DPO3_BACCR
ID DPO3_BACCR Reviewed; 1435 AA.
AC Q819Y5;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=BC_3816;
OS Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC
OS 15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=226900;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373
RC / NCTC 2599 / NRRL B-3711;
RX PubMed=12721630; DOI=10.1038/nature01582;
RA Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V.,
RA Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M.,
RA Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G.,
RA Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C.;
RT "Genome sequence of Bacillus cereus and comparative analysis with Bacillus
RT anthracis.";
RL Nature 423:87-91(2003).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR EMBL; AE016877; AAP10739.1; -; Genomic_DNA.
DR RefSeq; NP_833538.1; NC_004722.1.
DR AlphaFoldDB; Q819Y5; -.
DR SMR; Q819Y5; -.
DR STRING; 226900.BC_3816; -.
DR EnsemblBacteria; AAP10739; AAP10739; BC_3816.
DR KEGG; bce:BC3816; -.
DR PATRIC; fig|226900.8.peg.3933; -.
DR HOGENOM; CLU_003297_2_0_9; -.
DR OMA; YYAAYFT; -.
DR Proteomes; UP000001417; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 2.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..1435
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_1000048471"
FT DOMAIN 420..576
FT /note="Exonuclease"
SQ SEQUENCE 1435 AA; 161926 MW; 88BF90F732E75644 CRC64;
MLMSLTNEQQ ERFQILLQQL QIPDDLINQY LQGGGIERLV IDKANKSWHF DLQVPRILPT
ELYELLETKL KQSFSHIART TFALETENKQ FTEEEVRAYW PLCTERITFS PMFAYLKKQL
PQVNGVKLLI NVNNELESTA LKKNVAKPVG DQYEVFGFPR FQLDTHIQQN TEEMQKFREQ
TQQEDRERVI QAMEEMAKKQ AEESSVVYEG PITLGYLIKP DEEITPMREI QDEERRKTVQ
GYVFHVETKE LRSGRTLLTL KITDYTDSIM IKMFSRDKED IPMLQSLKKG MWVKARGSVQ
NDTFVRDLVM IANDINEITG PSRKDKAPEG EKRVELHLHT PMSQMDAVTP VSKLVAQAGK
WGHEAIAVTD HAVAQSFPEA YSAGKKAGVK VIYGVEANLV NDGVPIAYNE AHRLLAEETY
VVFDVETTGL SAVYDTVIEL AAVKVKGGEI IDRFESFANP HQPLSATIIE LTGITDDMLT
DAPEVDEVFK KFEEWMGDHT LVAHNASFDM GFINVGFKKA GLEKTNNPVI DTLELARFLF
PEMKNHRLNT MCKKLDIELT QHHRAIYDTE ATGYLLVKML KDVIEKGFEY HDQLNDSMGQ
GDAYKRGRPS HMTLLATSDV GLKNLYKLVS YSHLNYFYRV PRVPRSLLKK YREGILVGTA
CDKGEVFEAM MQKAPEEVEE IAQFYDYIEV MPPEVLRHLV ERELVRDEGQ LKTIISNLVK
LGETLDKPVV ATGNVHYLDP EDAMYRKILV SSQGGANPLN RHSLPPVHFR TTDEMLECFS
FLGEDKAKEV VVTNTQKIAS LIGDVHPVKD DLYTPKIEGA DDETRDMSYK MARSIYGEEL
PEIVEARLEK ELKSIIGHGF AVIYLISHKL VKKSLVDGYL VGSRGSVGSS FVATMMEITE
VNPLPPHYVC PKCKQSEFFN DGSVGSGFDL PDKECPACNI PYVKDGHDIP FETFLGFKGD
KVPDIDLNFS GEYQPRAHNY TKVLFGEDYV YRAGTIGTVA EKTAYGYVKG YANDHNLTIR
NAEIDRLVAG CTGVKRTTGQ HPGGIIVVPD YMDIFDFSPI QYPADSIGAE WRTTHFDFHS
IHDNLLKLDI LGHDDPTVIR MLQDLSGIDP KTIPTDDPEV MKIFSGPESL GVTEEQINCK
TGTLGIPEFG TKFVRQMLEE TKPTTFSELV QISGLSHGTD VWLGNANELI YNGTCTLSEV
IGCRDDIMVY LIYQGLDPSL AFKIMESVRK GKGVPEEWEE DMKNNNVPGW YIDSCKKIKY
MFPKAHAAAY VLMAVRIAYF KVHFALLFYA AYFTVRADDF DVEAMAKGSA SIRARIDEIA
QKGLDAAPKE KSLLTVLEMT LEMCERGYSF QKVDLYRSHA TEFIIDGDTL IPPFNAVPGL
GTNAALSIVE ARKNGDFLSK EDLQQRSKVS KTIIEYLDSQ GCLGDLPDQN QLSLF
