DPO3_BACSU
ID DPO3_BACSU Reviewed; 1437 AA.
AC P13267;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1991, sequence version 2.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=DNA polymerase III PolC-type;
DE Short=PolIII;
DE EC=2.7.7.7;
GN Name=polC; Synonyms=dnaF, mutI; OrderedLocusNames=BSU16580;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC STRAIN=168 / BD541;
RX PubMed=1901559; DOI=10.1016/0378-1119(91)90100-p;
RA Hammond R.A., Barnes M.H., Mack S.L., Mitchener J.A., Brown N.C.;
RT "Bacillus subtilis DNA polymerase III: complete sequence, overexpression,
RT and characterization of the polC gene.";
RL Gene 98:29-36(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=SB19;
RX PubMed=2499883; DOI=10.1073/pnas.86.12.4421;
RA Sanjanwala B., Ganesan A.T.;
RT "DNA polymerase III gene of Bacillus subtilis.";
RL Proc. Natl. Acad. Sci. U.S.A. 86:4421-4424(1989).
RN [3]
RP SEQUENCE REVISION.
RX PubMed=1840638; DOI=10.1007/bf00260660;
RA Sanjanwala B., Ganesan A.T.;
RT "Genetic structure and domains of DNA polymerase III of Bacillus
RT subtilis.";
RL Mol. Gen. Genet. 226:467-472(1991).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-55.
RC STRAIN=SG64;
RX PubMed=7679775; DOI=10.1007/bf00277136;
RA Sanjanwala B., Ganesan A.T.;
RT "Leader region of the gene encoding DNA polymerase III of Bacillus
RT subtilis.";
RL Mol. Gen. Genet. 236:374-378(1993).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1150-1229.
RX PubMed=2515995; DOI=10.1016/0378-1119(89)90478-2;
RA Barnes M.H., Hammond R.A., Foster K.A., Mitchener J.A., Brown N.C.;
RT "The cloned polC gene of Bacillus subtilis: characterization of the azp12
RT mutation and controlled in vitro synthesis of active DNA polymerase III.";
RL Gene 85:177-186(1989).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1398-1437.
RX PubMed=8491709; DOI=10.1128/jb.175.10.2880-2887.1993;
RA Shazand K., Tucker J., Grunberg-Manago M., Rabinowitz J.C., Leighton T.;
RT "Similar organization of the nusA-infB operon in Bacillus subtilis and
RT Escherichia coli.";
RL J. Bacteriol. 175:2880-2887(1993).
RN [8]
RP MUTAGENESIS.
RX PubMed=1312503; DOI=10.1016/0378-1119(92)90601-k;
RA Barnes M.H., Hammond R.A., Kennedy C.S., Mack S.L., Brown N.C.;
RT "Localization of the exonuclease and polymerase domains of Bacillus
RT subtilis DNA polymerase III.";
RL Gene 111:43-49(1992).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- MISCELLANEOUS: Mutant azp12 has a form of DNA polymerase III resistant
CC to hydroxyphenylazopyrimidines.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000305}.
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DR EMBL; X52116; CAA36362.1; -; Genomic_DNA.
DR EMBL; M22996; AAA22666.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13531.1; -; Genomic_DNA.
DR EMBL; S55653; AAP13898.1; -; Genomic_DNA.
DR EMBL; M33543; AAA22667.1; -; Genomic_DNA.
DR EMBL; Z18631; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; F69680; F69680.
DR RefSeq; NP_389540.1; NC_000964.3.
DR RefSeq; WP_003245843.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; P13267; -.
DR SMR; P13267; -.
DR IntAct; P13267; 3.
DR STRING; 224308.BSU16580; -.
DR BindingDB; P13267; -.
DR ChEMBL; CHEMBL3897; -.
DR PaxDb; P13267; -.
DR PRIDE; P13267; -.
DR EnsemblBacteria; CAB13531; CAB13531; BSU_16580.
DR GeneID; 939620; -.
DR KEGG; bsu:BSU16580; -.
DR PATRIC; fig|224308.179.peg.1799; -.
DR eggNOG; COG2176; Bacteria.
DR InParanoid; P13267; -.
DR OMA; YYAAYFT; -.
DR PhylomeDB; P13267; -.
DR BioCyc; BSUB:BSU16580-MON; -.
DR SABIO-RK; P13267; -.
DR PRO; PR:P13267; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR Gene3D; 1.10.150.700; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 2.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 1: Evidence at protein level;
KW Antibiotic resistance; Cytoplasm; Direct protein sequencing;
KW DNA replication; DNA-directed DNA polymerase; Exonuclease; Hydrolase;
KW Nuclease; Nucleotidyltransferase; Reference proteome; Transferase.
FT CHAIN 1..1437
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_0000204573"
FT DOMAIN 421..578
FT /note="Exonuclease"
FT REGION 613..1437
FT /note="dNTP polymerization site"
FT /evidence="ECO:0000255"
FT REGION 1393..1437
FT /note="Contains the active site of the enzyme"
FT VARIANT 1175
FT /note="S -> A (in mutant AZP12)"
FT MUTAGEN 427
FT /note="E->A: Destroys both polymerase and exonuclease
FT activities."
FT /evidence="ECO:0000269|PubMed:1312503"
FT MUTAGEN 427
FT /note="E->Q: Destroys exonuclease activity; decreases
FT polymerase activity."
FT /evidence="ECO:0000269|PubMed:1312503"
FT CONFLICT 184..188
FT /note="EDQER -> KTKSW (in Ref. 2; AAA22666)"
FT /evidence="ECO:0000305"
FT CONFLICT 495..496
FT /note="EW -> VR (in Ref. 2; AAA22666)"
FT /evidence="ECO:0000305"
FT CONFLICT 829
FT /note="M -> S (in Ref. 2; AAA22666)"
FT /evidence="ECO:0000305"
FT CONFLICT 1015
FT /note="G -> E (in Ref. 2; AAA22666)"
FT /evidence="ECO:0000305"
FT CONFLICT 1190
FT /note="E -> D (in Ref. 2; AAA22666)"
FT /evidence="ECO:0000305"
FT CONFLICT 1405..1406
FT /note="LQ -> FE (in Ref. 2; AAA22666)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1437 AA; 162663 MW; 0C04FC12D08C2E74 CRC64;
MEQLSVNRRQ FQILLQQINM TDDTFMTYFE HGEIKKLTIH KASKSWHFHF QFKSLLPFQI
YDTLTTRLTQ SFAHIAKVTS SIEVQDAEVS ESIVQDYWSR CIEELQGISP PIISLLNQQK
PKLKGNKLIV KTKTDTEAAA LKNKYSSMIQ AEYRQFGFPD LQLDAEIFVS EQEVQKFREQ
KLAEDQERAM QALIEMEKKD KESDEDQAPS GPLVIGYQIK DNEEIRTLDS IMDEERRITV
QGYVFDVETR ELKSGRTLCI FKITDYTNSI LIKMFAREKE DAALMKSLKK GMWVKARGSI
QNDTFVRDLV MIANDVNEIK AKTREDSAPE GEKRVELHLH SPMSQMDAVT GIGKLVEQAK
KWGHEAIALT DHAVVQSFPD AYSAAKKHGI KMIYGMEANL VDDGVPIAYN AAHRLLEEET
YVVFDVETTG LSAVYDTIIE LAAVKVKGGE IIDKFEAFAN PHRPLSATII ELTGITDDML
QDAPDVVDVI RDFREWIGDD ILVAHNASFD MGFLNVAYKK LLEVEKAKNP VIDTLELGRF
LYPEFKNHRL NTLCKKFDIE LTQHHRAIYD TEATAYLLLK MLKDAAEKGI QYHDELNENM
GQSNAYQRSR PYHATLLAVN STGLKNLFKL VSLSHIHYFY RVPRIPRSQL EKYREGLLIG
SACDRGEVFE GMMQKSPEEV EDIASFYDYL EVQPPEVYRH LLELELVRDE KALKEIIANI
TKLGEKLNKP VVATGNVHYL NDEDKIYRKI LISSQGGANP LNRHELPKVH FRTTDEMLEA
FSFLGEEKAK EIVVTNTQKV ASLVDDIKPI KDDLYTPKIE GADEEIREMS YQRARSIYGE
ELPEIVEARI EKELKSIIGH GFAVIYLISH KLVKRSLDDG YLVGSRGSVG SSLVATLTEI
TEVNPLPPHY VCPECQHSEF FNDGSVGSGF DLPDKTCPHC GTPLKKDGHD IPFETFLGFK
GDKVPDIDLN FSGEYQPQAH NYTKVLFGED NVYRAGTIGT VAEKTAYGYV KGYAGDNNLH
MRGAEIDRLV QGCTGVKRTT GQHPGGIIVV PDYMDIYDFS PIQFPADATG SEWKTTHFDF
HSIHDNLLKL DILGHDDPTV IRMLQDLSGI DPKTIPTDDP EVMKIFQGTE SLGVTEEQIG
CKTGTLGIPE FGTRFVRQML EDTKPTTFSE LVQISGLSHG TDVWLGNAQE LIHNNICELS
EVIGCRDDIM VYLIYQGLEP SLAFKIMEFV RKGKGLTPEW EEEMKNNNVP DWYIDSCKKI
KYMFPKAHAA AYVLMAVRIA YFKVHHALLY YAAYFTVRAD DFDIDTMIKG STAIRAVMED
INAKGLDASP KEKNLLTVLE LALEMCERGY SFQKVDLYRS SATEFIIDGN SLIPPFNSIP
GLGTNAALNI VKAREEGEFL SKEDLQKRGK VSKTILEYLD RHGCLESLPD QNQLSLF
