DPO3_CALS4
ID DPO3_CALS4 Reviewed; 1401 AA.
AC Q8RA32;
DT 25-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=TTE1398;
OS Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM
OS 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis).
OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacteraceae; Caldanaerobacter.
OX NCBI_TaxID=273068;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15242 / JCM 11007 / NBRC 100824 / MB4;
RX PubMed=11997336; DOI=10.1101/gr.219302;
RA Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y.,
RA Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R.,
RA Wang J., Yu J., Yang H.;
RT "A complete sequence of the T. tengcongensis genome.";
RL Genome Res. 12:689-700(2002).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR EMBL; AE008691; AAM24620.1; -; Genomic_DNA.
DR RefSeq; WP_011025683.1; NC_003869.1.
DR AlphaFoldDB; Q8RA32; -.
DR SMR; Q8RA32; -.
DR STRING; 273068.TTE1398; -.
DR PRIDE; Q8RA32; -.
DR EnsemblBacteria; AAM24620; AAM24620; TTE1398.
DR KEGG; tte:TTE1398; -.
DR eggNOG; COG2176; Bacteria.
DR HOGENOM; CLU_003297_2_0_9; -.
DR OMA; YYAAYFT; -.
DR OrthoDB; 561611at2; -.
DR Proteomes; UP000000555; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..1401
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_0000204606"
FT DOMAIN 388..543
FT /note="Exonuclease"
SQ SEQUENCE 1401 AA; 158806 MW; 49342F6617C8DB34 CRC64;
MVPATFLENM QIKKVRVEKK SRKLTVVVSS FSSNAQKLSE FQSFLEESFP SLKEIKIVVE
SPSLSTVEEV LENWEKVVLE LSEEYPSSLS FLKTCDVAKE GQNRITVKAP TYAIYEMAKS
SKLDFAIREF LRNRYELNLD VELIFSEEGE EIAEKIIEED IKAIEEVIQK DEKSKKEKSR
SEENRVLLGK EMKAKPISIK DVSAETDEVV IEGEIFSIDF KELKSKVLMV FDITDYTSSI
LVKTFLTEEK YEILKDEIDV GTFVRLRGNV IYDKYEGDLV IDLKDLELIP PKKRMDLSEE
KRVELHLHTQ MSTLDAVPSA TEVIKRAAEW GHKAVAITDH AVVQAFPEAM EASREYGVKV
IYGMEGYMVD DGIPIVTGES EASLEGEFVV FDIETTGLSN INDEIIEIGA VKIKNKKIVD
TFETFVNPQI PISSFITKLT GIDESMVKDA PLIEEVLPKF LEFAKGAVLV AHNANFDVSF
IKSKAKKLGL TVENTVLDTL ELSRHLYQDL KNYKLDTLAE FFEVKLLHHH RAVEDAKATA
EIFIKMLEKL QEIGIKSVSE INSVLMEREV DVKKLPVYHV TILVKDQKGL RNLYEIISRS
NLEFFHRTPR IPKSLLVKMR EGLIIGSACE QGEVFRALVS NLEEKKLEDI INFYDYLEIQ
PVGNNEFLIE RGEVRSVEEL KEINRKIYEL GKKYNKLVVA TGDVHFLDPW DDVYRKILMA
GKGYKDADRQ PPLYFRTTEE MLMEFEYLGE EAAREVVIEN PNKIAEIVED VKPIPEGTFP
PVIEGAEEEL RRITLEKAHE IYGDPLPPIV QERLDRELNA IINNGYAVMY VIAQKLVSKS
LQDGYLVGSR GSVGSSLVAT MSGITEVNPL PPHYVCPKCK HSEFVTDGSF GCGVDMPDKY
CPNCGTLMKK DGFDIPFEVF MGFEGDKEPD IDLNFSGEYQ PIAHRYTEEL FGKGHVFRAG
TIGTLADKTA YGYVKKYFEE RNLTVHKSEI KRLTMGCTGI KRTTGQHPGG VMVVPKDKSI
YDFTPIQRPA DAEDTDVITT HFDYHSLSGK LLKLDILGHD DPTVIRMLED LTGVNARKIP
LDDKKTMSLF TSVEALGIDP EELGTPVGTL GLPEFGTKFV RQMLIETRPT TFDELVRISG
LSHGTDVWLN NAQDIIREGI ATLKEVIAAR DDIMLYLISK GMDKKLSFKI MENVRKGKGV
TQEEIEEMKK HGVPDWFIQS CQKIKYMFPK AHAVAYVIMA FRIAYFKVYY PEAFYATYFT
VRADDFNLDI VLGGKESIKR AIKEIEAKGN NATPKEKNLL TVLEVALEMY LRGIKFTNVD
LYRSDAEKFL ITEEGLLPPL NSLEGVGIQA AKAIAQEREN GKFISIEDFR NRTRVSKTVI
EILKQYGCLE DLPESNQLSL F
