DPO3_LACLA
ID DPO3_LACLA Reviewed; 1638 AA.
AC Q9CDT7;
DT 04-MAY-2001, integrated into UniProtKB/Swiss-Prot.
DT 04-MAY-2001, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=LL2124;
GN ORFNames=L0278;
OS Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
OX NCBI_TaxID=272623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IL1403;
RX PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA Ehrlich S.D., Sorokin A.;
RT "The complete genome sequence of the lactic acid bacterium Lactococcus
RT lactis ssp. lactis IL1403.";
RL Genome Res. 11:731-753(2001).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK06222.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE005176; AAK06222.1; ALT_INIT; Genomic_DNA.
DR PIR; D86890; D86890.
DR RefSeq; NP_268281.1; NC_002662.1.
DR RefSeq; WP_031296849.1; NC_002662.1.
DR AlphaFoldDB; Q9CDT7; -.
DR SMR; Q9CDT7; -.
DR STRING; 272623.L0278; -.
DR PaxDb; Q9CDT7; -.
DR EnsemblBacteria; AAK06222; AAK06222; L0278.
DR KEGG; lla:L0278; -.
DR PATRIC; fig|272623.7.peg.2283; -.
DR eggNOG; COG2176; Bacteria.
DR HOGENOM; CLU_003297_2_0_9; -.
DR OMA; YYAAYFT; -.
DR Proteomes; UP000002196; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 1.
DR Gene3D; 2.40.50.140; -; 2.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..1638
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_0000204577"
FT DOMAIN 596..752
FT /note="Exonuclease"
FT REGION 193..212
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 194..212
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1638 AA; 184960 MW; D902C18EDE77FA8E CRC64;
MENLFEKLME QIKMPPNLRR SSQFEHADIE NVEVHTASKL WHFQLIFDEI LPIDTYKLLT
ELTETAFSTI ARTEISVSSR NQNIDEQNLN DYYQYALTLP ELCDSAFSSI FKKYHLEKEE
EKIHLMVEDN PQMDFFVEKY FPILEEKFKS FGFGEVRITP LVDQELTETQ AAAHAEKVAA
RLAAQTAEQA QISEIKKQRS EERESKNTRE AKPEFVETAL SDGIFFGRKI SGQSPITSMS
FIAGEGFGVI FEGLVFEAAH REFTGKESGK VNHILELKMA DETSTFMISK WGRKDEEIAQ
FDQIVSTVKA MNEKIIADRT DGEDNFTDSL SDALWLRVQG NIEHDKYKDD LVLTANAVVE
IKPKPTIDRV ALQIKAVKSD KIQLGREIKN NEPVTPMRSV SEFSTNGPVV FEGYVFKGEL
REIKSRKTGN ISYLLEFEMT DYTSSFYVQK WLRGEEEIQL AKQIKAGLWC RVRGNVQRDN
FKNDLVLQLT DLIEIPTQNV REDKSDEKRV EFHAHTNMSQ MDAIPSASSL VAQAAKWGHK
AIAITDHGGL QSFPEAHSAG KKNGVKIIYG VEANLVEDKI PIVYNETDVD MYESTYVVFD
VETTGLSAVH NDLIQIAATK MHKGNPIDEF DEFINPGYRL SEFTTELTGI TDDHVKNAKP
LYEVLTKFQK FCEGTILVAH NATFDVGFMN MNYSRNGLPI ITQPVVDTLE FARNLYPEMK
RFGLGQLTKK FQIGLEHHHM ANFDAEATGR LLFVFLEELR TRNTGWTSLL ELNDKLVSED
SYKKVRPKHV TLYAKTQEGL KNLFKIVSFG NVKYYAGLPR VPRSVLEANR EGLLVGSACE
IGEVFDAAIN KTFEETLEIA KFYDFIEVFP PALYRSLVVG GSFKSEKELE QTLKDLIKIG
KTLGKPVLAT GNLHYLNPED AIYREIIVRS LGLGAEINWT QGHGEHAKPL PLPEAHFRTT
DEMLEAFSFL DEKTAREIVI DNTQKMADEF DVLTPVRDDL YTPKMVFDGG ETSEERIVRL
TYEKAHEWYG NPLPDIIDAR LEKELRSILG NGFSVVYIIS QELVKRSNDR GYIVGSRGSV
GSSLVATMIG ITEVNPLAPH YRCPECQYFE CYDDGSFGSG YDMPDKNCPK CDHKLIKDGH
DIPFETFLGF KGDKVPDIDL NFSGDDQPLA HLDVRDIFGE DYAFRAGTIG TVAEKTAFGF
VKGYERDYDQ FYGNAEIDRL AAGSTGVKRT TGQHPGGIIV IPNYMDVYDF SPVQFPADDV
NAEWQTTHFD FHAIHDNILK LDILGHDDPT MIRKLQSLSG IVPQDIPMDD PGVMKLFTGT
ESLGLTEEQL GVKLGTLGIP EMGTFTSMNM IAEAKPKNFA DLLQISGLSH GTDVWSGNAQ
DLIRSGIADL SSVIGCRDDI MVYLIHKGLE NGLAFTIMER VRKGMWNKIP AEEREKYVEA
MREHDVPEWY IESCSKIKYM FPKAHAAAYI MMALRVAYFK VHHPILYYCA WFSIRATAFD
IGVMGAGLEA VKAKMKEIKD KGFDATNVET NLYTTLELCN EMLERGFTFG KIDLYRSEAT
EFVIDGDTLI PPFVTMDGLG ENVAKQIVAA RAEGEFLSKM ELRKRGGVSQ TIIENMTNMG
VLEGMPDDNQ LSLFDDFF
