DPO3_LACP3
ID DPO3_LACP3 Reviewed; 1444 AA.
AC Q038M0;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2006, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=LSEI_1578;
OS Lacticaseibacillus paracasei (strain ATCC 334 / BCRC 17002 / CCUG 31169 /
OS CIP 107868 / KCTC 3260 / NRRL B-441) (Lactobacillus paracasei).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lacticaseibacillus.
OX NCBI_TaxID=321967;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 334 / BCRC 17002 / CCUG 31169 / CIP 107868 / KCTC 3260 / NRRL
RC B-441;
RX PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT "Comparative genomics of the lactic acid bacteria.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR EMBL; CP000423; ABJ70352.1; -; Genomic_DNA.
DR RefSeq; WP_011674539.1; NC_008526.1.
DR RefSeq; YP_806794.1; NC_008526.1.
DR AlphaFoldDB; Q038M0; -.
DR SMR; Q038M0; -.
DR STRING; 321967.LSEI_1578; -.
DR PRIDE; Q038M0; -.
DR EnsemblBacteria; ABJ70352; ABJ70352; LSEI_1578.
DR KEGG; lca:LSEI_1578; -.
DR PATRIC; fig|321967.11.peg.1558; -.
DR HOGENOM; CLU_003297_0_0_9; -.
DR OMA; YYAAYFT; -.
DR Proteomes; UP000001651; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR SUPFAM; SSF89550; SSF89550; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Transferase.
FT CHAIN 1..1444
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_1000048473"
FT DOMAIN 421..577
FT /note="Exonuclease"
SQ SEQUENCE 1444 AA; 161523 MW; AF3B64689E8B6ABA CRC64;
MALSQHEMFE KLLDQLDLAA DVRQDPSLTS GTVQNVTIHE QSRRYDFTLG FDAILPFQTF
NAIATKLPLV FQQIAATDLS VVVTQPTITD ELLAQYWQYV VTHAEIGTGI VRELCEKQVP
TLENDRAVIK TENEQIRQYL IQQGLGKLEE TYRQVGFSGL RMQAEVDEEQ AAQSMAAFQA
QRAEETAKMA KAAAEVVKQQ AAKQKQVQTD GPVQMGRQMK MTDAPQQMVT ITQEERSVTV
EGYVFDVEVR ELRSKRQLLI FKVTDYSSSF IAKKFSNGPE DEAMFARIQK GQWLRVRGSV
QEDNYSRELT INAQDIQTVS HPDPTDDAEG EKRVELHLHT NMSQMDAMNP ISDYVKRAKE
WGHKAIAVTD HAGLQAYPEA HSAAVKAGLK MLYGVEINLV DDGTPVAYRA DEPRDLASAE
YVVFDVETTG LSAVYDKVIE LAAVKMKDGK VIDQFEEMID PGFPLSELTI NLTHITDDMV
HGSKSEVDVF KLFQQFCDGA IMVGHNVTFD VGFLDNGYER HGLADIDNPV IDTLELSRML
HPERKNHKLD TLAKQYKVSL EHHHRANADA EATGYLLYAL EKEAAKMYGM TTLNQLNDRV
GAGDAYKAAR PSHAIVFAKT QAGLKNLFKL VSLSNVKYFY RVPRVPRSQL QKLREGLLVG
SACSNGEVFT AMMQKGEAEA RAKASFYDYL EVQPLPVYQP LIEAGLIKGE AHLKDIIQKI
IKIGSELEKP VVATGDAHYL DQHDAIYRQI LIHSQGGANP LNRHSLPDVH FRSTSEMLTD
FSWLGEEKAH ELVVDNSNLI ANWVDDDITP VKDKLYTPEV PGVEENLKHD VMTTAHELYG
DPLPDIVAQR LDKELKSIIG NGFSVIYNIA QRLVLKSNKD GYLVGSRGSV GSSLAATMAG
ITEVNPLPPH YRCPNCQYSE FFTHGEIGSG FDLPDKQCPK CGADLHKDGH DIPFETFLGF
HGDKVPDIDL NFSGDYQPIA HNYIKVMFGE DHSFRAGTIG TVADKTAYGY VKAYERDTGQ
QLRGAEIDRL AQGDTGVKRT TGQHPAGILI VPADMDIYDF TPIQYPADDQ NAAWMTTHFD
FHSIHDNILK MDVLGHDDPT MIRMLQDLSG VEPKSIPTDD PGVMALFSGT KSLGVTPEQI
NSKMGTLGIP EFGTRFVRGM LEETKPTTFS ELLQISGLSH GTDVWLGNAE ELIKQGIVTL
KEVIGCRDNI MMDLIHWGME DSMAFNIMEH VRKGRGIPDD WQKAMRENEN VPDWYIESCL
KIKYMFPKAH ATAYILMALR IAWFKVHYPL IYYTAYFSVR AEDFDLAAMS HGKEAVKAAM
KEITDKGMDA STKEKQLLTV LEIANECLER GFKIKMIDVT KSDSHNFLIQ DDHTILAPFR
AVPGLGDNVA KQIVAAREEK PFLSKEDLAN RGKVSKTLID YMTTNHVLDD LPDENQLSLF
DGLF
