DPO3_LATSS
ID DPO3_LATSS Reviewed; 1446 AA.
AC Q38W73;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=LCA_1256;
OS Latilactobacillus sakei subsp. sakei (strain 23K) (Lactobacillus sakei
OS subsp. sakei).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Latilactobacillus.
OX NCBI_TaxID=314315;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=23K;
RX PubMed=16273110; DOI=10.1038/nbt1160;
RA Chaillou S., Champomier-Verges M.-C., Cornet M., Crutz-Le Coq A.-M.,
RA Dudez A.-M., Martin V., Beaufils S., Darbon-Rongere E., Bossy R., Loux V.,
RA Zagorec M.;
RT "The complete genome sequence of the meat-borne lactic acid bacterium
RT Lactobacillus sakei 23K.";
RL Nat. Biotechnol. 23:1527-1533(2005).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR EMBL; CR936503; CAI55560.1; -; Genomic_DNA.
DR RefSeq; WP_011374953.1; NC_007576.1.
DR AlphaFoldDB; Q38W73; -.
DR SMR; Q38W73; -.
DR STRING; 314315.LCA_1256; -.
DR EnsemblBacteria; CAI55560; CAI55560; LCA_1256.
DR KEGG; lsa:LCA_1256; -.
DR eggNOG; COG2176; Bacteria.
DR HOGENOM; CLU_003297_0_0_9; -.
DR OMA; YYAAYFT; -.
DR OrthoDB; 561611at2; -.
DR BioCyc; LSAK314315:LCA_RS06240-MON; -.
DR Proteomes; UP000002707; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR SUPFAM; SSF89550; SSF89550; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..1446
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_1000048475"
FT DOMAIN 425..581
FT /note="Exonuclease"
SQ SEQUENCE 1446 AA; 161867 MW; A931879D8C0C60F0 CRC64;
MALDQTALFQ KLLEQIALPA DVAHYPGFKT GQVEQVIVHE TSKRWTFKLH FDNVLPFAVY
TAFEEHLEAA FQAIAAVSIE ISTDAKELDG GVLAAYWEYV VNHSGIQSSL LHELCAKETP
YIEGRKVLLV VENDIVKTFF INQAQTTIQA GYKRLGFPNF AIQPLIDESA SQKSIEDFQA
KRAVQDAERA QAAAEAIKKN EALKEKRKTE GKPVDGPIVM GRGINPAEPV RQMISITEEE
RSIVIEGFIF DKEVRVLRSG RQLLILKMTD YSSSFTVKKF SRDASDESLF AAIDKGMWFK
VRGSVQEDNF MRDLTVNAND LVEVSHPKRE DTATDGKRIE MHLHTNMSQM DATNPIGDYV
KQAAKWGQPA IAVTDHYNLQ AFPDAYAAGK KNGVKILYGV EVNLVNDGTP VVYNLRDQVL
ESAEYVIFDV ETTGLSAVYD SIIELAAVKM RDGEVVASFD EFIDPERPLS AFTTQLTSIT
NEMVHGAKKE AEVLAMFKEF TGDAVLAGHN VSFDMGFLNA GYERNDIDLI DNPVIDTLEL
SRMLHPEYKN HKLDSLTKRY KINLEHHHRA NADAESTGYL LYKLEKEAAE NYDMVNVNQL
NDRVGVGEFY KQARPAHAVL MAQTQAGLKN LFKLVSASMT EYYYRTPRLP KSKLDALREG
ILVGSACSNG EVFEAMMQKG YNEALDRAKY YDYIEVMPKA VYAPLLEREL VRDNRALEEI
IRNLVKVGEK LNKPVVATGD AHYLNPEDAI YRKILIHSMG GANPLNRSKL PDVHFRSTDE
MLTAFDFLGP ELAQELVVAN PQKIADQIDE IVPVKDKLYT PKMAGAEDEI QTLTMNRAHE
LYGAELPEIV EARLKKELKS IIGNGFSVIY LISQKLVYKS GKDGYLVGSR GSVGSSLVAT
MTGITEVNPL PPHYRCSNCH YSEFFTKGEV GSGYDLADKD CPECGTPLDK DGHDIPFETF
LGFHGDKVPD IDLNFSGDYQ PVAHNYTKVL FGENNVFRAG TIGTVADKTA YGYVKAYERD
TEQTFRGAEV DRLAKGSTGV KRTTGQHPAG IIVVPDYMDI YDFTPIQFPA DDQDAAWKTT
HFDFHSIHDN ILKLDILGHD DPTMIRMLQD LSGIDPKSIP TDDPGVMALF SGTDSLGVTP
EQINSKMGTL GVPEFGTRFV RGMLEETHPT TFSELLQISG LSHGTDVWLG NAEELINKGV
VTLKDVIGCR DNIMMDLIHW GMDDSMSFNI MERVRKGKGI PDDWQQAMRD NENVPDWYID
SCLKIKYMFP KAHATAYILM ALRIAYFKVY FPIIYYCAYF SVRASDFDLV AMAQGKEGVK
ARMKEITDKG MEASTKEKNL LTVLEIANEC LERGITIKMV DIEKSDSSDF LIQDDHTLLA
PFRAVPSLGD NVAKQIVSAR EEKPFLSKED LSNRGKVSKT LIEYLTENNV LNDLPDENQL
SLFDMM
