DPO3_LEVBA
ID DPO3_LEVBA Reviewed; 1445 AA.
AC Q03QS9;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2006, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=LVIS_1341;
OS Levilactobacillus brevis (strain ATCC 367 / BCRC 12310 / CIP 105137 / JCM
OS 1170 / LMG 11437 / NCIMB 947 / NCTC 947) (Lactobacillus brevis).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Levilactobacillus.
OX NCBI_TaxID=387344;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 367 / BCRC 12310 / CIP 105137 / JCM 1170 / LMG 11437 / NCIMB
RC 947 / NCTC 947;
RX PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT "Comparative genomics of the lactic acid bacteria.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR EMBL; CP000416; ABJ64443.1; -; Genomic_DNA.
DR RefSeq; WP_011668016.1; NC_008497.1.
DR AlphaFoldDB; Q03QS9; -.
DR SMR; Q03QS9; -.
DR STRING; 387344.LVIS_1341; -.
DR PRIDE; Q03QS9; -.
DR EnsemblBacteria; ABJ64443; ABJ64443; LVIS_1341.
DR KEGG; lbr:LVIS_1341; -.
DR eggNOG; COG2176; Bacteria.
DR HOGENOM; CLU_003297_2_0_9; -.
DR OMA; YYAAYFT; -.
DR OrthoDB; 561611at2; -.
DR Proteomes; UP000001652; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..1445
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_1000048472"
FT DOMAIN 425..581
FT /note="Exonuclease"
FT REGION 193..223
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 198..213
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1445 AA; 161760 MW; CDF46156299456EC CRC64;
MDEDRHALFE KLLQQLDLTS EADQPYFQTA TVDRLTVHEQ SRRWQFLLKL PSMLPFTAFV
DFHNHLQVAF REIATVEATI EVDQPELTNR LLGDYWEWIV KNSGLTSNLI QELCQSQVPE
LDENNRVLLY AQNEVVKDFL SNQALGPIES AYRRAGFPKF NIHVMIDESK SQAKIDEFKA
RQEKTDAQLA QQAAAAIEKA NQKRQSQGDT PAAEGPTQIG KAIKGDQPVT KMIDITQEER
SVVVEGYVFE KEVRKLRSGR QLLTLKITDY TSSFAVKKFS RGAEDEAQFA GVEEGSWVKV
RGNVQEDTYM HDLALNAYDI NQVKHASRQD TAPEGEKRVE LHLHTSMSQM DATNSIGDYV
KQAKKWGMPA LAITDHADVQ GFPAAFNSAA KAGIKMLYGV EANLVDDGVP IGYNSAHVPL
DGATYVVFDV ETTGLSAIYD KVIELSAVKM EHQNVVDQFE EFIDPGFPLS EQTTNLTSIT
DDMVAGSKSE EEVFKLFREF CGDAIIVGHN VTFDVGFMNT GYQRHGMAEI SNPIIDTLTL
ARFLYPTLKS YRLNTLAKRF HVSLEHHHRA VYDAESTGHL NYLFLKDAED RYDIQYHDQL
NDHMTDNDAY KHARPNHAIL LAKTQAGLKN MFKLVSASNV DYYYRVPRVP RSVLEEYREG
IIVGSACSSG EVFTAMMQKG QVEAAAKAKF YDYLEVQPSA AYQPLIDSGL IADRENMEEI
VRNMVKLGHD LNKPVVATGD VHFLNPDDYI YRKILIHSQG GANPLNRQEL PDVHFMTTDE
MLTDFAYLGE ETAKEIVVTN TQKIADDIDE VHPLKDKLYT PRMEGAEDEI RQRTMDTAHA
MYGDPLPELV QHRVDRELKS IIGNGFSVIY LIAQRLVAKS NKDGYLVGSR GSVGSSLVAT
LTGITEVNPL PPHYRCPKCQ YSHFYTKGEY SSGYDLPEKD CPECGTLMIG DGHNIPFETF
LGFKGNKVPD IDLNFSGDYQ PIAHNYTKVL FGEKNVYRAG TIGTVADKTA YGYVKAYERD
TEQTFRGAEI DRLAKGATGV KRTTGQHPAG IIVVPDYMDI FDFTPIQYPA DDQSAAWQTT
HFDFHSIHDN ILKIDILGHD DPTMIRTLQD LSGVDPTTIP MDDPGVMGLF SGTDSLGVTP
EQIQSKTGTL GVPEFGTRFV RGMLEETHPQ NYSQLLQISG LSHGTDVWLG NAEELIKNGT
ATIANVIGCR DNIMTDLINF GLDSETSFQV MEHVRKGRGI PDEWQEKMKA TDCPDWYIDS
CLKIKYMFPR AHAAAYVLMA LRVAYFKVYF PIIYYAAYFS VRADDFDVVA MSQGKTAVKA
AMKAINDQGM DASAKDKNLL TVLELANEML ERGFKFKMID LEKSDAADWI IDGDTLIAPF
KAAPGLGLNV AKQIVAARND RPFISKEDLA KRGKVSKTLI DFMTENGVLA GLPDENQLSL
FDDLM
