DPO3_LISIN
ID DPO3_LISIN Reviewed; 1444 AA.
AC Q92C34;
DT 27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=lin1357;
OS Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX NCBI_TaxID=272626;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-680 / CLIP 11262;
RX PubMed=11679669; DOI=10.1126/science.1063447;
RA Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F.,
RA Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A.,
RA Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E.,
RA Dominguez-Bernal G., Duchaud E., Durant L., Dussurget O., Entian K.-D.,
RA Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W.,
RA Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U.,
RA Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A.,
RA Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B.,
RA Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N.,
RA Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P.;
RT "Comparative genomics of Listeria species.";
RL Science 294:849-852(2001).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR EMBL; AL596168; CAC96588.1; -; Genomic_DNA.
DR PIR; AD1602; AD1602.
DR RefSeq; WP_003762047.1; NC_003212.1.
DR AlphaFoldDB; Q92C34; -.
DR SMR; Q92C34; -.
DR STRING; 272626.lin1357; -.
DR EnsemblBacteria; CAC96588; CAC96588; CAC96588.
DR KEGG; lin:polC; -.
DR eggNOG; COG2176; Bacteria.
DR HOGENOM; CLU_003297_0_0_9; -.
DR OMA; YYAAYFT; -.
DR OrthoDB; 561611at2; -.
DR Proteomes; UP000002513; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 2.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Transferase.
FT CHAIN 1..1444
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_0000204579"
FT DOMAIN 428..584
FT /note="Exonuclease"
SQ SEQUENCE 1444 AA; 162905 MW; D4B78BCA39D9AD95 CRC64;
MTAKEEEKQE RFQLLMTQIG LQDVTTYEEF TKDAKIEKLV ADKKNKTWQF HLHVPQIFPA
ALFHMMDVGM KRAFSQIAET EMQIVPENQT INETLIQEYW NLIVEPIGKQ SPMIGKLLME
QKPTFKEPHF IEVAVHNDME EATIQQRFQA KIIENYGKAG FPRLAMKMHM LDQSETDEYK
AFAQAKQEED QKKAAEAVQV MQKRQAEGQN GSANAAPLTG PFQIGYKIKD DEEIKRLGDV
YDEERRITVQ GLIFATEIRE LRSGRSLLQF KITDYTSSMI IKMFSRDNED AAMFQNLKKG
MWVKVRGSVQ NDTFVRDLIM MAQDINEIAG VKRLDTAEEK RAELHLHSPM SQMDATSSVD
SLFKQAADWG HKAIAITDHS VAQSFPEAYG AGQKYGLKVI FGIEANLIDD GVPIAYNDQH
IALEDATYCV FDVETTGLSA VYDTIIELAG VKMKNGEIID KFEAFIDPGH PLSATTINLT
GITDDMVKGS DPIDVVLKQF REWSGDDILV AHNASFDMGF INTAYEKVGL EKAKNAVVDT
LELARFLYPH FKNHRLNTLT KKFNIILEQH HRAVFDAEAT AYLAWKLIKD AKEMHDIEFH
DSLNDYMGEG DAYKRARPFH ATIYAQTDVG LKNLFKLITM SNINYFYRVP RIPRSQLKKM
REGLIIGTAC SQGELFEAMM QKGMQAAEKV AEFYDFIEVQ PKPVYAPLIE RELVRDEKAL
EEILKNIVRV GEKTGKPVVA TGNVHYKDPV DKIYRKILIH SQGGANPLNR AELPDVHFRS
TDEMLKEFAF LGEEKAKEIV VTNSNLVVDW MDDLKPIKDE LYTPKIDGAE DEVRNMSYGM
AHQLYGEKLP EIVEARLEKE LKSIIGHGFA VIYLISHKLV KKSLVDGYLV GSRGSVGSSF
VATMTEITEV NPLPPHYLCP NCKDSEFFDD GSVGSGFDLP DKDCPHCGTP YQKEGQDIPF
ETFLGFKGDK VPDIDLNFSG DYQPVAHAYT KEIFGEDYVF RAGTIGTVAE KTAFGYVRNY
ERDMNMTIRG AEVDRLVAGC TGVKRTTGQH PGGIIVIPDY MDVYDFTPVQ FPADATDSEW
KTTHFDFHSI HDNVLKLDIL GHDDPTAIRM LQDLSGIDPK TIPTDDPDVM KLFGSTESLG
VKPADIDSKT GTLGIPEFGT RFVRQMLEQT KPTTFSELVQ ISGLSHGTDV WLGNAEELIK
NKTCELPDVI GCRDDIMVFL IYQGLESSLA FKIMESVRKG KGLTEEMEEA MIANKVPLWY
IESCKKIKYM FPKAHAAAYV LMAVRIAYFK VHHPLYFYAT YFTVRADDFD LTSMVNGKEA
VKATMKEVND KGMEASTKEK NLLTVLEIAN EMLARGFHFQ KVDLYKSSAD EFIIDGDSLI
PPFNAIPSLG TNVAKQIVAA RENGEFLSKE DLQQRGKVSK TIIQYMDDQG CLEGLPDQNQ
LSLF
