DPO3_LISMF
ID DPO3_LISMF Reviewed; 1444 AA.
AC Q720A2;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356};
GN OrderedLocusNames=LMOf2365_1337;
OS Listeria monocytogenes serotype 4b (strain F2365).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX NCBI_TaxID=265669;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=F2365;
RX PubMed=15115801; DOI=10.1093/nar/gkh562;
RA Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F.,
RA Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D.,
RA White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M.,
RA Daugherty S.C., Dodson R.J., Durkin A.S., Madupu R., Haft D.H.,
RA Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A.,
RA Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O.,
RA Luchansky J.B., Fraser C.M.;
RT "Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne
RT pathogen Listeria monocytogenes reveal new insights into the core genome
RT components of this species.";
RL Nucleic Acids Res. 32:2386-2395(2004).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR EMBL; AE017262; AAT04112.1; -; Genomic_DNA.
DR RefSeq; WP_003726417.1; NC_002973.6.
DR AlphaFoldDB; Q720A2; -.
DR SMR; Q720A2; -.
DR KEGG; lmf:LMOf2365_1337; -.
DR HOGENOM; CLU_003297_2_0_9; -.
DR OMA; YYAAYFT; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 2.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Transferase.
FT CHAIN 1..1444
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_0000204580"
FT DOMAIN 428..584
FT /note="Exonuclease"
SQ SEQUENCE 1444 AA; 162742 MW; 8A11F5E2B6E0F76A CRC64;
MTAKEEEKQE RFQLLMTQIG LQDVTTYEEF TKDAKIEKLV ADKKNKTWQF HLHVPQIFPA
ALFHMMDVGM KRAFSQIAET EMQIVPENQT IDETLIQDYW NLIVEPIGKQ SPMIGKLLME
QKPTFKEPHF IEVAVHNDME EATIQQRFQT KIIESYGKAG FPRLAMKMHL LDQSETEEYK
AFALAKQEED QKKAAEAVQV MQKRQAEGQS GGGGAAPLTG PFQIGYKIKD DEEVKRLGDI
YDEERRITVQ GLIFATEIRE LRSGRSLLQF KITDYTSSMI IKMFSRDNED AAMFQNLKKG
MWVKVRGSVQ NDTFVRDLIM MAQDVNEIAG VKRLDTAEEK RAELHLHSPM SQMDATSSVD
SLFKQAADWG HKAIAITDHS VAQSFPEAYG AGQKYGLKVI FGIEANLIDD GVPIAYNDQH
IALEDATYCV FDVETTGLSA VYDTIIELAG VKMKNGEIID KFEAFIDPGH PLSATTINLT
GITDDMVKGS DPIDVVLKRF KEWSGDDILV AHNASFDMGF INTAYEKVGL EKAENAVVDT
LELARFLYPH FKNHRLNTLT KKFNIILEQH HRAVFDAEAT AYLAWKLIKD AKEMHNIDFH
DSLNDYMGEG DAYKRARPFH ATIYAQTAVG LKNLFKLITM SNINYFYRVP RIPRSQLKKL
REGLIIGTAC SQGELFEAMM QKGMQAAEKV AEFYDFIEVQ PKPVYAPLIE RELVRDEKAL
EEILKNIVRV GEKTGKPVVA TGNVHYKDPV DKIYRKILIH SQGGANPLNR AELPDVHFRT
TDEMLKEFAF LGEEKAKEIV VTNANLVVDW MEDLKPIKDE LYTPKIDGAE DEVRNMSYAM
AHQLYGEKLP EIVEARLEKE LKSIIGHGFA VIYLISHKLV KKSLVDGYLV GSRGSVGSSF
VATMTEITEV NPLPPHYLCP NCKDSEFFDD GSVGSGFDLP DKDCPHCGTP YQKEGQDIPF
ETFLGFKGDK VPDIDLNFSG DYQPVAHAYT KEIFGEDYVF RAGTIGTVAE KTAFGYVRNY
ERDMNMTIRG AEIDRLVAGC TGVKRTTGQH PGGIIVIPDY MDVYDFTPVQ FPADATDSEW
KTTHFDFHSI HDNVLKLDIL GHDDPTAIRM LQDLSGIDPK TIPTDDPDVM KLFGSTESLG
VKPADIDSKT GTLGIPEFGT RFVRQMLEQT KPTTFSELVQ ISGLSHGTDV WLGNAEELIK
NKTCELPDVI GCRDDIMVFL IYQGLESSLA FKIMESVRKG KGLTEEMEEA MMANKVPLWY
IESCKKIKYM FPKAHAAAYV LMAVRIAYFK VHYPLYFYAT YFTVRADDFD LTSMVNGKEA
VKATMKEVND KGMEASTKEK NLLTVLEIAN EMLARGFHFQ KVDLYKSSAD EFIIDGDSLI
PPFNAIPSLG TNVAKQIVAA RENGEFLSKE DLQQRGKVSK TIIQYMDDQG CLEGLPDQNQ
LSLF
