DPO3_LISMO
ID DPO3_LISMO Reviewed; 1444 AA.
AC Q8Y7G1;
DT 27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=lmo1320;
OS Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX NCBI_TaxID=169963;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-679 / EGD-e;
RX PubMed=11679669; DOI=10.1126/science.1063447;
RA Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F.,
RA Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A.,
RA Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E.,
RA Dominguez-Bernal G., Duchaud E., Durant L., Dussurget O., Entian K.-D.,
RA Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W.,
RA Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U.,
RA Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A.,
RA Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B.,
RA Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N.,
RA Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P.;
RT "Comparative genomics of Listeria species.";
RL Science 294:849-852(2001).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR EMBL; AL591978; CAC99398.1; -; Genomic_DNA.
DR PIR; AH1239; AH1239.
DR RefSeq; NP_464845.1; NC_003210.1.
DR RefSeq; WP_010989735.1; NZ_CP023861.1.
DR AlphaFoldDB; Q8Y7G1; -.
DR SMR; Q8Y7G1; -.
DR STRING; 169963.lmo1320; -.
DR PaxDb; Q8Y7G1; -.
DR EnsemblBacteria; CAC99398; CAC99398; CAC99398.
DR GeneID; 987700; -.
DR KEGG; lmo:lmo1320; -.
DR PATRIC; fig|169963.11.peg.1357; -.
DR eggNOG; COG2176; Bacteria.
DR HOGENOM; CLU_003297_2_0_9; -.
DR OMA; YYAAYFT; -.
DR PhylomeDB; Q8Y7G1; -.
DR BioCyc; LMON169963:LMO1320-MON; -.
DR Proteomes; UP000000817; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 2.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..1444
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_0000204581"
FT DOMAIN 428..584
FT /note="Exonuclease"
SQ SEQUENCE 1444 AA; 162733 MW; D8B3F57FBDF3815B CRC64;
MTAKEEEKQE RFQLLMTQIG LQDVTTYEEF TKDAKIEKLV ADKKNKTWQF HLHVPQIFPA
ALFHMMDVGM KRAFSQIAET EMQIVPENQT INETLIQDYW NLIVEPIGKQ SPMIGKLLME
QKPTFKEPHF IEVAVHNDME EATIQQRFQT KIIESYGKAG FPRLAMKMHM LDQSETDEYK
AFAQAKQEED QKKAAEAVQV MQKRQAEGQS GGGGAAPLTG PFQIGYKIKD DEEVKRLGDI
YDEERRITVQ GLIFATEIRE LRSGRSLLQF KITDYTSSMI IKMFSRDNED AAMFQNLKKG
MWVKVRGSVQ NDTFVRDLIM MAQDVNEIAG VKRLDTAEEK RAELHLHSPM SQMDATSSVD
SLFKQAADWG HKAIAITDHS VAQSFPEAYG AGQKYGLKVI FGIEANLIDD GVPIAYNDQH
IALEDATYCV FDVETTGLSA VYDTIIELAG VKMKNGEIID KFEAFIDPGH PLSATTINLT
GITDDMVKGS DPIDVVLKRF KEWSGDDILV AHNASFDMGF INTAYEKVGL EKAENAVVDT
LELARFLYPH FKNHRLNTLT KKFNIILEQH HRAVFDAEAT AYLAWKLIKD AKEMHNIDFH
DSLNDYMGEG DAYKRARPFH ATIYAQTAVG LKNLFKLITM SNINYFYRVP RIPRSQLKKL
REGLIIGTAC SQGELFEAMM QKGMQAAEKV AEFYDFIEVQ PKPVYAPLIE RELVRDEKAL
EEILKNIVRV GEKTGKPVVA TGNVHYKDPV DKIYRKILIH SQGGANPLNR AELPDVHFRT
TDEMLKEFAF LGEEKAKEIV VTNANLVVDW MENLKPIKDE LYTPKIDGAE DEVRNMSYAM
AHQLYGEKLP EIVEARLEKE LKSIIGHGFA VIYLISHKLV KKSLVDGYLV GSRGSVGSSF
VATMTEITEV NPLPPHYLCP NCKDSEFFDD GSVGSGFDLP DKDCPHCGTA YQKEGQDIPF
ETFLGFKGDK VPDIDLNFSG DYQPVAHAYT KEIFGEDYVF RAGTIGTVAE KTAFGYVRNY
ERDMNMTIRG AEIDRLVAGC TGVKRTTGQH PGGIIVIPDY MDVYDFTPVQ FPADATDSEW
KTTHFDFHSI HDNVLKLDIL GHDDPTAIRM LQDLSGIDPK TIPTDDPDVM KLFGSTESLG
VKPADIDSKT GTLGIPEFGT RFVRQMLEQT KPTTFSELVQ ISGLSHGTDV WLGNAEELIK
NKTCELPDVI GCRDDIMVFL IYQGLESSLA FKIMESVRKG KGLTDEMEEA MMANKVPLWY
IESCKKIKYM FPKAHAAAYV LMAVRIAYFK VHYPLYFYAT YFTVRADDFD LTSMVNGKEA
VKATMKEVND KGMEASTKEK NLLTVLEIAN EMLARGFHFQ KVDLYKSSAD EFIIDGESLI
PPFNAIPSLG TNVAKQIVAA RENGEFLSKE DLQQRGKVSK TIIQYMDDQG CLEGLPDQNQ
LSLF
