ID   DPO3_LISW6              Reviewed;        1444 AA.
AC   A0AIC1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2006, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE            Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN   Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=lwe1335;
OS   Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / CIP 8149 /
OS   NCTC 11857 / SLCC 5334 / V8).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX   NCBI_TaxID=386043;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35897 / DSM 20650 / CIP 8149 / NCTC 11857 / SLCC 5334 / V8;
RX   PubMed=16936040; DOI=10.1128/jb.00758-06;
RA   Hain T., Steinweg C., Kuenne C.T., Billion A., Ghai R., Chatterjee S.S.,
RA   Domann E., Kaerst U., Goesmann A., Bekel T., Bartels D., Kaiser O.,
RA   Meyer F., Puehler A., Weisshaar B., Wehland J., Liang C., Dandekar T.,
RA   Lampidis R., Kreft J., Goebel W., Chakraborty T.;
RT   "Whole-genome sequence of Listeria welshimeri reveals common steps in
RT   genome reduction with Listeria innocua as compared to Listeria
RT   monocytogenes.";
RL   J. Bacteriol. 188:7405-7415(2006).
CC   -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC       also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC       Rule:MF_00356}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00356};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR   EMBL; AM263198; CAK20753.1; -; Genomic_DNA.
DR   RefSeq; WP_011702138.1; NC_008555.1.
DR   AlphaFoldDB; A0AIC1; -.
DR   SMR; A0AIC1; -.
DR   STRING; 386043.lwe1335; -.
DR   EnsemblBacteria; CAK20753; CAK20753; lwe1335.
DR   GeneID; 61189212; -.
DR   KEGG; lwe:lwe1335; -.
DR   eggNOG; COG2176; Bacteria.
DR   HOGENOM; CLU_003297_0_0_9; -.
DR   OMA; YYAAYFT; -.
DR   OrthoDB; 561611at2; -.
DR   Proteomes; UP000000779; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.150.700; -; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   HAMAP; MF_00356; DNApol_PolC; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR024754; DNA_PolC-like_N_II.
DR   InterPro; IPR028112; DNA_PolC-type_N_I.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR006054; DnaQ.
DR   InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR006308; PolC_gram_pos.
DR   InterPro; IPR044923; PolC_middle_finger_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF14480; DNA_pol3_a_NI; 1.
DR   Pfam; PF11490; DNA_pol3_a_NII; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 2.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF00929; RNase_T; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00479; EXOIII; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   TIGRFAMs; TIGR00573; dnaq; 1.
DR   TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW   Hydrolase; Nuclease; Nucleotidyltransferase; Transferase.
FT   CHAIN           1..1444
FT                   /note="DNA polymerase III PolC-type"
FT                   /id="PRO_1000048476"
FT   DOMAIN          428..584
FT                   /note="Exonuclease"
FT   REGION          196..218
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        203..218
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1444 AA;  162974 MW;  A1DA1BEBC0E3CF04 CRC64;
     MTAKEEEKQE RFQLLMTQIG LQDVTTYEEF TKDAKIEKLI ADKKNKTWQF HLHVPQIFPA
     PLFHMMDVGM KRAFSQIAET EMQIVPENQT INETLIQEYW NLIVEPIGKQ SPMIGKLLME
     QKPTFKEPHF IEVAVHNDME EATIQQRFQA KIIENYGKAG FPRLAMKMHM LDQSESDEYK
     AFAQAKQEED QKKAAEAVQV MQKRQAEGQN GNSSAAPLSG PFQIGYKIKD DEEIKRLGDV
     YDEERRITVQ GLIFATEIRE LRSGRSLLQF KITDYTSSMI IKMFSRDNED AAMFQNLKKG
     MWVKVRGSVQ NDTFVRDLIM MAQDINEIAG VKRLDTAEEK RAELHLHSPM SQMDATSSVD
     SLFKQAADWG HKAIAITDHS VAQSFPEAYG AGQKYGLKVI FGIEANLIDD GVPIAYNDQH
     IGLQDATYCV FDVETTGLSA VYDTIIELAG VKMKNGEIID KFEAFIDPGH PLSATTINLT
     GITDDMVKGS DPIDVVLKRF KEWSGDDILV AHNASFDMGF INTAYEKVGL EKADNAVVDT
     LELARFLYPH FKNHRLNTLT KKFNIILEQH HRAVFDAEAT AYLAWKLIKD AKEMHDINFH
     DSLNDYMGEG DAYKRARPFH ATIYAQTDVG LKNLFKLITM SNINYFYRVP RIPRSQLKKL
     REGLIVGTAC SQGELFEAMM QKGMQAAEKV VEFYDFIEIQ PKPVYAPLIE RELVRDEKAL
     EEILKNIVRV GEKAGKPVVA TGNVHYKDPV DKIYRKILIH SQGGANPLNR AELPDVHFRS
     TDEMLKEFAF LGEEKAKEVV VTNSNLVVDW MEELKPIKDE LYTPKIDGAE DEVRNMSYDM
     AHQLYGENLP EIVEARLEKE LKSIIGHGFA VIYLISHKLV KKSLVDGYLV GSRGSVGSSF
     VATMTEITEV NPLPPHYLCP NCKDSEFFDD GSVGSGFDLP DKECPHCGTA YQKEGQDIPF
     ETFLGFKGDK VPDIDLNFSG DYQPVAHAYT KEIFGEDYVF RAGTIGTVAE KTAFGYVRNY
     ERDMNMTIRG AEIDRLVAGC TGVKRTTGQH PGGIIVIPDY MDVYDFTPVQ FPADATDSEW
     KTTHFDFHSI HDNVLKLDIL GHDDPTAIRM LQDLSGIDPK TIPTDDPDVM KLFGSTESLG
     VKPADIDSKT GTLGIPEFGT RFVRQMLEQT KPTTFSELVQ ISGLSHGTDV WLGNAEELIK
     NKTCELPDVI GCRDDIMVFL IYQGLESSLA FKIMESVRKG KGLTEEMEEA MMANKVPLWY
     IESCKKIKYM FPKAHAAAYV LMAVRIAYFK VHYPLYFYAT YFTVRADDFD LTSMVNGKEA
     VKATMKEVND KGMEASTKEK NLLTVLEIAN EMLARGFHFQ KVDLYKSSAD EFIIDGDSLI
     PPFNAIPSLG TNVAKQIVAA RENGEFLSKE DLQQRGKVSK TIIQYMDDQG CLEGLPDQNQ
     LSLF