ID   DPO3_MYCGE              Reviewed;        1451 AA.
AC   P47277;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   03-AUG-2022, entry version 133.
DE   RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE            Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN   Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=MG031;
OS   Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
OS   (Mycoplasmoides genitalium).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=243273;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX   PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA   Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA   Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA   Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA   Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA   Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA   Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT   "The minimal gene complement of Mycoplasma genitalium.";
RL   Science 270:397-403(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 492-567; 975-1103 AND 1111-1225.
RC   STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX   PubMed=8253680; DOI=10.1128/jb.175.24.7918-7930.1993;
RA   Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT   "A survey of the Mycoplasma genitalium genome by using random sequencing.";
RL   J. Bacteriol. 175:7918-7930(1993).
CC   -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC       also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC       Rule:MF_00356}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00356};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L43967; AAC71247.1; -; Genomic_DNA.
DR   EMBL; U01807; AAD12336.1; -; Genomic_DNA.
DR   EMBL; U01712; AAC43185.1; -; Unassigned_DNA.
DR   EMBL; U02208; AAD12500.1; -; Genomic_DNA.
DR   PIR; D64203; D64203.
DR   RefSeq; WP_010869296.1; NC_000908.2.
DR   AlphaFoldDB; P47277; -.
DR   SMR; P47277; -.
DR   STRING; 243273.MG_031; -.
DR   EnsemblBacteria; AAC71247; AAC71247; MG_031.
DR   KEGG; mge:MG_031; -.
DR   eggNOG; COG2176; Bacteria.
DR   HOGENOM; CLU_003297_1_0_14; -.
DR   OMA; YYAAYFT; -.
DR   OrthoDB; 561611at2; -.
DR   BioCyc; MGEN243273:G1GJ2-31-MON; -.
DR   Proteomes; UP000000807; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.150.700; -; 2.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   HAMAP; MF_00356; DNApol_PolC; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR006054; DnaQ.
DR   InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR004805; PolC_alpha.
DR   InterPro; IPR006308; PolC_gram_pos.
DR   InterPro; IPR044923; PolC_middle_finger_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 2.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF00929; RNase_T; 1.
DR   SMART; SM00479; EXOIII; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   TIGRFAMs; TIGR00573; dnaq; 1.
DR   TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW   Hydrolase; Nuclease; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1451
FT                   /note="DNA polymerase III PolC-type"
FT                   /id="PRO_0000204582"
FT   DOMAIN          416..575
FT                   /note="Exonuclease"
SQ   SEQUENCE   1451 AA;  167500 MW;  2DB9E6951F41AAEF CRC64;
     MVFNLENEKD KKVLALSNFL IDNNILDHNE LNSLIERIES IYFNKYIEEK VFLFAITISR
     PLTIDIWNAL YEGFNELNEG FKADNESFKL TITFKENEPF FKSKNSFSSV TIAIIKDYFH
     SFFSKDKKYK ILIEQELSNP NFLSYENDEL KAQCQTKELT EWLVQKSKSF IFWMNNAGFK
     NFNFIALYPI DKNESKLKVK AVTVSQYDKQ FETKVFATEF IPIHKINQQI DDVKIIGQIF
     ELKTHESLTG KKTLNIYVTD FQLGGSLILK WSYTDEKKIE GITIGNWIKA HIQVERDPNT
     QILYGIVREI NPVEIPNNYK RLDLSKQKRV ELVFHTKMTA FDGINDIEEY AQFAKERGWK
     AITVTDKDNI HIYPKFYEVA KKYDLKAIYG LEFNLTDDHI KIVHNPDNTK LSDATFVIFD
     IETTGLHGRY DDVIEFSARK IKNNSEIDHQ QFFLKIDKPI PKTITEITKI TDEMLEGGID
     QQQGLEKIRN YLDDCVMVAH NGINFDLPFL QTQFEKYNIK PLTNPLIDTL CLSWALNPLF
     SSHTLSNICS KLKLEFDDER LHRAEYDTEA LKKVFFYFKK QLKEMGINTL TEIDQNLNKK
     CQIDLMKRVF TNTAIVYVKN QRGFQNLYEM LSIALTDHNA NRPLVLASSL AKFRKSFLLT
     ENPVQGDIFK AALTKPINEL EKAIEKVDFV LISQPNAYLG YTLREGLKKE LINDAIKLVI
     KTATKLKKLV AVASDAYFIH PWENEYYKAI VCAKGLGGKW HRHFNNKEKE QTVPEVFLHT
     TDEMLKRMSF LGEDIAYKLV VENTNKIVKL LDLNELVPTK NKLYPPVMQD SNQKLIDKTW
     KQAEKRYGKN LPKLIKERIE KELNAIISNG FGIVFWISHL LVEQSVKDGY FVGPRGSIGS
     SLIANLIGIS EINPLAAHYL CEQCHYFEVS DSVDDGYDLM IRDCPKCHEK ASFKGDGHNI
     PFATFMGFSG DKIPDIDLNF SSEYQAKAHD YVRKLFGVNN TFRAGTIATV AEKTAYGYAR
     NYFEIIKRVD LATTAEIERF KQKLIGIKRT TGQHPGGIMI FPSDHSVYEF TPCGFPADDV
     ESEWKTTHFE YDALGDAILK LDILGQDDPT MLKHLADLTK INPQNIPHFD KNLISMFSSN
     KPLNLKPGIV DEVTGAVGIP EFGTKFVRKI LEQTKPKDFA DLIRVSGLSH GKNVWADNAQ
     KLIKSNRLTL RDVIACRDDI MLYLINKGMQ AKDAFEIMEK VRKGIKVNAK EVSLMQNCGV
     EQYWINACLK INYLFPKAHA AAYVLMAWRI AWFKLYHPLS YYACLLSFKL KEHDINGFEK
     GYEFIKNRLD ELNKLYRIKK IKPKEAELLT SYEVYLEMMA RNIKLQQISI QNSNARMFVE
     HNGVLIAPFI TIPGMGEAVA SSIVEARNEK PFASLNDFKK RTKITKKHVE TMEQLQLFDE
     FEHQDDHKLF N