DPO3_MYCPN
ID DPO3_MYCPN Reviewed; 1443 AA.
AC P75080;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=MPN_034;
GN ORFNames=MP120;
OS Mycoplasma pneumoniae (strain ATCC 29342 / M129) (Mycoplasmoides
OS pneumoniae).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=272634;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29342 / M129;
RX PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.;
RT "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT pneumoniae.";
RL Nucleic Acids Res. 24:4420-4449(1996).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- INTERACTION:
CC P75080; P78014: recA; NbExp=3; IntAct=EBI-2258713, EBI-2258718;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR EMBL; U00089; AAB95768.1; -; Genomic_DNA.
DR PIR; S73446; S73446.
DR RefSeq; NP_109722.1; NC_000912.1.
DR RefSeq; WP_010874391.1; NC_000912.1.
DR AlphaFoldDB; P75080; -.
DR SMR; P75080; -.
DR IntAct; P75080; 4.
DR STRING; 272634.MPN_034; -.
DR PRIDE; P75080; -.
DR EnsemblBacteria; AAB95768; AAB95768; MPN_034.
DR KEGG; mpn:MPN_034; -.
DR PATRIC; fig|272634.6.peg.33; -.
DR HOGENOM; CLU_003297_1_0_14; -.
DR OMA; YYAAYFT; -.
DR BioCyc; MPNE272634:G1GJ3-48-MON; -.
DR Proteomes; UP000000808; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 2.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..1443
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_0000204583"
FT DOMAIN 408..567
FT /note="Exonuclease"
SQ SEQUENCE 1443 AA; 165102 MW; 5E8593E15B289C48 CRC64;
MVFDTETEKG KRIWALSQFL VKKNILDHNE LHQLNNRIEL VYLENDRENA LFLVALTLKK
PLTIDIWNAL YEGFQDAEGA ELRITFQEDA TFFKDGSTKS SVTLAIIKDY FKSFFGKDKK
YRILLEQELT HPNFLSYSNH ELKANCQSQE LDQWLIEQRQ AFIKWMHQAG FTHFGFVSLF
NPPAEKQLKV KSMKVSKYDK QFETEVFSTE FVPIHKINQQ MDEIKLMGQI FELKDFPGYN
NLRNTLNIYV TDFQLGGSLI LKWFYKDPKT IEGIKIGTWV KATVKVERDA KTQLLQGIIK
EISPIETPAY YRRPDQDKQK RVELVFHTKM SAFDGINSVQ EYAQFAKERD WKTIAVTDKD
NIHIYPTLYE VAKKYGLKAI YGLECNLIDD HIKIVSNPDK TKLKDATFVI FDIETTGLHG
RYDSVIEFAG IKVKHNREVE RMQFFLKIDG PLPAAVTEIT KITQAQLEDG MEQQAGLEKL
RAWLDGCVMV AHNGLSFDLP FLQTQFEKYN IAPLTNPLID TLALSWALNP GFASHTLSNI
CAKLKFDFDD ERLHRADYDT QALKKVFDYF KEQVELMGIT NLEQLDQELN QQCHFELLKR
TFTNTGIIYI KSQSGFAKLY ELLSIALTDN NATRPLVLTS TLQKFAKSFV ITDNPVQGDI
FKAALTKPLK ELEAAIKRVD FVLIAPPGAY AGYTIREGLK KEAIPNAIKL VVDTAQRLNK
LVAVASDAYF IHPWENEYYK AIVCAKGLGG RWHRHFNYKE REQRVPNVFV RTTGEMLNEM
SFLGEQLAYE LVVENTNKLA KQLTADDLVP VQTKLQPPVI EGSNENLAAK TWSQAKAIYG
DPLPKLIEQR IQEELKAIID NGFGIIYWIS HLLVKQSVQD GYFVGPRGSI GSSLVANLIG
ISEINPLVPH YLCESCQYFE VNEEVDDGYD LMVRDCPKCG AKAAFKGDGH NIPFATFMGF
AGDKIPDIDL NFSSEYQAKA HAYVRELFGE QYTFRAGTIA TVAEKTAYGY ARNYFEIIKQ
TELATAPEIE RFKQKLVGIK RTTGQHPGGI MIFPNHKSVY EFTPCGYPAD DTSSDWKTTH
FEYDALGNTI LKLDILGQDD PTMLKHLGDL THVNPQNIPR FDKKLTEMFW SVNPLKLKPH
YLDEPTGAIG IPEFGTKFVR KILEQTKPKG FGDLIRVSGL SHGKNVWADN AQKILKDQNL
SLKDVIACRD DIMLYLIHKG MQAKDAFEIM EKVRKGIALN AKEVQLMQSN GVEQHWINSC
LKISYLFPKA HAAAYVLMAW RIAWFKLYHP LSYYACLLSF KLKEHDVSGF KSGVSFVKQK
LEELNTLYRI KRIKPKEAEL LTSYEVYLEM MARGIKLEQI SLTHSHATRF VEHNGMLIAP
FITIPGMGEA VANSIIEARN EKPFSSLDDF KKRTKITKKH IEAFTQMQLL DEFREQDNQK
KLF
