DPO3_OCEIH
ID DPO3_OCEIH Reviewed; 1428 AA.
AC Q8EQU6;
DT 03-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=OB1593;
OS Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC
OS 3954 / HTE831).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Oceanobacillus.
OX NCBI_TaxID=221109;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831;
RX PubMed=12235376; DOI=10.1093/nar/gkf526;
RA Takami H., Takaki Y., Uchiyama I.;
RT "Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge
RT and its unexpected adaptive capabilities to extreme environments.";
RL Nucleic Acids Res. 30:3927-3935(2002).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR EMBL; BA000028; BAC13549.1; -; Genomic_DNA.
DR RefSeq; WP_011065993.1; NC_004193.1.
DR AlphaFoldDB; Q8EQU6; -.
DR SMR; Q8EQU6; -.
DR STRING; 221109.22777276; -.
DR PRIDE; Q8EQU6; -.
DR EnsemblBacteria; BAC13549; BAC13549; BAC13549.
DR KEGG; oih:OB1593; -.
DR eggNOG; COG2176; Bacteria.
DR HOGENOM; CLU_003297_2_0_9; -.
DR OMA; YYAAYFT; -.
DR OrthoDB; 561611at2; -.
DR PhylomeDB; Q8EQU6; -.
DR Proteomes; UP000000822; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 2.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR SUPFAM; SSF89550; SSF89550; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..1428
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_0000204585"
FT DOMAIN 414..570
FT /note="Exonuclease"
SQ SEQUENCE 1428 AA; 161813 MW; 73175F6A5EC929F0 CRC64;
MDISKKEKLK LLLEQIRMPD NEIEAHFSNS YLDKVEVHKA DKKWHFYINI DHVLPFQTYQ
LFHHSLKESF ASIAEVSLTL SAENRHCPEG DIQIYWKHFI AQTSLSPAYR DLVLDQAPEV
SNNKIMLTAR NEAEASALKK RLEAKFHDFC LSIGSLPYTL EVEVKTNTEA MQEFRDKKAL
EDQQLVMKTV QEKEKRDKDK SVPDQQPLAI GYKIQDEPIT MDQIIDEERR ITVQGYVFDA
EVRELRSGRS LLIIKATDYT DSIQIKMFSK GDEDAAKFAS VKKGMWVKAR GSIQTDMYTN
ELAMMAKDIH EIHVSQRMDD ASEDKKRVEL HTHTTMSQMD AVVSPEALVE QAAKWGHKAI
AITDHAGVQG FPDAHNAGKK HGVKVLYGVE ANLVDDGVPI AYNEADRNLY EDTFVVFDVE
TTGLSAVYDT IIELAAVKIK GGEIVDRFES FANPHHALSQ TTIDLTGITD DMVNDAPEVA
DVLKDFHTWM GNDILVAHNA SFDMGFLNQG FQRIDYEKAS NPVVDTLELA RFLLPELRNH
RLNTLCKHLD IELTQHHRAI YDAEATGYLL WKLVQRLVEK EISNHLELNN HMGENNAYQR
SRPYHCTLIA QTEEGLKNLY KLVSRAHIDF FYRVPRLPRS VLQKHREGIL VGTACDKGEV
FETMMQKSEE EAEQVADFYD YIEVQPPENY THLIEKDLVQ NESQILDILR KLVNLGDKMK
KTVVATGNVH YLHEHDKQYR QILIGSQAGN PLSRHKLPDT PFRTTTEMIN CFHFLGEKKA
NEIVVDNTQK LTDSIEEISP VKDGLFTPNI EGAEQEMRDL CYNKAKEVYG EPVPQIVVDR
LEKELESIIG NGFAVIYLIS QKLVKKSLDD GYLVGSRGSV GSSFVATMTE ITEVNPLVPH
YVCKHCHHHE FFTDGSVASG FDLPDKDCPG CGKGLTKDGQ DIPFETFLGF KGDKVPDIDL
NFSGDYQPRA HNYTKELFGI DNVYRAGTIG TVAEKTAYGY VKGYASDNQF VYKNAEVDRL
VQGCTGVKRT TGQHPGGIIV VPSDKEIYDF TPIQFPADDR NSEWRTTHFD FHSIHDNLLK
LDILGHDDPT VIRMLQDLSG MDPKNIPTDD EEVMKIFSGT ESLGVTPEQI NCKTGTLGVP
EFGTKFVRQM LEDTKPKTFA ELLIISGLSH GTDVWLGNAQ ELINDGICQL PDVIGCRDDI
MVYLMHKGLD PSMAFTIMEF VRKGKGLKDE WIDEMKKHAV PDWYIESCKK IKYMFPKAHA
AAYVLMAVRI AYFKVHHPIL FYAAYFTVRA DDFELDTMIK GSDAIRKRIE EITVKGNDAS
PKEKNLLTVL EISLEMCERG FSFKKVDLYQ SSATDFIVEG DSLLPPFNAV DGLGTNAALN
IVKAREEGEF LSKEDLRERS KISKTVLEYL DNHGCLEGME DKNQLSLF
