DPO3_PEDPA
ID DPO3_PEDPA Reviewed; 1437 AA.
AC Q03FS8;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2006, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=PEPE_0885;
OS Pediococcus pentosaceus (strain ATCC 25745 / CCUG 21536 / LMG 10740 /
OS 183-1w).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Pediococcus.
OX NCBI_TaxID=278197;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25745 / CCUG 21536 / LMG 10740 / 183-1w;
RX PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT "Comparative genomics of the lactic acid bacteria.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR EMBL; CP000422; ABJ67944.1; -; Genomic_DNA.
DR RefSeq; WP_011673320.1; NC_008525.1.
DR AlphaFoldDB; Q03FS8; -.
DR SMR; Q03FS8; -.
DR STRING; 278197.PEPE_0885; -.
DR EnsemblBacteria; ABJ67944; ABJ67944; PEPE_0885.
DR GeneID; 33062949; -.
DR KEGG; ppe:PEPE_0885; -.
DR eggNOG; COG2176; Bacteria.
DR HOGENOM; CLU_003297_0_0_9; -.
DR OMA; YYAAYFT; -.
DR OrthoDB; 561611at2; -.
DR Proteomes; UP000000773; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..1437
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_1000048477"
FT DOMAIN 420..576
FT /note="Exonuclease"
SQ SEQUENCE 1437 AA; 161831 MW; 649F98298D7706B1 CRC64;
MSLSENEQFR VLLEQIDLLD NAELKDGSIK DLTVHSNSKR WTFSFEFNDI LPLQIFVEFH
HQLVNTFGSI AQIDFSIDTI NKNNVATHAT DYWDWIIKNS GIQSSLLQVL CDKSIPEFKD
GQLVVKVDNQ ITKNFLNDDV LQTLSNTYQK VGFGPIKIKP TIDDNTAQER LQAYHEEKIK
RDQAIVQKAT EAIKKAESKK KASDKAMPEF NGKVQMGKPI NGKDNIVQLR DITQEERSVS
IEGYIFDAEV KTLRSERQLL IFKVTDYTSS ITIKKFSRNE ADEQLFSAIK KGLWVRVRGS
IQEDSFMKDL TMNAYDIMEV DHADRQDTAT DKRVELHLHT NMSTMDATNG ISDLVGQAAK
WGHPAVAVTD HAGLQAFPEA HAAGKKSGIK TLFGVEANLV DDGVPIGYNS EHCPLKGGKY
VIFDVETTGL SAIYDKVIEL SAVKMENGNV IDQFEEFIDP GFPLSETTIN LTSITDEMVR
GSMSEKEVFT KFREFYEDAI IVGHNVTFDI GFMNTGYLRH GMEEIKNPII DTLTLARFLY
PTLKGYRLNT LAKKFGVALE HHHRAIYDSE TTGHLCYLFL KDAEERYGIE YHDQLNDHMT
ENEAYKHARP SHAILIAKTQ AGLKNLFKLV SMSNVKYYYR VPRIPRSELE KLREGIIVGS
ACSSGDVFTA MMQKGYADAR RKAKFYDYLE VMPKPVYQPL IEQELVSDNK KLEEIIANIV
KLGEDLDIPV VATGDVHYLN PQDYIYRKIL INSQGGANPL NRSELPDVHF RTTDEMLEDF
SFLGPEVAHK IVVENSNKIA DSVDDGISPV KDKLYTPKMP GAEDRIKQLS MDRAHALYGE
VLPEIVQERL DKELTSIIGN GFSVIYLIAQ RLVHKSNKDG YLVGSRGSVG SSFVATMSGI
TEVNPLPPHY RCPKCQYSFF YTKGEYGSGY DLPDKECPEC GTWMIGDGQD IPFETFLGFK
GDKVPDIDLN FSGDYQPIAH NYMQVLFGKD NVFRAGTIGT VADKTAFGYV KAYERDTNQH
LRGAEIERLA KGATGVKRTT GQHPAGILIV PDYMDVYDFS PIQYPADDQT AAWETTHFDF
HSIHDNILKM DILGHDDPTM IRKLQDLSGI DPETIPTDDP GVMKIFSGPE VLGVDENQIQ
SKTGTLGIPE FGTRFVRGML EETHPTSYSE LLQISGLSHG TDVWLGNAEE LIKAGTVTLK
EVIGCRDNIM MDLIHYGLDS QSSFQIMESV RKGKGIPEGY EDKMRAANVP DWYIESCGKI
KYMFPRAHAS AYVLMALRIA YFKVYYPLVY YAAYFTVRAD DFDIVSMARG KESLKNRMQE
INDKGNDAST KEKNLLTVLE LANEMLERGF EFKMIDIEKS DAEEWLIEGK SLIAPFNALP
GLGLNVAKQI VAARADKPFL SKEDLSKRGH VSQTLIDFMT ENNVLSELPD ENQLSLF
