ID   DPO3_STAA3              Reviewed;        1436 AA.
AC   Q2FHH4;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE            Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN   Name=polC {ECO:0000255|HAMAP-Rule:MF_00356};
GN   OrderedLocusNames=SAUSA300_1157;
OS   Staphylococcus aureus (strain USA300).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=367830;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=USA300;
RX   PubMed=16517273; DOI=10.1016/s0140-6736(06)68231-7;
RA   Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G.,
RA   Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F.,
RA   Perdreau-Remington F.;
RT   "Complete genome sequence of USA300, an epidemic clone of community-
RT   acquired meticillin-resistant Staphylococcus aureus.";
RL   Lancet 367:731-739(2006).
CC   -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC       also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC       Rule:MF_00356}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00356};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR   EMBL; CP000255; ABD22133.1; -; Genomic_DNA.
DR   RefSeq; WP_001820550.1; NZ_CP027476.1.
DR   AlphaFoldDB; Q2FHH4; -.
DR   SMR; Q2FHH4; -.
DR   PRIDE; Q2FHH4; -.
DR   EnsemblBacteria; ABD22133; ABD22133; SAUSA300_1157.
DR   KEGG; saa:SAUSA300_1157; -.
DR   HOGENOM; CLU_003297_2_0_9; -.
DR   OMA; YYAAYFT; -.
DR   Proteomes; UP000001939; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.150.700; -; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   HAMAP; MF_00356; DNApol_PolC; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR024754; DNA_PolC-like_N_II.
DR   InterPro; IPR028112; DNA_PolC-type_N_I.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR006054; DnaQ.
DR   InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR006308; PolC_gram_pos.
DR   InterPro; IPR044923; PolC_middle_finger_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF14480; DNA_pol3_a_NI; 1.
DR   Pfam; PF11490; DNA_pol3_a_NII; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 2.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF00929; RNase_T; 1.
DR   SMART; SM00479; EXOIII; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   TIGRFAMs; TIGR00573; dnaq; 1.
DR   TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW   Hydrolase; Nuclease; Nucleotidyltransferase; Transferase.
FT   CHAIN           1..1436
FT                   /note="DNA polymerase III PolC-type"
FT                   /id="PRO_1000048479"
FT   DOMAIN          420..576
FT                   /note="Exonuclease"
SQ   SEQUENCE   1436 AA;  162460 MW;  2F70E034C0FBF723 CRC64;
     MTEQQKFKVL ADQIKISNQL DAEILNSGEL TRIDVSNKNR TWEFHITLPQ FLAHEDYLLF
     INAIEQEFKD IANVTCRFTV TNGTNQDEHA IKYFGHCIDQ TALSPKVKGQ LKQKKLIMSG
     KVLKVMVSND IERNHFDKAC NGSLIKAFRN CGFDIDKIIF ETNDNDQEQN LASLEAHIQE
     EDEQSARLAT EKLEKMKAEK AKQQDNNESA VDKCQIGKPI QIENIKPIES IIEEEFKVAI
     EGVIFDINLK ELKSGRHIVE IKVTDYTDSL VLKMFTRKNK DDLEHFKALS VGKWVRAQGR
     IEEDTFIRDL VMMMSDIEEI KKATKKDKAE EKRVEFHLHT AMSQMDGIPN IGAYVKQAAD
     WGHPAIAVTD HNVVQAFPDA HAAAEKHGIK MIYGMEGMLV DDGVPIAYKP QDVVLKDATY
     VVFDVETTGL SNQYDKIIEL AAVKVHNGEI IDKFERFSNP HERLSETIIN LTHITDDMLV
     DAPEIEEVLT EFKEWVGDAI FVAHNASFDM GFIDTGYERL GFGPSTNGVI DTLELSRTIN
     TEYGKHGLNF LAKKYGVELT QHHRAIYDTE ATAYIFIKMV QQMKELGVLN HNEINKKLSN
     EDAYKRARPS HVTLIVQNQQ GLKNLFKIVS ASLVKYFYRT PRIPRSLLDE YREGLLVGTA
     CDEGELFTAV MQKDQSQVEK IAKYYDFIEI QPPALYQDLI DRELIRDTET LHEIYQRLIH
     AGDTAGIPVI ATGNAHYLFE HDGIARKILI ASQPGNPLNR STLPEAHFRT TDEMLNEFHF
     LGEEKAHEIV VKNTNELADR IERVVPIKDE LYTPRMEGAN EEIRELSYAN ARKLYGEDLP
     QIVIDRLEKE LKSIIGNGFA VIYLISQRLV KKSLDDGYLV GSRGSVGSSF VATMTEITEV
     NPLPPHYICP NCKTSEFFND GSVGSGFDLP DKTCETCGAP LIKEGQDIPF ETFLGFKGDK
     VPDIDLNFSG EYQPNAHNYT KVLFGEDKVF RAGTIGTVAE KTAFGYVKGY LNDQGIHKRG
     AEIDRLVKGC TGVKRTTGQH PGGIIVVPDY MDIYDFTPIQ YPADDQNSAW MTTHFDFHSI
     HDNVLKLDIL GHDDPTMIRM LQDLSGIDPK TIPVDDKEVM QIFSTPESLG VTEDEILCKT
     GTFGVPEFGT GFVRQMLEDT KPTTFSELVQ ISGLSHGTDV WLGNAQELIK TGICDLSSVI
     GCRDDIMVYL MYAGLEPSMA FKIMESVRKG KGLTEEMIET MKENEVPDWY LDSCLKIKYM
     FPKAHAAAYV LMAVRIAYFK VHHPLYYYAS YFTIRASDFD LITMIKDKTS IRNTVKDMYS
     RYMDLGKKEK DVLTVLEIMN EMAHRGYRMQ PISLEKSQAF EFIIEGDTLI PPFISVPGLG
     ENVAKRIVEA RDDGPFLSKE DLNKKAGLSQ KIIEYLDELG SLPNLPDKAQ LSIFDM