ID   DPO3_STAAU              Reviewed;        1435 AA.
AC   P0C1P9; P68851; Q53665; Q57110; Q9F1J9;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE            Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN   Name=polC {ECO:0000255|HAMAP-Rule:MF_00356};
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=IP8;
RX   PubMed=7489915; DOI=10.1016/0378-1119(95)00377-i;
RA   Pacitti D.F., Barnes M.H., Li D., Brown N.C.;
RT   "Characterization and overexpression of the gene encoding Staphylococcus
RT   aureus DNA polymerase III.";
RL   Gene 165:51-56(1995).
CC   -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC       also exhibits 3' to 5' exonuclease activity.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00356};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR   EMBL; D86727; BAA13160.1; -; Genomic_DNA.
DR   EMBL; Z48003; CAA88043.1; -; Genomic_DNA.
DR   PIR; S52267; S52267.
DR   AlphaFoldDB; P0C1P9; -.
DR   SMR; P0C1P9; -.
DR   BindingDB; P0C1P9; -.
DR   ChEMBL; CHEMBL4910; -.
DR   PRIDE; P0C1P9; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.150.700; -; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   HAMAP; MF_00356; DNApol_PolC; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR024754; DNA_PolC-like_N_II.
DR   InterPro; IPR028112; DNA_PolC-type_N_I.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR006054; DnaQ.
DR   InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR006308; PolC_gram_pos.
DR   InterPro; IPR044923; PolC_middle_finger_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF14480; DNA_pol3_a_NI; 1.
DR   Pfam; PF11490; DNA_pol3_a_NII; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 2.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF00929; RNase_T; 1.
DR   SMART; SM00479; EXOIII; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   TIGRFAMs; TIGR00573; dnaq; 1.
DR   TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW   Hydrolase; Nuclease; Nucleotidyltransferase; Transferase.
FT   CHAIN           1..1435
FT                   /note="DNA polymerase III PolC-type"
FT                   /id="PRO_0000204591"
FT   DOMAIN          420..576
FT                   /note="Exonuclease"
SQ   SEQUENCE   1435 AA;  162477 MW;  59CA6C672B56981F CRC64;
     MTEQQKFKVL ADQIKISNQL DAEILNSGEL TRIDVSNKNR TWEFHITLPQ FLAHEDYLLF
     INAIEQEFKD IANVTCRFTV TNGTNQDEHA IKYFGHCIDQ TALSPKVKGQ LKQKKLIMSG
     KVLKVMVSND IERNHFDKAC NGSLIKAFRN CGFDIDKIIF ETNDNDQEQN LASLEAHIQE
     EDEQSARLAT EKLEKMKAEK AKQQDNKQSA VDKCQIGKPI QIENIKPIES IIEEEFKVAI
     EGVIFDINLK ELKSGRHIVE IKVTDYTDSL VLKMFTRKNK DDLEHFKALS VGKWVRAQGR
     IEEDTFIRDL VMMMSDIEEI KKATKKDKAE EKRVEFHLHT AMSQMDGIPN IGAYVKQAAD
     WGHPAIAVTD HNVVQAFPDA HAAAEKHGIK MIYGMEGMLV DDGVPIAYKP QDVVLKDATY
     VVFDVETTGL SNQYDKIIEL AAVKVHNGEI IDKFERFSNP HERLSETIIN LTHITDDMLV
     DAPEIEEVLT EFKEWVGDAI FVAHNASFDM GFIDTGYERL GFGPSTNGVI DTLELSRTIN
     TEYGKHGLNF LAKKYGVELT QHHRAIYDTE ATAYIFIKMV QQMKELGVLN HNEINKKLSN
     EDAYKRARPS HVTLIVQNQQ GLKNLFKIVS ASLVKYFYRT PRIPRSLLDE YREGLLVGTA
     CDEGELFTAV MQKDQSQVEK IAKYYDFIEI QPPALYQDLI DRELIRDTET LHEIYQRLIH
     AGDTAGIPVI ATGNAHYLFE HDGIARKILI ASQPGNPLNR STLPEAHFRT TDEMLNEFHF
     LGEEKAHEIV VKNTNELADR IERVVPIKDE LYTPRMEGAN EEIRELSYAN ARKLYGEDLP
     QIVIDRLEKE LKSIIGNGFA VIYLISQRLV KKSLDDGYLV GSRGSVGSSF VATMTEITEV
     NPLPPHYICP NCKTSEFFND GSVGSGFDLP DKTCETCGAP LIKEGQDIPF EKFLGFKGDK
     VPDIDLNFSG EYQPNAHNYT KVLFGEDKVF RAGTIGTVAE KTAFGYVKGY LNDQGIHKRG
     AEIDRLVKGC TGVKATTGQH PGGIIVVPDY MDIYDFTPIQ YPADDQNSAW MTTHFDFHSI
     HDNVLKLDIL GHDDPTMIRM LQDLSGIDPK TIPVDDKEVM QIFSTPESLG VTEDEILCKT
     GTFGVPNSDR IRRQMLEDTK PTTFSELVQI SGLSHGTDVW LGNAQELIKT GICDLSSVIG
     CRDDIMVYLM YAGLEPSMAF KIMESVRKGK GLTEEMIETM KENEVPDWYL DSCLKIKYIF
     PKAHAAAYVL MAVRIAYFKV HHPLYYYASY FTIRASDFDL ITMIKDKTSI RNTVKDMYSR
     YMDLGKKEKD VLTVLEIMNE MAHRGYRMQP ISLEKSQAFE FIIEGDTLIP PFISVPGLGE
     NVAKRIVEAR DDGPFLSKED LNKKAGLYQK IIEYLDELGS LPNLPDKAQL SIFDM