DPO3_STAAW
ID DPO3_STAAW Reviewed; 1436 AA.
AC P68852; Q53665; Q57110; Q9F1J9;
DT 21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=MW1147;
OS Staphylococcus aureus (strain MW2).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=196620;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MW2;
RX PubMed=12044378; DOI=10.1016/s0140-6736(02)08713-5;
RA Baba T., Takeuchi F., Kuroda M., Yuzawa H., Aoki K., Oguchi A., Nagai Y.,
RA Iwama N., Asano K., Naimi T., Kuroda H., Cui L., Yamamoto K., Hiramatsu K.;
RT "Genome and virulence determinants of high virulence community-acquired
RT MRSA.";
RL Lancet 359:1819-1827(2002).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB95012.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; BA000033; BAB95012.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_001820550.1; NC_003923.1.
DR AlphaFoldDB; P68852; -.
DR SMR; P68852; -.
DR EnsemblBacteria; BAB95012; BAB95012; BAB95012.
DR KEGG; sam:MW1147; -.
DR HOGENOM; CLU_003297_2_0_9; -.
DR Proteomes; UP000000418; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 2.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Transferase.
FT CHAIN 1..1436
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_0000204592"
FT DOMAIN 420..576
FT /note="Exonuclease"
SQ SEQUENCE 1436 AA; 162460 MW; 2F70E034C0FBF723 CRC64;
MTEQQKFKVL ADQIKISNQL DAEILNSGEL TRIDVSNKNR TWEFHITLPQ FLAHEDYLLF
INAIEQEFKD IANVTCRFTV TNGTNQDEHA IKYFGHCIDQ TALSPKVKGQ LKQKKLIMSG
KVLKVMVSND IERNHFDKAC NGSLIKAFRN CGFDIDKIIF ETNDNDQEQN LASLEAHIQE
EDEQSARLAT EKLEKMKAEK AKQQDNNESA VDKCQIGKPI QIENIKPIES IIEEEFKVAI
EGVIFDINLK ELKSGRHIVE IKVTDYTDSL VLKMFTRKNK DDLEHFKALS VGKWVRAQGR
IEEDTFIRDL VMMMSDIEEI KKATKKDKAE EKRVEFHLHT AMSQMDGIPN IGAYVKQAAD
WGHPAIAVTD HNVVQAFPDA HAAAEKHGIK MIYGMEGMLV DDGVPIAYKP QDVVLKDATY
VVFDVETTGL SNQYDKIIEL AAVKVHNGEI IDKFERFSNP HERLSETIIN LTHITDDMLV
DAPEIEEVLT EFKEWVGDAI FVAHNASFDM GFIDTGYERL GFGPSTNGVI DTLELSRTIN
TEYGKHGLNF LAKKYGVELT QHHRAIYDTE ATAYIFIKMV QQMKELGVLN HNEINKKLSN
EDAYKRARPS HVTLIVQNQQ GLKNLFKIVS ASLVKYFYRT PRIPRSLLDE YREGLLVGTA
CDEGELFTAV MQKDQSQVEK IAKYYDFIEI QPPALYQDLI DRELIRDTET LHEIYQRLIH
AGDTAGIPVI ATGNAHYLFE HDGIARKILI ASQPGNPLNR STLPEAHFRT TDEMLNEFHF
LGEEKAHEIV VKNTNELADR IERVVPIKDE LYTPRMEGAN EEIRELSYAN ARKLYGEDLP
QIVIDRLEKE LKSIIGNGFA VIYLISQRLV KKSLDDGYLV GSRGSVGSSF VATMTEITEV
NPLPPHYICP NCKTSEFFND GSVGSGFDLP DKTCETCGAP LIKEGQDIPF ETFLGFKGDK
VPDIDLNFSG EYQPNAHNYT KVLFGEDKVF RAGTIGTVAE KTAFGYVKGY LNDQGIHKRG
AEIDRLVKGC TGVKRTTGQH PGGIIVVPDY MDIYDFTPIQ YPADDQNSAW MTTHFDFHSI
HDNVLKLDIL GHDDPTMIRM LQDLSGIDPK TIPVDDKEVM QIFSTPESLG VTEDEILCKT
GTFGVPEFGT GFVRQMLEDT KPTTFSELVQ ISGLSHGTDV WLGNAQELIK TGICDLSSVI
GCRDDIMVYL MYAGLEPSMA FKIMESVRKG KGLTEEMIET MKENEVPDWY LDSCLKIKYM
FPKAHAAAYV LMAVRIAYFK VHHPLYYYAS YFTIRASDFD LITMIKDKTS IRNTVKDMYS
RYMDLGKKEK DVLTVLEIMN EMAHRGYRMQ PISLEKSQAF EFIIEGDTLI PPFISVPGLG
ENVAKRIVEA RDDGPFLSKE DLNKKAGLSQ KIIEYLDELG SLPNLPDKAQ LSIFDM