DPO3_STAEQ
ID DPO3_STAEQ Reviewed; 1438 AA.
AC Q5HPS7;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 2.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=SERP0831;
OS Staphylococcus epidermidis (strain ATCC 35984 / RP62A).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=176279;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35984 / RP62A;
RX PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA Fraser C.M.;
RT "Insights on evolution of virulence and resistance from the complete genome
RT analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT strain.";
RL J. Bacteriol. 187:2426-2438(2005).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAW54192.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000029; AAW54192.1; ALT_INIT; Genomic_DNA.
DR AlphaFoldDB; Q5HPS7; -.
DR SMR; Q5HPS7; -.
DR STRING; 176279.SERP0831; -.
DR EnsemblBacteria; AAW54192; AAW54192; SERP0831.
DR KEGG; ser:SERP0831; -.
DR eggNOG; COG2176; Bacteria.
DR HOGENOM; CLU_003297_0_0_9; -.
DR OrthoDB; 561611at2; -.
DR Proteomes; UP000000531; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..1438
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_0000204594"
FT DOMAIN 422..578
FT /note="Exonuclease"
SQ SEQUENCE 1438 AA; 162983 MW; 10DDA130BFCFC873 CRC64;
MAMTNREKFK VLADQIKISN QLEQDILEQG ELTRIDVSNK NRTWTFQISL PHFLSHEDYL
LFTHAIEEEF KEIATVAIDF SIKDTNNQDE FALKYFGHCI DQTRLSPKVK GQLKQKKLIM
SGNVLKVLVS NDIERNHFDK ACNGSLVKAF RQCGFEIDKV VFETDSTNHD DDLASLEAHI
QQEDEQSARE ATEKLEKMKA EKAKQQDNNE STVEKCQIGK PIQIENIKPI ESIIEEEFKV
AIEGVIFDIN LKELKSGRHI VELKVTDYTD SLVLKMFTRK NKDDLDHFKA LSVGKWVRAQ
GRIEEDTFVR DLVMMMSDIE EIKKTPKQDK AEDKRVEFHL HTSMSQMDGI PNISAYVEQA
AKWGHQALAV TDHNVVQAFP DAHNAAEKHG IKMIYGMEGM LVDDGVPIAY KPTDRNLKDA
TYVVFDVETT GLSNQYDKII ELAAVKVHNG EIIDKFERFS NPHERLSETI INLTHITDDM
LTDAPEIEEV LTEFKEWVGD AIFVAHNASF DMGFIDTGYE RLGFGPSTNG VIDTLELSRT
INTEYGKHGL NFLAKKYGVE LTQHHRAIYD TEATAYIFIK MVQQMKELGV NNHLEINKKL
TNEDAYKRAR PSHVTLIVQN QEGLKNLFKI VSASLVKYYY RTPRIPRSLL NEYREGILIG
TACDEGELFT AVMQKDQSEV EKIAKFYDFI EVQPPALYQD LMDRELIRDN ETLTQIYKRL
IDAGKSANIP VIATGNAHYL YEHDAIARKI LIASQPGNPL NRSTLPEAHF RTTDEMLDDF
HFLGEEKAYE IVVTNTNELA NKIEKVVPIK DKLFTPRMDG ANEEIRELSY SNAKKLYGED
LPQIVIDRLE KELDSIIGNG FSVIYLISQR LVKKSLDDGY LVGSRGSVGS SFVATMTEIT
EVNPLPPHYI CSHCKTSEFF DDGSVGSGFD LPDKKCPTCG NELIKEGQDI PFETFLGFKG
DKVPDIDLNF SGEYQPNAHN YTKVLFGEDK VFRAGTIGTV AEKTAFGFVK GYLNDQGIHK
RGAEIDRLVK GCTGVKRTTG QHPGGIIVVP DYMDIYDFTP IQFPADDQSA AWMTTHFDFH
SIHDNVLKLD ILGHDDPTMI RMLQDLSGID PKTIPVDDKE TMQIFSGPES LGVTEDEILC
KTGTFGVPEF GTGFVRQMLE DTKPTTFSEL VQISGLSHGT DVWLGNAQEL IRQGICDLSS
VIGCRDDIMV YLMYAGLEPS MAFKTMEFVR KGRGLTDEMV EAMKENNVPD WYLDSCRKIK
YMFPKAHAAA YVLMAVRIAY FKVHHPLYYY AAYFTIRASD FDLITMIKDK TSIRNTVKDM
YSRYMDLGKK EKDVLTVLEI MNEMAHRGFR LQPISLEKSQ AFDFIIEGDT LIPPFISVPG
LGENVAQRIV EAREEGPFLS KEDLNKKAGL SQKVIDYLDE LGSLPDLPDK AQLSIFDM
