DPO3_STAES
ID DPO3_STAES Reviewed; 1442 AA.
AC Q8CPG6;
DT 03-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=SE_0940;
OS Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=176280;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 12228 / FDA PCI 1200;
RX PubMed=12950922; DOI=10.1046/j.1365-2958.2003.03671.x;
RA Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q.,
RA Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H.,
RA Zhao G.-P., Qu D., Danchin A., Wen Y.-M.;
RT "Genome-based analysis of virulence genes in a non-biofilm-forming
RT Staphylococcus epidermidis strain (ATCC 12228).";
RL Mol. Microbiol. 49:1577-1593(2003).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR EMBL; AE015929; AAO04537.1; -; Genomic_DNA.
DR RefSeq; NP_764495.1; NC_004461.1.
DR AlphaFoldDB; Q8CPG6; -.
DR SMR; Q8CPG6; -.
DR STRING; 176280.SE_0940; -.
DR EnsemblBacteria; AAO04537; AAO04537; SE_0940.
DR KEGG; sep:SE_0940; -.
DR PATRIC; fig|176280.10.peg.915; -.
DR eggNOG; COG2176; Bacteria.
DR HOGENOM; CLU_003297_0_0_9; -.
DR OMA; YYAAYFT; -.
DR Proteomes; UP000001411; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Transferase.
FT CHAIN 1..1442
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_0000204593"
FT DOMAIN 426..582
FT /note="Exonuclease"
SQ SEQUENCE 1442 AA; 163421 MW; 05268D58C0722938 CRC64;
MVIILAMTNR EKFKVLADQI KISNQLEQDI LEQGELTRID VSNKNRTWTF QISLPHFLSH
EDYLLFTHAI EEEFKEIATV AIDFSIKDTN NQDEFALKYF GHCIDQTRLS PKVKGQLKQK
KLIMSGNVLK VLVSNDIERN HFDKACNGSL VKAFRQCGFE IDKVVFETDS TNHDDDLASL
EAHIQQEDEQ SAREATEKLE KMKAEKAKQQ DNNESTVEKC QIGKPIQIEN IKPIESIIEE
EFKVAIEGVI FDINLKELKS GRHIVELKVT DYTDSLVLKM FTRKNKDDLD HFKALSVGKW
VRAQGRIEED TFVRDLVMMM SDIEEIKKTP KQDKAEDKRV EFHLHTSMSQ MDGIPNISAY
VEQAAKWGHQ ALAVTDHNVV QAFPDAHNAA EKHGIKMIYG MEGMLVDDGV PIAYKPTDRN
LKDATYVVFD VETTGLSNQY DKIIELAAVK VHNGEIIDKF ERFSNPHERL SETIINLTHI
TDDMLTDAPE IEEVLTEFKE WVGDAIFVAH NASFDMGFID TGYERLGFGP STNGVIDTLE
LSRTINTEYG KHGLNFLAKK YGVELTQHHR AIYDTEATAY IFIKMVQQMK ELGVNNHLEI
NKKLTNEDAY KRARPSHVTL IVQNQEGLKN LFKIVSASLV KYYYRTPRIP RSLLNEYREG
ILIGTACDEG ELFTAVMQKD QSEVEKIAKF YDFIEVQPPA LYQDLMDREL IRDNETLTQI
YKRLIDAGKS ANIPVIATGN AHYLYEHDAI ARKILIASQP GNPLNRSTLP EAHFRTTDEM
LDDFHFLGEE KAYEIVVTNT NELANKIEKV VPIKDKLFTP RMDGANEEIR ELSYSNAKKL
YGEDLPQIVI DRLEKELDSI IGNGFSVIYL ISQRLVKKSL DDGYLVGSRG SVGSSFVATM
TEITEVNPLP PHYICSHCKT SEFFDDGSVG SGFDLPDKKC PTCGNELIKE GQDIPFETFL
GFKGDKVPDI DLNFSGEYQP NAHNYTKVLF GEDKVFRAGT IGTVAEKTAF GFVKGYLNDQ
GIHKRGAEID RLVKGCTGVK RTTGQHPGGI IVVPDYMDIY DFTPIQFPAD DQSAAWMTTH
FDFHSIHDNV LKLDILGHDD PTMIRMLQDL SGIDPKTIPV DDKETMQIFS GPESLGVTED
EILCKTGTFG VPEFGTGFVR QMLEDTKPTT FSELVQISGL SHGTDVWLGN AQELIRQGIC
DLSSVIGCRD DIMVYLMYAG LEPSMAFKTM EFVRKGRGLT DEMVEAMKEN NVPDWYLDSC
RKIKYMFPKA HAAAYVLMAV RIAYFKVHHP LYYYAAYFTI RASDFDLITM IKDKTSIRNT
VKDMYSRYMD LGKKEKDVLT VLEIMNEMAH RGFRLQPISL EKSQAFDFII EGDTLIPPFI
SVPGLGENVA QRIVEAREEG PFLSKEDLNK KAGLSQKVID YLDELGSLPD LPDKAQLSIF
DM
