DPO3_STRA5
ID DPO3_STRA5 Reviewed; 1468 AA.
AC P63984; Q8CX24; Q8DXE0;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=SAG1911;
OS Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=208435;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-611 / 2603 V/R;
RX PubMed=12200547; DOI=10.1073/pnas.182380799;
RA Tettelin H., Masignani V., Cieslewicz M.J., Eisen J.A., Peterson S.N.,
RA Wessels M.R., Paulsen I.T., Nelson K.E., Margarit I., Read T.D.,
RA Madoff L.C., Wolf A.M., Beanan M.J., Brinkac L.M., Daugherty S.C.,
RA DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Lewis M.R., Radune D.,
RA Fedorova N.B., Scanlan D., Khouri H.M., Mulligan S., Carty H.A.,
RA Cline R.T., Van Aken S.E., Gill J., Scarselli M., Mora M., Iacobini E.T.,
RA Brettoni C., Galli G., Mariani M., Vegni F., Maione D., Rinaudo D.,
RA Rappuoli R., Telford J.L., Kasper D.L., Grandi G., Fraser C.M.;
RT "Complete genome sequence and comparative genomic analysis of an emerging
RT human pathogen, serotype V Streptococcus agalactiae.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:12391-12396(2002).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
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DR EMBL; AE009948; AAN00773.1; -; Genomic_DNA.
DR RefSeq; NP_688900.1; NC_004116.1.
DR RefSeq; WP_001292137.1; NC_004116.1.
DR AlphaFoldDB; P63984; -.
DR SMR; P63984; -.
DR STRING; 208435.SAG1911; -.
DR EnsemblBacteria; AAN00773; AAN00773; SAG1911.
DR KEGG; sag:SAG1911; -.
DR PATRIC; fig|208435.3.peg.1915; -.
DR HOGENOM; CLU_003297_2_0_9; -.
DR OMA; YYAAYFT; -.
DR Proteomes; UP000000821; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 2.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..1468
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_0000204596"
FT DOMAIN 430..586
FT /note="Exonuclease"
FT REGION 197..217
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1468 AA; 166120 MW; 0FFFD8BCC2989649 CRC64;
MSELFKKLMD QIEMPLEIKN SSVFSSADII EVKVHSLSRL WEFHFSFPEL LPIEVYRELQ
TRLVNSFEKA DIKATFDIRA ETIDFSDDLL QDYYQQAFCE PLCNSASFKS SFSQLKVHYN
GSQMIISAPQ FVNNNHFRQN HLPRLEQQFS LFGFGKLAID MVSDEQMTQD LKSSFETNRE
QLLEKANQEA MQALEAQKSL EDSAPPSEEV TPTQNYDFKE RIKQRQAGFE KAEITPMIEV
TTEENRIVFE GMVFSVERKT TRTGRHIINF KMTDYTSSFA MQKWAKDDEE LKKYDMISKG
SWLRVRGNIE NNNFTKSLTM NVQDIKEIVH HERKDLMPAD QKRVEFHAHT NMSTMDALPT
VESLIDTAAK WGHPAIAITD HANVQSFPHG YHRAKKAGIK AIFGLEANIV EDKVPISYNE
VDMNLHEATY VVFDVETTGL SAANNDLIQI AASKMFKGNI IEQFDEFIDP GHPLSAFTTE
LTGITDNHVR GSKPILQVLQ EFQNFCQGTV LVAHNATFDV GFMNANYERH NLPLITQPVI
DTLEFARNLY PEYKRHGLGP LTKRFQVALE HHHMANYDAE ATGRLLFIFL KEARENRDVT
NLMELNTKLV AEDSYKKARI KHATIYVQNQ VGLKNIFKLV SLSNVKYFEG VARIPRSVLD
AHREGLLLGT ACSDGEVFDA LLSNGIDAAV TLAKYYDFIE VMPPAIYRPL VVRDLIKDEV
GIQQIIRDLI EVGRRLDKPV LATGNVHYIE PEDEIYREII VRSLGQGAMI NRTIGRGEDA
QPAPLPKAHF RTTNEMLDEF AFLGKDLAYE IVVTNTNTFA DRFEDVEVVK GDLYTPFVDR
AEERVAELTY AKAFEIYGNP LPDIIDLRIE KELASILGNG FAVIYLASQM LVQRSNERGY
LVGSRGSVGS SFVATMIGIT EVNPMPPHYV CPNCQHSEFI TDGSCGSGYD LPNKNCPKCG
TLYKKDGQDI PFETFLGFDG DKVPDIDLNF SGDDQPSAHL DVRDIFGEEY AFRAGTVGTV
AEKTAFGFVK GYERDYNKFY NDAEVERLAT GAAGVKRSTG QHPGGIVVIP NYMDVYDFTP
VQYPADDMTA AWQTTHFNFH DIDENVLKLD ILGHDDPTMI RKLQDLSGID PSNILPDDPD
VMKLFSGTEV LGVTEEQIGT PTGMLGIPEF GTNFVRGMVN ETHPTTFAEL LQLSGLSHGT
DVWLGNAQDL IKEGIATLST VIGCRDDIMV YLMHAGLQPK MAFTIMERVR KGLWLKISED
ERNGYIQAMR DNNVPDWYIE SCGKIKYMFP KAHAAAYVLM ALRVAYFKVH YPIFYYCAYF
SIRAKAFELR TMSAGLDAVK ARMKDITEKR QRNEATNVEN DLFTTLELVN EMLERGFKFG
KLDLYRSHAT DFIIEEDTLI PPFVAMEGLG ENVAKQIVRA REDGEFLSKT ELRKRGGVSS
TLVEKFDEMG ILGNLPEDNQ LSLFDDFF
