DPO3_STRPG
ID DPO3_STRPG Reviewed; 1465 AA.
AC A2RGI3;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000255|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000255|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000255|HAMAP-Rule:MF_00356}; OrderedLocusNames=SpyM51644;
OS Streptococcus pyogenes serotype M5 (strain Manfredo).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=160491;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Manfredo;
RX PubMed=17012393; DOI=10.1128/jb.01227-06;
RA Holden M.T.G., Scott A., Cherevach I., Chillingworth T., Churcher C.,
RA Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K.,
RA Moule S., Mungall K., Quail M.A., Price C., Rabbinowitsch E., Sharp S.,
RA Skelton J., Whitehead S., Barrell B.G., Kehoe M., Parkhill J.;
RT "Complete genome of acute rheumatic fever-associated serotype M5
RT Streptococcus pyogenes strain Manfredo.";
RL J. Bacteriol. 189:1473-1477(2007).
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity. {ECO:0000255|HAMAP-
CC Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00356}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AM295007; CAM30965.1; -; Genomic_DNA.
DR RefSeq; WP_011889165.1; NC_009332.1.
DR AlphaFoldDB; A2RGI3; -.
DR SMR; A2RGI3; -.
DR KEGG; spf:SpyM51644; -.
DR HOGENOM; CLU_003297_2_0_9; -.
DR OMA; YYAAYFT; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.700; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR006308; PolC_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 2.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR SUPFAM; SSF89550; SSF89550; 1.
DR TIGRFAMs; TIGR00573; dnaq; 1.
DR TIGRFAMs; TIGR01405; polC_Gram_pos; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA replication; DNA-directed DNA polymerase; Exonuclease;
KW Hydrolase; Nuclease; Nucleotidyltransferase; Transferase.
FT CHAIN 1..1465
FT /note="DNA polymerase III PolC-type"
FT /id="PRO_1000048488"
FT DOMAIN 427..583
FT /note="Exonuclease"
SQ SEQUENCE 1465 AA; 164739 MW; B07252A9561833A7 CRC64;
MSDLFAKLMD QIEMPLDMRR SSAFSSADII EVKVHSVSRL WEFHFAFAAV LPIATYRELH
DRLIRTFEAA DIKVTFDIQA AQVDYSDDLL QAYYQEAFEH APCNSASFKS SFSKLKVTYE
NDKLIIAAPR FVNNDHFRKN HLPNLVKQLE AFGFGTLTID MVSDQEMTEH LTKDFVSSRQ
ALVKKAVQDN LEAQKSLEAM MPPVEEATPA PKFDYKERAA KRQAGFEKAT ITPMIEIETE
ENRIVFEGMV FDVERKTTRT GRHIINFKMT DYTSSFALQK WAKDDEELRK FDMIAKGAWL
RVQGNIETNP FTKSLTMNVQ QVKEIVHHER KDLMPEGQKR VELHAHTNMS TMDALPTVES
LIDTAAKWGH KAVAITDHAN VQSFPHGYHR ARKAGIKAIF GLEANIVEDK APISYDPVDM
DLHEATYVVF DVETTGLSAM NNDLIQIAAS KMFKGNIVEQ FDEFIDPGHP LSAFTTELTG
ITDKHLQGAK PLVTVLKAFQ DFCKDSILVA HNASFDVGFM NANYERHDLP KITQPVIDTL
EFARNLYPEY KRHGLGPLTK RFQVSLDHHH MANYDAEATG RLLFIFLRDA REKHGIKNLL
QLNTDLVAED SYKKARIKHA TIYVQNQVGL KNMFKLVSLS NIKYFEGVPR IPRTVLDAHR
EGLLLGTACS DGEVFDAVLT KGIDAAVDLA KYYDFIEIMP PAIYQPLVVR ELIKDQAGIE
QVIRDLIEVG KRANKPVLAT GNVHYLEPEE EIYREIIVRS LGQGAMINRT IGRGEGAQPA
PLPKAHFRTT NEMLDEFAFL GKDLAYQVVV ENTQDFADRI EEVEVVKGDL YTPYIDKAEE
TVAELTYQKA FEIYGNPLPD IIDLRIEKEL TSILGNGFAV IYLASQMLVN RSNERGYLVG
SRGSVGSSFV ATMIGITEVN PMPPHYVCPS CQHSEFITDG SVGSGYDLPN KPCPKCGTPY
QKDGQDIPFE TFLGFDGDKV PDIDLNFSGD DQPSAHLDVR DIFGDEYAFR AGTVGTVAEK
TAYGFVKGYE RDYGKFYRDA EVDRLAAGAA GVKRTTGQHP GGIVVIPNYM DVYDFTPVQY
PADDVTASWQ TTHFNFHDID ENVLKLDILG HDDPTMIRKL QDLSGIDPIT IPADDPGVMA
LFSGTEILGV TPEQIGTPTG MLGIPEFGTN FVRGMVNETH PTTFAELLQL SGLSHGTDVW
LGNAQDLIKE GVATLKTVIG CRDDIMVYLM HAGLEPKMAF TIMERVRKGL WLKISEEERN
GYIDAMRENN VPDWYIESCG KIKYMFPKAH AAAYVLMALR VAYFKVHHPI MYYCAYFSIR
AKAFELKTMS DGLDAVKARM EDITIKRKNN EATNVENDLF TTLEIVNEML ERGFKFGKLD
LYKSDAIEFQ IKGDTLIPPF IALEGLGENV AKQIVKARQE GEFLSKMELR KRGGASSTLV
EKMDEMGILG NMPEDNQLSL FDDFF
