ID   DPO5_SCHPO              Reviewed;         959 AA.
AC   O60094; Q9UTU3;
DT   06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 2.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=rDNA transcriptional regulator pol5;
DE   AltName: Full=DNA polymerase V;
DE            Short=POL V;
DE   AltName: Full=DNA polymerase phi;
GN   Name=pol5; ORFNames=SPBC14C8.14c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=12093911; DOI=10.1073/pnas.142277999;
RA   Shimizu K., Kawasaki Y., Hiraga S., Tawaramoto M., Nakashima N., Sugino A.;
RT   "The fifth essential DNA polymerase phi in Saccharomyces cerevisiae is
RT   localized to the nucleolus and plays an important role in synthesis of
RT   rRNA.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:9133-9138(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10407269;
RX   DOI=10.1002/(sici)1097-0061(199907)15:10a<893::aid-yea430>3.0.co;2-s;
RA   Xiang Z., Lyne M.H., Wood V., Rajandream M.A., Barrell B.G., Aves S.J.;
RT   "DNA sequencing and analysis of a 67.4 kb region from the right arm of
RT   Schizosaccharomyces pombe chromosome II reveals 28 open reading frames
RT   including the genes his5, pol5, ppa2, rip1, rpb8 and skb1.";
RL   Yeast 15:893-901(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 613-795.
RC   STRAIN=ATCC 38364 / 968;
RX   PubMed=10759889; DOI=10.1046/j.1365-2443.2000.00317.x;
RA   Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T.,
RA   Hiraoka Y.;
RT   "Large-scale screening of intracellular protein localization in living
RT   fission yeast cells by the use of a GFP-fusion genomic DNA library.";
RL   Genes Cells 5:169-190(2000).
RN   [5]
RP   PROTEIN FAMILY.
RX   PubMed=12695662; DOI=10.4161/cc.2.2.329;
RA   Yang W., Rogozin I.B., Koonin E.V.;
RT   "Yeast POL5 is an evolutionarily conserved regulator of rDNA transcription
RT   unrelated to any known DNA polymerases.";
RL   Cell Cycle 2:120-122(2003).
RN   [6]
RP   FUNCTION, INTERACTION WITH CDC10, AND SUBCELLULAR LOCATION.
RX   PubMed=16816948; DOI=10.1007/s00438-006-0144-6;
RA   Nadeem F.K., Blair D., McInerny C.J.;
RT   "Pol5p, a novel binding partner to Cdc10p in fission yeast involved in rRNA
RT   production.";
RL   Mol. Genet. Genomics 276:391-401(2006).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-742 AND SER-743, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=18257517; DOI=10.1021/pr7006335;
RA   Wilson-Grady J.T., Villen J., Gygi S.P.;
RT   "Phosphoproteome analysis of fission yeast.";
RL   J. Proteome Res. 7:1088-1097(2008).
CC   -!- FUNCTION: Plays an important role in the regulation of rRNA
CC       transcription. Binds to rDNA promoter fragments.
CC       {ECO:0000269|PubMed:16816948}.
CC   -!- SUBUNIT: Interacts with cdc10. {ECO:0000269|PubMed:16816948}.
CC   -!- INTERACTION:
CC       O60094; P01129: cdc10; NbExp=2; IntAct=EBI-1009362, EBI-1009350;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16816948}.
CC   -!- SIMILARITY: Belongs to the MYBBP1A family. {ECO:0000305}.
CC   -!- CAUTION: Was originally thought to belong to the DNA polymerase type-B
CC       family based on conserved motifs (PubMed:12093911). Has later been
CC       shown to be unrelated to B class DNA polymerases (PubMed:12695662).
CC       {ECO:0000305|PubMed:12093911, ECO:0000305|PubMed:12695662}.
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DR   EMBL; AB012696; BAA32046.1; -; mRNA.
DR   EMBL; CU329671; CAA18436.1; -; Genomic_DNA.
DR   EMBL; AB027997; BAA87301.1; -; Genomic_DNA.
DR   PIR; T00246; T00246.
DR   RefSeq; NP_595917.1; NM_001021825.2.
DR   AlphaFoldDB; O60094; -.
DR   BioGRID; 276383; 7.
DR   IntAct; O60094; 1.
DR   STRING; 4896.SPBC14C8.14c.1; -.
DR   iPTMnet; O60094; -.
DR   MaxQB; O60094; -.
DR   PaxDb; O60094; -.
DR   PRIDE; O60094; -.
DR   EnsemblFungi; SPBC14C8.14c.1; SPBC14C8.14c.1:pep; SPBC14C8.14c.
DR   GeneID; 2539834; -.
DR   KEGG; spo:SPBC14C8.14c; -.
DR   PomBase; SPBC14C8.14c; pol5.
DR   VEuPathDB; FungiDB:SPBC14C8.14c; -.
DR   eggNOG; KOG1926; Eukaryota.
DR   HOGENOM; CLU_005212_1_0_1; -.
DR   InParanoid; O60094; -.
DR   OMA; PWVEVMV; -.
DR   PhylomeDB; O60094; -.
DR   PRO; PR:O60094; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005730; C:nucleolus; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; IDA:PomBase.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0000182; F:rDNA binding; IDA:UniProtKB.
DR   GO; GO:0001163; F:RNA polymerase I transcription regulatory region sequence-specific DNA binding; IDA:PomBase.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0006364; P:rRNA processing; ISO:PomBase.
DR   GO; GO:0009303; P:rRNA transcription; IDA:UniProtKB.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR007015; DNA_pol_V/MYBBP1A.
DR   PANTHER; PTHR13213; PTHR13213; 1.
DR   Pfam; PF04931; DNA_pol_phi; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   1: Evidence at protein level;
KW   Nucleus; Phosphoprotein; Reference proteome.
FT   CHAIN           1..959
FT                   /note="rDNA transcriptional regulator pol5"
FT                   /id="PRO_0000046471"
FT   REGION          936..959
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         742
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18257517"
FT   MOD_RES         743
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18257517"
SQ   SEQUENCE   959 AA;  109719 MW;  02B18B86854EA7CA CRC64;
     MATKTQLELF TKLTSNDKAI RLSSAAQLID SLSNEEELKY SLNRLTKGLS SGRESARIGF
     AVALTELLTR TKDIRATHVL DLLVKHNTAS GNLKGQDERD FYFGLLFGLQ SIVYSGILTH
     KESTIEDFQR VVDLLLQLSG KKNWLQDVCF YVIYKLVEQI PEISFSSNAF LAVNKLLQTP
     AVSKSTEGVG LFLCLTRVPD NVKSEEVAMA NWEPAHPLHK SNLVTLSKIM RQADASETGG
     QNSAWKQKIP MVWKYIFEEY QRKTYSGLAP FHDFWAVVVD EGIFSSTSSL ERKFWGFQIM
     ELALDYVSSD NIGDIFSKNF LHCLINHLSD EDRYLYRAAK RVTSKLEKVS KQNPTLVYPI
     AIHLLGERGS LNFDRVTNTK LVEHILPLAD EQGILQLFQL LLSYVKRCPE DIASDTKAVE
     WRRQWATDTM LSILRSKRSI KQEPWVRELL EIFIAYGYFE VPESEEVIPK FSEGTQNMFR
     LRLMSALSYL SSSAFQQSQT DHQLGDKNWP YVALNYLLEL EKSPKNNLLI SMDESVIEIV
     QKSLSVLHKV TKKIDKKAQH LQQLNAFQLL YSLVLLQVYA GDTDSIDVLE DIDNCYSKVF
     NKKSKRESTS NEPTAMEILT EVMLSLLSRP SLLLRKLVDM LFTSFSEDMN RESIHLICDV
     LKAKESVKDS EGMFAGEEVE EDAFGETEMD EDDFEEIDTD EIEEQSDWEM ISNQDASDNE
     ELERKLDKVL EDADAKVKDE ESSEEELMND EQMLALDEKL AEVFRERKKA SNKEKKKNAQ
     ETKQQIVQFK VKVIDLIDNY YKTQPNNGLG FEFLIPLLEM ILKTKHKVLE EKGQAVFRNR
     LSKLKWTEEK PSSKNVLEAL KKVHVLCGKK ASLGSTGSSI SQLLLKLLAD TPYLKEGVEV
     YLKSFLLWIQ EPSKSHYNAN IFHDFINWGA QQRLKHQQTS TAASSPQKTG HHENEKTNH