ID   DPOA2_CAEEL             Reviewed;         581 AA.
AC   Q21625;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   06-JUN-2002, sequence version 3.
DT   03-AUG-2022, entry version 137.
DE   RecName: Full=DNA polymerase alpha subunit B;
DE   AltName: Full=Division delayed protein 1;
GN   Name=div-1; ORFNames=R01H10.1/R01H10.2/Y70G10A.4;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=11112326; DOI=10.1006/dbio.2000.9965;
RA   Encalada S.E., Martin P.R., Phillips J.B., Lyczak R., Hamill D.R.,
RA   Swan K.A., Bowerman B.;
RT   "DNA replication defects delay cell division and disrupt cell polarity in
RT   early Caenorhabditis elegans embryos.";
RL   Dev. Biol. 228:225-238(2000).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=12747829; DOI=10.1016/s0960-9822(03)00295-1;
RA   Brauchle M., Baumer K., Goenczy P.;
RT   "Differential activation of the DNA replication checkpoint contributes to
RT   asynchrony of cell division in C. elegans embryos.";
RL   Curr. Biol. 13:819-827(2003).
CC   -!- FUNCTION: May play an essential role at the early stage of chromosomal
CC       DNA replication by coupling the polymerase alpha/primase complex to the
CC       cellular replication machinery. Required for the distribution of pie-1
CC       in cell divsion. {ECO:0000269|PubMed:11112326,
CC       ECO:0000269|PubMed:12747829}.
CC   -!- SUBUNIT: DNA polymerase alpha:primase is a four subunit enzyme complex,
CC       which is assembled throughout the cell cycle, and consists of the two
CC       DNA polymerase subunits A and B, and the DNA primase large and small
CC       subunits. Subunit B binds to subunit A (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- DISRUPTION PHENOTYPE: Delayed cell divisions/cytokinesis. Dysfunctional
CC       development of intestinal and pharyngeal cells. Mislocalization of p-
CC       granules to the nuclei at the 4- and 12-cell embryonic growth stages.
CC       Loss of cell polarity during cell divisions.
CC       {ECO:0000269|PubMed:11112326, ECO:0000269|PubMed:12747829}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase alpha subunit B family.
CC       {ECO:0000305}.
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DR   EMBL; Z31590; CAA83467.2; -; Genomic_DNA.
DR   EMBL; AL032660; CAA83467.2; JOINED; Genomic_DNA.
DR   PIR; T27359; T27359.
DR   RefSeq; NP_499268.1; NM_066867.4.
DR   AlphaFoldDB; Q21625; -.
DR   SMR; Q21625; -.
DR   BioGRID; 41633; 3.
DR   IntAct; Q21625; 1.
DR   STRING; 6239.R01H10.1; -.
DR   EPD; Q21625; -.
DR   PaxDb; Q21625; -.
DR   PeptideAtlas; Q21625; -.
DR   EnsemblMetazoa; R01H10.1.1; R01H10.1.1; WBGene00001002.
DR   GeneID; 176439; -.
DR   KEGG; cel:CELE_R01H10.1; -.
DR   UCSC; R01H10.1; c. elegans.
DR   CTD; 176439; -.
DR   WormBase; R01H10.1; CE28840; WBGene00001002; div-1.
DR   eggNOG; KOG1625; Eukaryota.
DR   GeneTree; ENSGT00390000016784; -.
DR   HOGENOM; CLU_014923_3_1_1; -.
DR   InParanoid; Q21625; -.
DR   OMA; KGPTGNY; -.
DR   OrthoDB; 513979at2759; -.
DR   PhylomeDB; Q21625; -.
DR   Reactome; R-CEL-113501; Inhibition of replication initiation of damaged DNA by RB1/E2F1.
DR   Reactome; R-CEL-68952; DNA replication initiation.
DR   Reactome; R-CEL-68962; Activation of the pre-replicative complex.
DR   Reactome; R-CEL-69091; Polymerase switching.
DR   Reactome; R-CEL-69166; Removal of the Flap Intermediate.
DR   Reactome; R-CEL-69183; Processive synthesis on the lagging strand.
DR   PRO; PR:Q21625; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00001002; Expressed in embryo and 4 other tissues.
DR   GO; GO:0005658; C:alpha DNA polymerase:primase complex; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR   Gene3D; 1.10.8.530; -; 1.
DR   InterPro; IPR007185; DNA_pol_a/d/e_bsu.
DR   InterPro; IPR043034; DNA_pol_alpha_B_N_sf.
DR   InterPro; IPR016722; DNA_pol_alpha_bsu.
DR   PANTHER; PTHR23061; PTHR23061; 1.
DR   Pfam; PF04042; DNA_pol_E_B; 1.
DR   PIRSF; PIRSF018300; DNA_pol_alph_2; 1.
PE   3: Inferred from homology;
KW   DNA replication; Nucleus; Reference proteome.
FT   CHAIN           1..581
FT                   /note="DNA polymerase alpha subunit B"
FT                   /id="PRO_0000194038"
SQ   SEQUENCE   581 AA;  64103 MW;  1D4C581B9C977CD0 CRC64;
     MAYEFDLEDF NDNLEPFGFN CPAKDHFIEK LEGLCKTFRK SGEDIVDDVV SVMSNLGQKS
     VDSGILAKLE ENLEAQAQKT VHTPRSVKKP ARGRVPLAEK SGFILSGDPD DIEKPSHHLT
     NSAIAKLEGH FLDFSPFPGS PANEKFLKRA DPSHVVTTIR GKKYQDKGLK GSTKSDDTIR
     IISKTPSTLY GGDKSSMVIE AKAQRMADIA HRIQKSFEEI VDWGNPSIPS VDVVYTYGQV
     IHDETKDNEK FGEHSVALMI NDEDGTMIRM DFSKMTEDIT LFPGQIIAVR GTNETGEELQ
     VDKIFQPAAL PVNPVETDTT KEIWFACGPY TATDNCGYEH LCELLDKVVA EKPDILMLAG
     PFVDKKNTFL NKPTFNITYD NLLEDLLLKV KETLVGTKTQ VIIQPNASRD LCVPPVFPSA
     PFQQNRKLDK IKKELIFVAD PCIFRISGVE VAMTSSEPIQ ALSNTEFHRS ANQENIDRVA
     RLSSHLLTQQ CMYPLEPTEV PASMGDLLDV CCIGSTPHIV FAPTKLAPSA KCVNGSVFIN
     SSTLAKGPTG NYAKMSINLN AGELMPGETV ADYAQIQILK I