DPOD2_DROME
ID DPOD2_DROME Reviewed; 431 AA.
AC Q9W088; Q8MZ80; Q9U9Q3; T2GGB6;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=DNA polymerase delta subunit 2 {ECO:0000312|FlyBase:FBgn0027903};
DE AltName: Full=Pol31 polymerase delta subunit {ECO:0000303|PubMed:31100062};
GN Name=PolD2 {ECO:0000312|FlyBase:FBgn0027903};
GN Synonyms=DNApolD1 {ECO:0000305}, Pol31 {ECO:0000303|PubMed:31100062};
GN ORFNames=CG12018 {ECO:0000312|FlyBase:FBgn0027903};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head, and Testis;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (SEP-2013) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, IDENTIFICATION IN THE DNA POLYMERASE DELTA COMPLEX, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=31100062; DOI=10.1371/journal.pgen.1008169;
RA Ji J., Tang X., Hu W., Maggert K.A., Rong Y.S.;
RT "The processivity factor Pol32 mediates nuclear localization of DNA
RT polymerase delta and prevents chromosomal fragile site formation in
RT Drosophila development.";
RL PLoS Genet. 15:E1008169-E1008169(2019).
CC -!- FUNCTION: Accessory component of both the DNA polymerase delta complex
CC and possibly the DNA polymerase zeta complex (By similarity). As a
CC component of the delta complex, participates in high fidelity genome
CC replication, including lagging strand synthesis, DNA recombination and
CC repair (By similarity). Appears to promote the function of the DNA pol-
CC delta complex accessory subunit PolD3 in both embryonic and
CC postembryonic somatic cells (PubMed:31100062).
CC {ECO:0000250|UniProtKB:P49005, ECO:0000269|PubMed:31100062}.
CC -!- SUBUNIT: Component of both the DNA polymerase delta and DNA polymerase
CC zeta complexes (By similarity). The DNA polymerase delta complex
CC consisting of three subunits: the catalytic subunit PolD1 and two
CC accessory subunits PolD2/Pol31 and PolD3/Pol32 (PubMed:31100062).
CC Within the delta complex, interacts with both PolD1 and PolD3, and is
CC able to interact with PolD1 in the absence of PolD3 (PubMed:31100062).
CC Component of the DNA polymerase zeta complex consisting of four
CC subunits: the catalytic subunit PolZ1 and three accessory subunits
CC PolZ2/Rev7, PolD2/Pol31 and PolD3/Pol32 (By similarity).
CC {ECO:0000250|UniProtKB:P49005, ECO:0000269|PubMed:31100062}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:31100062}. Nucleus,
CC nucleoplasm {ECO:0000269|PubMed:31100062}. Note=In embryos, accumulates
CC in the nucleus during interphase but disperses into the nucleoplasm at
CC the onset of mitosis. {ECO:0000269|PubMed:31100062}.
CC -!- TISSUE SPECIFICITY: Expressed in ovaries and embryos (at the protein
CC level). {ECO:0000269|PubMed:31100062}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown in postembryonic cells
CC reduces the protein levels of the delta complex member PolD3.
CC {ECO:0000269|PubMed:31100062}.
CC -!- SIMILARITY: Belongs to the DNA polymerase delta/II small subunit
CC family. {ECO:0000305}.
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DR EMBL; AE014296; AAF47566.1; -; Genomic_DNA.
DR EMBL; AF160903; AAD46843.2; -; mRNA.
DR EMBL; AY113311; AAM29316.1; -; mRNA.
DR EMBL; BT150296; AGW24078.1; -; mRNA.
DR RefSeq; NP_647659.1; NM_139402.3.
DR AlphaFoldDB; Q9W088; -.
DR SMR; Q9W088; -.
DR BioGRID; 63760; 5.
DR DIP; DIP-20333N; -.
DR IntAct; Q9W088; 1.
DR STRING; 7227.FBpp0072658; -.
DR PaxDb; Q9W088; -.
DR PRIDE; Q9W088; -.
DR EnsemblMetazoa; FBtr0072775; FBpp0072658; FBgn0027903.
DR GeneID; 38228; -.
DR KEGG; dme:Dmel_CG12018; -.
DR UCSC; CG12018-RA; d. melanogaster.
DR CTD; 38228; -.
DR FlyBase; FBgn0027903; PolD2.
DR VEuPathDB; VectorBase:FBgn0027903; -.
DR eggNOG; KOG2732; Eukaryota.
DR GeneTree; ENSGT00390000006780; -.
DR HOGENOM; CLU_021763_0_0_1; -.
DR InParanoid; Q9W088; -.
DR OMA; DPANFML; -.
DR OrthoDB; 736068at2759; -.
DR PhylomeDB; Q9W088; -.
DR Reactome; R-DME-69166; Removal of the Flap Intermediate.
DR Reactome; R-DME-69183; Processive synthesis on the lagging strand.
DR BioGRID-ORCS; 38228; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38228; -.
DR PRO; PR:Q9W088; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0027903; Expressed in secondary oocyte and 22 other tissues.
DR Genevisible; Q9W088; DM.
DR GO; GO:0043625; C:delta DNA polymerase complex; IDA:FlyBase.
DR GO; GO:0042575; C:DNA polymerase complex; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0016035; C:zeta DNA polymerase complex; ISS:FlyBase.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0006271; P:DNA strand elongation involved in DNA replication; IBA:GO_Central.
DR GO; GO:1990506; P:mitotic DNA-templated DNA replication; IMP:FlyBase.
DR CDD; cd07387; MPP_PolD2_C; 1.
DR InterPro; IPR007185; DNA_pol_a/d/e_bsu.
DR InterPro; IPR040663; DNA_pol_D_N.
DR InterPro; IPR024826; DNA_pol_delta/II_ssu.
DR InterPro; IPR041863; PolD2_C.
DR PANTHER; PTHR10416; PTHR10416; 1.
DR Pfam; PF18018; DNA_pol_D_N; 1.
DR Pfam; PF04042; DNA_pol_E_B; 1.
PE 1: Evidence at protein level;
KW DNA replication; Nucleus; Reference proteome.
FT CHAIN 1..431
FT /note="DNA polymerase delta subunit 2"
FT /id="PRO_0000096170"
FT CONFLICT 363
FT /note="E -> D (in Ref. 3; AAM29316)"
FT /evidence="ECO:0000305"
FT CONFLICT 393
FT /note="E -> D (in Ref. 3; AAM29316)"
FT /evidence="ECO:0000305"
FT CONFLICT 423
FT /note="M -> I (in Ref. 3; AAM29316)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 431 AA; 48009 MW; D0A6EC18EF1EFAFE CRC64;
MTNRLETKYE NLSSIFRLKG HDYQKQFFHL YAHRLAEMTR LLTPLAQKKW GNKEPIKKLC
ELRGEQDVHC ILIGTIFKHQ AHKPSILRDI SEENQLAPQP PRQNYSDPED KIVLEDELQR
VRLQGKLNGQ LLATGVVCAA LGGTDSDGFF NVEDILFYES GPQKPLAPIK RSRLLVLASG
LDQLQAHKFV EALNLFQYWL AGSLGNNKEA GSMVRLIVAG NSVRASAMAH VPTLQVARTQ
ANANDTVQAV SQLDQWFASW ARSLPVDIMP GPYDPANFML PQQPFHKCMF PQAAQLATFQ
AVTNPYSCRL DEALVVGTSG QNVSDLLRST SLDSALEALR CTLTWGHVAP TAPDTLACYP
YIESDPFILR ECPHVYYAGN CESFATELHE GSEGKRTRLV CVPSFSKTQS VAVVDLDTLD
CRMVNFSVDA E
