ID   DPOE2_MOUSE             Reviewed;         527 AA.
AC   O54956; Q6P3Y7; Q8BP72;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2005, sequence version 2.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=DNA polymerase epsilon subunit 2;
DE   AltName: Full=DNA polymerase II subunit 2;
DE   AltName: Full=DNA polymerase epsilon subunit B;
GN   Name=Pole2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Jaw;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 2-527.
RX   PubMed=9443964; DOI=10.1093/nar/26.3.730;
RA   Jokela M., Makiniemi M., Lehtonen S., Szpirer C., Hellman U.,
RA   Syvaeoja J.E.;
RT   "The small subunits of human and mouse DNA polymerase epsilon are
RT   homologous to the second largest subunit of the yeast Saccharomyces
RT   cerevisiae DNA polymerase epsilon.";
RL   Nucleic Acids Res. 26:730-734(1998).
CC   -!- FUNCTION: Accessory component of the DNA polymerase epsilon complex (By
CC       similarity). Participates in DNA repair and in chromosomal DNA
CC       replication (By similarity). {ECO:0000250|UniProtKB:P24482,
CC       ECO:0000250|UniProtKB:P56282}.
CC   -!- SUBUNIT: Component of the DNA polymerase epsilon complex consisting of
CC       four subunits: the catalytic subunit POLE and the accessory subunits
CC       POLE2, POLE3 and POLE4. {ECO:0000250|UniProtKB:P56282}.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- MISCELLANEOUS: In eukaryotes there are five DNA polymerases: alpha,
CC       beta, gamma, delta, and epsilon which are responsible for different
CC       reactions of DNA synthesis.
CC   -!- SIMILARITY: Belongs to the DNA polymerase epsilon subunit B family.
CC       {ECO:0000305}.
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DR   EMBL; AK077592; BAC36883.1; -; mRNA.
DR   EMBL; BC063772; AAH63772.1; -; mRNA.
DR   EMBL; AF036898; AAC40045.1; -; mRNA.
DR   CCDS; CCDS36463.1; -.
DR   RefSeq; NP_035263.1; NM_011133.2.
DR   AlphaFoldDB; O54956; -.
DR   SMR; O54956; -.
DR   BioGRID; 202293; 5.
DR   ComplexPortal; CPX-2109; DNA polymerase epsilon complex.
DR   CORUM; O54956; -.
DR   STRING; 10090.ENSMUSP00000021359; -.
DR   PhosphoSitePlus; O54956; -.
DR   EPD; O54956; -.
DR   MaxQB; O54956; -.
DR   PaxDb; O54956; -.
DR   PeptideAtlas; O54956; -.
DR   PRIDE; O54956; -.
DR   ProteomicsDB; 279568; -.
DR   Antibodypedia; 23530; 134 antibodies from 23 providers.
DR   DNASU; 18974; -.
DR   Ensembl; ENSMUST00000021359; ENSMUSP00000021359; ENSMUSG00000020974.
DR   GeneID; 18974; -.
DR   KEGG; mmu:18974; -.
DR   UCSC; uc007nrv.1; mouse.
DR   CTD; 5427; -.
DR   MGI; MGI:1197514; Pole2.
DR   VEuPathDB; HostDB:ENSMUSG00000020974; -.
DR   eggNOG; KOG3818; Eukaryota.
DR   GeneTree; ENSGT00390000012435; -.
DR   HOGENOM; CLU_010628_2_0_1; -.
DR   InParanoid; O54956; -.
DR   OMA; CRLQYCT; -.
DR   OrthoDB; 375960at2759; -.
DR   PhylomeDB; O54956; -.
DR   TreeFam; TF103007; -.
DR   Reactome; R-MMU-110314; Recognition of DNA damage by PCNA-containing replication complex.
DR   Reactome; R-MMU-5651801; PCNA-Dependent Long Patch Base Excision Repair.
DR   Reactome; R-MMU-5656169; Termination of translesion DNA synthesis.
DR   Reactome; R-MMU-5685942; HDR through Homologous Recombination (HRR).
DR   Reactome; R-MMU-5696397; Gap-filling DNA repair synthesis and ligation in GG-NER.
DR   Reactome; R-MMU-5696400; Dual Incision in GG-NER.
DR   Reactome; R-MMU-6782135; Dual incision in TC-NER.
DR   Reactome; R-MMU-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR   Reactome; R-MMU-68952; DNA replication initiation.
DR   Reactome; R-MMU-68962; Activation of the pre-replicative complex.
DR   BioGRID-ORCS; 18974; 55 hits in 108 CRISPR screens.
DR   ChiTaRS; Pole2; mouse.
DR   PRO; PR:O54956; -.
DR   Proteomes; UP000000589; Chromosome 12.
DR   RNAct; O54956; protein.
DR   Bgee; ENSMUSG00000020974; Expressed in mesenchyme of shoulder and 109 other tissues.
DR   ExpressionAtlas; O54956; baseline and differential.
DR   Genevisible; O54956; MM.
DR   GO; GO:0008622; C:epsilon DNA polymerase complex; ISS:UniProtKB.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR   GO; GO:0016604; C:nuclear body; ISO:MGI.
DR   GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006261; P:DNA-templated DNA replication; ISO:MGI.
DR   GO; GO:0042276; P:error-prone translesion synthesis; IBA:GO_Central.
DR   InterPro; IPR007185; DNA_pol_a/d/e_bsu.
DR   InterPro; IPR024639; DNA_pol_e_bsu_N.
DR   InterPro; IPR016266; POLE2.
DR   PANTHER; PTHR12708; PTHR12708; 1.
DR   Pfam; PF04042; DNA_pol_E_B; 1.
DR   Pfam; PF12213; Dpoe2NT; 1.
DR   PIRSF; PIRSF000799; DNA_pol_eps_2; 1.
PE   2: Evidence at transcript level;
KW   DNA replication; DNA-binding; Nucleus; Reference proteome.
FT   CHAIN           1..527
FT                   /note="DNA polymerase epsilon subunit 2"
FT                   /id="PRO_0000071563"
FT   CONFLICT        78
FT                   /note="M -> V (in Ref. 2; AAH63772)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   527 AA;  59387 MW;  82940B0FD1584350 CRC64;
     MAPERLRSRT VSAFKLRGLL LRSEATKYLT EALQSVTELE LEENLGKIID AVEKQPLSSN
     MIERSVVEAA VQECSQSMDE TIEHVFNIIG AFDIPRFIYN SERKKFLPLS MTNHAAPTLF
     GTARDKAELY LERYTILHQR THRHELFTPP VIGSHLEESG SKFQLKTIET LLGSTSKVGD
     VIVLGMITQL KEGKFFLEDP TGTVQLDLSK AQFHSGLYTE ACFVLAEGWF EDQVFHANAF
     GFPPTEPSST TRAYYGNINF FGGPSNASVK TYTKLRQLED ENKDAMFVIV SDVWLDQVQV
     LEKFHVMFSG YSPAPPTCFI LCGNFSSAPY GKNQVQALKD SLKSLADIIC EYPSIHQSSR
     FVFVPGPEDP GFGSILPRPP LAESITQEFR QRVPFSVFTT NPCRIQYCTQ EIIIFREDLV
     NKMCRNCVRF PSSSLDIPTH FVKTILSQGH LAPLPLYVCP VHWAYDYTLR VYPVPDLLVI
     ADKYDPFTVT NTDCLCINPG SFPRSGFAFK VFYPSSKTVE DSKLQGF