DPOE4_DROME
ID DPOE4_DROME Reviewed; 155 AA.
AC Q9W256; Q8MSF2;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 200.
DE RecName: Full=DNA polymerase epsilon subunit 4 {ECO:0000312|FlyBase:FBgn0034726};
DE AltName: Full=DNA polymerase II subunit 4 {ECO:0000250|UniProtKB:Q9NR33};
DE AltName: Full=Mesoderm-expressed 4 {ECO:0000303|PubMed:19150446};
GN Name=PolE4 {ECO:0000312|FlyBase:FBgn0034726};
GN Synonyms=DNApolE4 {ECO:0000305}, Mes4 {ECO:0000303|PubMed:19150446},
GN NF-Yc {ECO:0000312|FlyBase:FBgn0034726};
GN ORFNames=CG11301 {ECO:0000312|FlyBase:FBgn0034726};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAY55161.1, ECO:0000312|EMBL:ANY27717.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Booth B., Calderwood M., Carlson J., Celniker S., Frise E., George R.,
RA Hao T., Hu Y., Hill D., Mohr S., Pacleb J., Park S., Perrimon N.,
RA Stapleton M., Spirohn K., Yu C., Wan K., Vidal M.;
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000312|EMBL:AAM50718.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 6-155.
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM50718.1};
RC TISSUE=Ovary {ECO:0000312|EMBL:AAM50718.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=19150446; DOI=10.1016/j.yexcr.2008.12.017;
RA Suyari O., Ida H., Yoshioka Y., Kato Y., Hashimoto R., Yamaguchi M.;
RT "Identification of the Drosophila Mes4 gene as a novel target of the
RT transcription factor DREF.";
RL Exp. Cell Res. 315:1403-1414(2009).
CC -!- FUNCTION: Accessory component of the DNA polymerase epsilon complex (By
CC similarity). Participates in DNA repair and in chromosomal DNA
CC replication (By similarity). Has a role in cell cycle progression
CC (PubMed:19150446). Required for wing morphogenesis (PubMed:19150446).
CC {ECO:0000250|UniProtKB:P27344, ECO:0000250|UniProtKB:Q9NRF9,
CC ECO:0000269|PubMed:19150446}.
CC -!- SUBUNIT: Component of the DNA polymerase epsilon complex consisting of
CC four subunits: the catalytic subunit PolE1/DNApol-epsilon255 and the
CC accessory subunits PolE2/DNApol-epsilon58, Chrac-14/DNApolE3 and
CC PolE4/Mes4. {ECO:0000250|UniProtKB:Q9NR33}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19150446}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout all developmental stages
CC (PubMed:19150446). In the third instar larva expressed in fat bodies,
CC brain lobes, eye-antenna imaginal disks, wing imaginal disks, salivary
CC glands and ovaries (PubMed:19150446). {ECO:0000269|PubMed:19150446}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in an atrophied
CC wing phenotype characterized by increased induction of cell
CC proliferation in wing disks but failed cell cycle progression.
CC {ECO:0000269|PubMed:19150446}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE013599; AAF46843.1; -; Genomic_DNA.
DR EMBL; AE013599; ACZ94525.1; -; Genomic_DNA.
DR EMBL; AY118858; AAM50718.1; -; mRNA.
DR EMBL; BT022745; AAY55161.1; -; mRNA.
DR EMBL; KX531907; ANY27717.1; -; mRNA.
DR RefSeq; NP_001163253.1; NM_001169782.2.
DR RefSeq; NP_611669.1; NM_137825.4.
DR AlphaFoldDB; Q9W256; -.
DR SMR; Q9W256; -.
DR IntAct; Q9W256; 22.
DR STRING; 7227.FBpp0290461; -.
DR PaxDb; Q9W256; -.
DR DNASU; 37560; -.
DR EnsemblMetazoa; FBtr0071827; FBpp0071738; FBgn0034726.
DR EnsemblMetazoa; FBtr0301243; FBpp0290461; FBgn0034726.
DR GeneID; 37560; -.
DR KEGG; dme:Dmel_CG11301; -.
DR UCSC; CG11301-RA; d. melanogaster.
DR CTD; 37560; -.
DR FlyBase; FBgn0034726; PolE4.
DR VEuPathDB; VectorBase:FBgn0034726; -.
DR eggNOG; KOG1658; Eukaryota.
DR GeneTree; ENSGT00940000160888; -.
DR HOGENOM; CLU_045277_8_1_1; -.
DR InParanoid; Q9W256; -.
DR OMA; MARIRNI; -.
DR OrthoDB; 1622159at2759; -.
DR PhylomeDB; Q9W256; -.
DR Reactome; R-DME-110314; Recognition of DNA damage by PCNA-containing replication complex.
DR Reactome; R-DME-5651801; PCNA-Dependent Long Patch Base Excision Repair.
DR Reactome; R-DME-5656169; Termination of translesion DNA synthesis.
DR Reactome; R-DME-5696400; Dual Incision in GG-NER.
DR Reactome; R-DME-6782135; Dual incision in TC-NER.
DR Reactome; R-DME-68952; DNA replication initiation.
DR Reactome; R-DME-68962; Activation of the pre-replicative complex.
DR BioGRID-ORCS; 37560; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Mes4; fly.
DR GenomeRNAi; 37560; -.
DR PRO; PR:Q9W256; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034726; Expressed in secondary oocyte and 38 other tissues.
DR GO; GO:0008622; C:epsilon DNA polymerase complex; ISS:FlyBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0006261; P:DNA-templated DNA replication; ISS:FlyBase.
DR Gene3D; 1.10.20.10; -; 1.
DR InterPro; IPR003958; CBFA_NFYB_domain.
DR InterPro; IPR009072; Histone-fold.
DR Pfam; PF00808; CBFD_NFYB_HMF; 1.
DR SUPFAM; SSF47113; SSF47113; 1.
PE 2: Evidence at transcript level;
KW Nucleus; Reference proteome.
FT CHAIN 1..155
FT /note="DNA polymerase epsilon subunit 4"
FT /evidence="ECO:0000305"
FT /id="PRO_0000448436"
FT REGION 1..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 27..48
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 49..64
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 57
FT /note="T -> A (in Ref. 4; AAM50718)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 155 AA; 17370 MW; A41BC0BD24167493 CRC64;
MASEELFEAE FSEEQDLEHQ QAMETEEAEL AETEEPLEIT EESPDNPEAE STTEQLTEKP
VTNGNKAPAD NEAKMTQLPL ARIRNIMKLD PDLHMANNEA VFIVAKAVEL FIASLSRESY
TYTAQSKKKT IQKRDVDMAI SAVDSLLFLD GAMNF
