DPOE_CRYNB
ID DPOE_CRYNB Reviewed; 2250 AA.
AC P0CN27; Q55JX3; Q5K9M7;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 59.
DE RecName: Full=DNA polymerase epsilon catalytic subunit A;
DE EC=2.7.7.7 {ECO:0000250|UniProtKB:P15436};
DE AltName: Full=DNA polymerase II subunit A;
GN Name=POL2; OrderedLocusNames=CNBK2100;
OS Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
OS (Filobasidiella neoformans).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=283643;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B-3501A;
RX PubMed=15653466; DOI=10.1126/science.1103773;
RA Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT neoformans.";
RL Science 307:1321-1324(2005).
CC -!- FUNCTION: DNA polymerase II participates in chromosomal DNA
CC replication. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000250|UniProtKB:P15436};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000250|UniProtKB:P15436};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250|UniProtKB:P15436};
CC -!- SUBUNIT: Heterotetramer. Consists of 4 subunits: POL2, DPB2, DPB3 and
CC DPB4 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DOMAIN: The CysA-type zinc finger is required for PCNA-binding.
CC {ECO:0000250|UniProtKB:P15436}.
CC -!- DOMAIN: The CysB motif binds 1 4Fe-4S cluster and is required for the
CC formation of polymerase complexes. {ECO:0000250|UniProtKB:P15436}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
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DR EMBL; AAEY01000052; EAL18192.1; -; Genomic_DNA.
DR RefSeq; XP_772839.1; XM_767746.1.
DR AlphaFoldDB; P0CN27; -.
DR SMR; P0CN27; -.
DR EnsemblFungi; AAW46304; AAW46304; CNK01430.
DR EnsemblFungi; EAL18192; EAL18192; CNBK2100.
DR GeneID; 4938907; -.
DR KEGG; cnb:CNBK2100; -.
DR VEuPathDB; FungiDB:CNBK2100; -.
DR HOGENOM; CLU_000556_0_1_1; -.
DR Proteomes; UP000001435; Chromosome 11.
DR GO; GO:0008622; C:epsilon DNA polymerase complex; IEA:InterPro.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR Gene3D; 1.10.132.60; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR Gene3D; 3.90.1600.10; -; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR042087; DNA_pol_B_thumb.
DR InterPro; IPR013697; DNA_pol_e_suA_C.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR029703; POL2.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR10670; PTHR10670; 1.
DR Pfam; PF00136; DNA_pol_B; 1.
DR Pfam; PF03104; DNA_pol_B_exo1; 1.
DR Pfam; PF08490; DUF1744; 1.
DR SMART; SM01159; DUF1744; 1.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; DNA replication; DNA-binding; DNA-directed DNA polymerase; Iron;
KW Iron-sulfur; Metal-binding; Nucleotidyltransferase; Nucleus; Transferase;
KW Zinc; Zinc-finger.
FT CHAIN 1..2250
FT /note="DNA polymerase epsilon catalytic subunit A"
FT /id="PRO_0000410067"
FT ZN_FING 2118..2159
FT /note="CysA-type"
FT /evidence="ECO:0000250|UniProtKB:P15436"
FT REGION 1..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 235..259
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1990..2010
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 2190..2207
FT /note="CysB motif"
FT /evidence="ECO:0000250|UniProtKB:P15436"
FT COMPBIAS 236..259
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 2118
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P15436"
FT BINDING 2121
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P15436"
FT BINDING 2156
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P15436"
FT BINDING 2159
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P15436"
FT BINDING 2190
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250|UniProtKB:P15436"
FT BINDING 2193
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250|UniProtKB:P15436"
FT BINDING 2205
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250|UniProtKB:P15436"
FT BINDING 2207
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250|UniProtKB:P15436"
SQ SEQUENCE 2250 AA; 255999 MW; 30EED4407247B80B CRC64;
MPSRGSFRGR GRGSGSGFGS NTRFTGRRRG RGGGGGGGGV AYGIDRRPAT STNQANREDG
TAATEKFEEV KVYDEIDEKI GFWRFESVRA EGEEKVGWLV NMHQTLVPSD VHPGGLAAVD
YYFIQDDGGS FKVSIPYEPY FYLTCRGGTE SIVEEWLLKR YEGIIIRIER EKKWDLSLPN
HLLSAPPVFL KLFFHNTADL QTIRRDLLPL ARSNSEKFTA VDAYADVVSA EAAANGHGDD
ENRAWGAEDD TKKKKDKEPS ECIIEVREHD LAYHLRVAID LNIRVGLWYT VTSRTGVITL
ERIPDRVKRA DPVVMAYDIE TTKQPLKFPD QQTDQIMMIS YMIDGMGYLI TNREIVGEDI
DDFEYTPKDE YPGEFTVFNE PDEPAVIRRW FEHIRDSKPT VIATYNGDSF DFPFVDARAK
IHGISMYEEI GFKPDIEEEY KSRSTMHMDC FRWVKRDSYL PQGSQGLKAV TTAKLGYNPI
ELDPELMTPY AIEQPQILAQ YSVSDAVATY YLYMKYVHPF IFSLCNIIPL SPDEVLRKGT
GTLCETLLMV EAYDAHIIMP NRHEDPHGVT YEGHLLASET YVGGHVEALE AGVFRSDIPT
HFKIVPSAIQ ELIDDLDAAL RFSLIEEGQV KLEDVENYDE VKQQIQTALE TMRDEPNRFD
NPLIYHLDVA AMYPNIMLSN RLQPDSVKEE ADCAVCDYNR PDKKCDRRME WAWRGEYFPA
KRDEVNMVRY ALEQETFPPK RPYDPKRRFV DLTPTEQSAL LHKRLGDYSR KVYKKTHDTK
IVTKTTIICQ RENSFYIDTV RAFRDRRYEY KGLHKTWKKN LDKAFEEGGA VATVDEAKKM
IVLYDSLQLA HKCILNSFYG YVMRKGARWY SMEMAGITCL TGATIIQMAR QLVEQIGRPL
ELDTDGIWCM LPGVFPEDFN FKLKNGKKFG ISYPCTMLNH LVHAQFTNDQ YHELVNNDSG
TYNVKKENSI FFELDGPYKA MILPSSKEED KLLKKRYAVF NPDGSLAELK GFEVKRRGEL
QMIKIFQSQI FDKFLLGKTT EECYAAVATV ADQWLDILQS KASSLHDDEL VDLIAENRSM
SKTLAEYAGQ KSTSISTARR LAEFLGEQMV KDKGLSCRFI ISAKPNGAPV TERAVPVAIF
TAEEPVKRHY LRKWLKDNSL TDFDLRTILD WDYYTERLGS VIQKLITIPA ALQKVPNPVP
RIRHPDWLYK RIATKEDKFQ QHKITDMFTK LKDMEDLGDG QKKVGPKLAV VRRRNRKEQE
KESEVEEVPP EPEYDYAGYI RIMKKKWRKQ RQEKARARKS GLRQDGTISS MLRTQTSSMN
SKQWDVIQIA STNRPGEFKL WLAIDGTFQS VRLKVPREFY LNFKEDPEPQ MLATDRYEAV
EVVRTLPRGQ PARHLYKLSV DEVLFMEGES HFSTLINNPN VDGAFELQVP LVVRALLSLG
TSCALRSNIL GGLNRGLDKG FDLVDLERSG NLSRRKYLNE GRGIRYHFLF HAVADQRHII
GLFSPDSTNA SIYLVDRAKN RQQLPNPLKF YTDRVERAER GVFSYPDMLD FTTSYHSSES
SAFKALGKDL QAINHGLNVI ALCSPFEHSY YQAKAPVYSN FPFITYRLGK EEDPGLMWLL
QTSRRMIGLY LRLSSWLKEQ IQIASHFDVP IGNLGADIAV FLADIEFARR LKQQDMLIWW
SSTPRPDLGG SEEDANSSED LVSPHISNRG CYSSTVLEME VSDLAINAVL QSALVNEMEG
SGTGSLAFDS ASHNLDEYAK GAVNTSVMLG DAVLSTQTFG VLKSMLRAWY ADKARAHVKG
DSLSPAEIVV DQFWRWISSS TSSMFEPALH RFLHGLMRKT FLQLLAEFKR LGTQVVYADF
GRVFLLTSKP DAGSAFAFAK YLVAAANSQE LFRHLIIDVA QFWNYLAWMD VANFGGVKVS
PETAASREPP AKRFEISMDW NIQSFLPGTL QPVFERNVAS FIFSLYSAKR SSSDGREPLR
VIHSLNIDQP GTEATSTMNP TKEKEKKAAS KSISQTLTRR LLSDIAAVKR QQAAVHLEPE
EAYTLAFPDL PGARSKRINP TLELIKAITE VYSLASEHSI EVQILKRNLL DLIGVKEFSS
DAAFNPPCES IEVPMVICKK CNAIRDVDLC RDPDRLPSVN PDSGEMLEPA RKNWVCHKCD
SEYDRFQIEQ PLIEMVTKTI TNYQIQDVIC LKCSQTKSDN LAATCKCGGG FRTTSNRNEL
KTKLKMIKSV CDYHKLPFAG SYVEETLSRW
