DPOG1_RAT
ID DPOG1_RAT Reviewed; 1216 AA.
AC Q9QYV8; Q9QYV7;
DT 16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=DNA polymerase subunit gamma-1;
DE EC=2.7.7.7;
DE AltName: Full=Mitochondrial DNA polymerase catalytic subunit;
DE AltName: Full=PolG-alpha;
GN Name=Polg; Synonyms=Mip1, Polg1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT HIS-273.
RC STRAIN=Sprague-Dawley; TISSUE=Liver;
RA Sun Q., Popanda O., Thielmann H.W.;
RT "Mitochondrial DNA polymerase gamma from Novikoff hepatoma cells differs
RT from that of normal rat liver in cDNA sequence and kinetic properties.";
RL Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the replication of mitochondrial DNA. Associates
CC with mitochondrial DNA (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SUBUNIT: Heterotrimer composed of a catalytic subunit and a homodimer
CC of accessory subunits (By similarity). Interacts with TTC3 (By
CC similarity). {ECO:0000250|UniProtKB:P54098,
CC ECO:0000250|UniProtKB:Q27607}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion. Mitochondrion matrix,
CC mitochondrion nucleoid {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ245646; CAB56206.1; -; mRNA.
DR EMBL; AJ245647; CAB56207.1; -; mRNA.
DR RefSeq; NP_445980.1; NM_053528.1.
DR RefSeq; XP_006229472.1; XM_006229410.3.
DR AlphaFoldDB; Q9QYV8; -.
DR SMR; Q9QYV8; -.
DR STRING; 10116.ENSRNOP00000047900; -.
DR PhosphoSitePlus; Q9QYV8; -.
DR PaxDb; Q9QYV8; -.
DR PRIDE; Q9QYV8; -.
DR GeneID; 85472; -.
DR KEGG; rno:85472; -.
DR UCSC; RGD:620057; rat.
DR CTD; 5428; -.
DR RGD; 620057; Polg.
DR VEuPathDB; HostDB:ENSRNOG00000032293; -.
DR eggNOG; KOG3657; Eukaryota.
DR HOGENOM; CLU_001524_2_2_1; -.
DR InParanoid; Q9QYV8; -.
DR OMA; LWLWDED; -.
DR OrthoDB; 86850at2759; -.
DR PhylomeDB; Q9QYV8; -.
DR PRO; PR:Q9QYV8; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Bgee; ENSRNOG00000032293; Expressed in liver and 19 other tissues.
DR Genevisible; Q9QYV8; RN.
DR GO; GO:0005615; C:extracellular space; ISO:RGD.
DR GO; GO:0005760; C:gamma DNA polymerase complex; IDA:RGD.
DR GO; GO:0005759; C:mitochondrial matrix; ISO:RGD.
DR GO; GO:0042645; C:mitochondrial nucleoid; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; IDA:RGD.
DR GO; GO:0005730; C:nucleolus; ISO:RGD.
DR GO; GO:0032991; C:protein-containing complex; ISO:RGD.
DR GO; GO:0043195; C:terminal bouton; IDA:RGD.
DR GO; GO:0008408; F:3'-5' exonuclease activity; ISO:RGD.
DR GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; ISO:RGD.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IDA:RGD.
DR GO; GO:0004527; F:exonuclease activity; ISO:RGD.
DR GO; GO:0002020; F:protease binding; ISO:RGD.
DR GO; GO:0007568; P:aging; IEP:RGD.
DR GO; GO:0006287; P:base-excision repair, gap-filling; ISO:RGD.
DR GO; GO:0071333; P:cellular response to glucose stimulus; IEP:RGD.
DR GO; GO:0006261; P:DNA-templated DNA replication; ISO:RGD.
DR GO; GO:0006264; P:mitochondrial DNA replication; ISO:RGD.
DR GO; GO:0010332; P:response to gamma radiation; IEP:RGD.
DR GO; GO:0055093; P:response to hyperoxia; IEP:RGD.
DR GO; GO:0009416; P:response to light stimulus; IEP:RGD.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR002297; DNA-dir_DNA_pol_A_mt.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR041336; DNApol_Exo.
DR InterPro; IPR012337; RNaseH-like_sf.
DR PANTHER; PTHR10267; PTHR10267; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF18136; DNApol_Exo; 1.
DR PIRSF; PIRSF000797; DNA_pol_mt; 1.
DR PRINTS; PR00867; DNAPOLG.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 2: Evidence at transcript level;
KW DNA replication; DNA-binding; DNA-directed DNA polymerase; Magnesium;
KW Mitochondrion; Mitochondrion nucleoid; Nucleotidyltransferase;
KW Reference proteome; Transferase.
FT CHAIN 1..1216
FT /note="DNA polymerase subunit gamma-1"
FT /id="PRO_0000101272"
FT REGION 27..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 301..321
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 488..518
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 273
FT /note="R -> H (in Novikoff hepatoma cells)"
FT /evidence="ECO:0000269|Ref.1"
SQ SEQUENCE 1216 AA; 136856 MW; A74CD68B6BDB690C CRC64;
MSRLLWKKVA GAKVASGPVP ATGRWVSSSV LDPVPSDGQP QSQMPSSENG QLRLNPLHIQ
MLSRGLHEQI FGCGGDVPDE AAVQRSIEHL RKHGLWGQPT TPLPDVQLRL PRLFGGNLDQ
HFRLLAQKQS LPYLEAAASL SEAQLPPQPR KWVWAEGWTR YGPEGEAEPV AIPEERALVF
DVEVCLAEGT CPTLAVAISP SAWYSWCSRR LVEERYSWTS QLSPADLIPL GVSASASSST
QQDWQEQLVV GHNVSFDRAH IREQYLIQGS RMRFLDTMSM HMAISGLSSF QRSLWMGAKQ
GKHKTQHPTK RGQKSQKNAN GPAISSWDWM DISSANNLAD VHNLYVGGPR LAKEPRELFV
KGSMRDIREN FQDLMEYCAR DVWATFEVFQ QQLPLFLERC PHPVTLAGML EMGVSYLPVN
QNWERYLTEA QSTYEELQRE MKKSLMELAN DACQLLSGER YKEDPWLWDL EWDLQEFKQK
KAKKVKKTAS ASKLPIEGAG PFGDPMDQED PGPPSEEEEL QQNIMAHTRL QQLKSTTDLL
PKRPQHLPGH PGWYRKLCPR LDDPAWTPGP SLLSLQMRVT PKLMALTWDG FPLHYSDSHG
WGYLVPGRRD NLTELPVSPT EESAAVTCPY RAIESLYRRH CLDQGKQQLE TQETDLAEEF
LLTDSAMWQT VEELGCLDVE AEATVESSGL SQPLVPPTAC APKTSQPTYH HGNGPYNDVD
IPGCWFFKLP HKDGNNYNVG SPFAKDFLPK MEDGTLQAGP GGARGPRALE INKMISFWRN
AHKRISSQMV VWLPRSALPR AVTRHPSFDE ESHYGAILPQ VVTAGTITRR AVEPTWLTAS
NARPDRVGSE LKAMVQAPPG YVLVGADVDS QELWIAAVLG DAHFAGMHGC TAFGWMTLQG
RKSRGTDLHS KTAATVGISR EHAKVFNYGR IYGAGQSFAE RLLMQFNHRL SRQEAADKAQ
QMYAVTKGLR RYRLSDDGEW LVKQLNVPVD RTEDGWVSLQ DLRKIRREAS RKSRWKKWEV
VTERAWTGGT ESEMFNKLES IAMSDTPRTP VLGCCISRAL EPSVVQGEFM TSRVNWVVQS
SAVDYLHLML VAMKWLFEEF AIDGRFCISI HDEVRYLVRE EDRYRAALAL QITNLLTRCM
FAYKLGLNDL PQSVAFFSAV DIDQCLRKEV TMDCKTPSNP TGMERKYGIP QGEALDIYQI
IELTKGSLEK RSQPGP
