ID   ADEC_METAC              Reviewed;         553 AA.
AC   Q8TNH5;
DT   16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Adenine deaminase {ECO:0000255|HAMAP-Rule:MF_01518};
DE            Short=Adenase {ECO:0000255|HAMAP-Rule:MF_01518};
DE            Short=Adenine aminase {ECO:0000255|HAMAP-Rule:MF_01518};
DE            EC=3.5.4.2 {ECO:0000255|HAMAP-Rule:MF_01518};
GN   Name=ade {ECO:0000255|HAMAP-Rule:MF_01518}; Synonyms=adeC;
GN   OrderedLocusNames=MA_2310;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenine + H(+) + H2O = hypoxanthine + NH4(+);
CC         Xref=Rhea:RHEA:23688, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16708, ChEBI:CHEBI:17368, ChEBI:CHEBI:28938; EC=3.5.4.2;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01518};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01518};
CC   -!- SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily.
CC       Adenine deaminase family. {ECO:0000255|HAMAP-Rule:MF_01518}.
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DR   EMBL; AE010299; AAM05703.1; -; Genomic_DNA.
DR   RefSeq; WP_011022289.1; NC_003552.1.
DR   AlphaFoldDB; Q8TNH5; -.
DR   SMR; Q8TNH5; -.
DR   STRING; 188937.MA_2310; -.
DR   EnsemblBacteria; AAM05703; AAM05703; MA_2310.
DR   GeneID; 1474199; -.
DR   KEGG; mac:MA_2310; -.
DR   HOGENOM; CLU_027935_0_0_2; -.
DR   InParanoid; Q8TNH5; -.
DR   OMA; TDHECFT; -.
DR   OrthoDB; 12286at2157; -.
DR   PhylomeDB; Q8TNH5; -.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0000034; F:adenine deaminase activity; IBA:GO_Central.
DR   GO; GO:0006146; P:adenine catabolic process; IEA:InterPro.
DR   CDD; cd01295; AdeC; 1.
DR   Gene3D; 2.30.40.10; -; 1.
DR   HAMAP; MF_01518; Adenine_deamin; 1.
DR   InterPro; IPR006679; Adenine_deam.
DR   InterPro; IPR026912; Adenine_deam_C.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   PANTHER; PTHR11113:SF2; PTHR11113:SF2; 1.
DR   Pfam; PF13382; Adenine_deam_C; 1.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   SUPFAM; SSF51338; SSF51338; 1.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   TIGRFAMs; TIGR01178; ade; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Manganese; Reference proteome.
FT   CHAIN           1..553
FT                   /note="Adenine deaminase"
FT                   /id="PRO_0000142439"
SQ   SEQUENCE   553 AA;  60731 MW;  C58B219A5AEF73DB CRC64;
     MKKYLGIIVD AVSRRQFKGE ITVENGKILR VEEKEHDHER YILPGLVDAH VHIESSMTVP
     SVFARMAVAK GTVAVVSDPH EIANVMGEEG IDFMLGDSKR SPLKVFFGVP SCVPATPFES
     AGSVLDAEAV DRLLAREDLY YLSEMMNFPG VVLGFPDVMA KLESAKKCGK VIDGHAPGLR
     GADLETYIGV GISTDHESFT YEEAVEKIKL GMKILIREGS SARNFETLYS LIDEYPEAVM
     LCTDDSHPDT LIYEGHIDKL IRRGQEKGLD IFNLIRAAVI NPVEHYGLNV GLLREGDPAD
     FITVDDLKSF NVLSTFIDGE CVYENGKVLF PMKKVPAKNV FNRNKISIDA AKLAVPAEET
     GDRKEGMRKI RVIVARDGEL VTGQELAFPK VENGNMVSDP EKDILKMVVL SRYADDPVQI
     GFIKNIGLKE GAIASSIAHD SHNIIAVGAT DKDIVEAVNR LVENKGGIVV GTAENLLDLP
     LEVAGLMSTL DGVEVASRYR LLNEEARKLG TSLESPFMTL AFMSLLVIPE LKLGDKGLFD
     VTKFEFVELF AEE