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DPOG_YEAST
ID   DPOG_YEAST              Reviewed;        1254 AA.
AC   P15801; D6W327; Q08785;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 3.
DT   03-AUG-2022, entry version 170.
DE   RecName: Full=DNA polymerase gamma;
DE            EC=2.7.7.7;
DE   AltName: Full=Mitochondrial DNA polymerase catalytic subunit;
GN   Name=MIP1; OrderedLocusNames=YOR330C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=2684980; DOI=10.1016/s0021-9258(19)47098-1;
RA   Foury F.;
RT   "Cloning and sequencing of the nuclear gene MIP1 encoding the catalytic
RT   subunit of the yeast mitochondrial DNA polymerase.";
RL   J. Biol. Chem. 264:20552-20560(1989).
RN   [2]
RP   SEQUENCE REVISION.
RA   Foury F.;
RL   Submitted (JUN-1990) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8896263;
RX   DOI=10.1002/(sici)1097-0061(199609)12:10b<999::aid-yea976>3.0.co;2-e;
RA   Parle-McDermott A.G., Hand N.J., Goulding S.E., Wolfe K.H.;
RT   "Sequence of 29 kb around the PDR10 locus on the right arm of Saccharomyces
RT   cerevisiae chromosome XV: similarity to part of chromosome I.";
RL   Yeast 12:999-1004(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169874;
RA   Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA   Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA   Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA   Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA   Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA   Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA   Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA   Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA   Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA   Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA   Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA   Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA   Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA   Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA   Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA   Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL   Nature 387:98-102(1997).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [6]
RP   NUCLEOTIDE SEQUENCE OF 987-1254.
RC   STRAIN=S288c / GRF88;
RA   Song J.M., Cheung E., Rabinowitz J.C.;
RL   Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: Involved in the replication of mitochondrial DNA.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- MISCELLANEOUS: In eukaryotes there are five DNA polymerases: alpha,
CC       beta, gamma, delta, and epsilon which are responsible for different
CC       reactions of DNA synthesis.
CC   -!- MISCELLANEOUS: Present with 377 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-A family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA99652.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; J05117; AAA17543.1; -; Unassigned_DNA.
DR   EMBL; Z49821; CAA89977.1; -; Genomic_DNA.
DR   EMBL; Z75238; CAA99652.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U42227; AAA85442.1; -; Genomic_DNA.
DR   EMBL; BK006948; DAA11093.1; -; Genomic_DNA.
DR   PIR; S62062; A32686.
DR   RefSeq; NP_014975.2; NM_001183750.1.
DR   AlphaFoldDB; P15801; -.
DR   SMR; P15801; -.
DR   BioGRID; 34715; 159.
DR   IntAct; P15801; 3.
DR   STRING; 4932.YOR330C; -.
DR   iPTMnet; P15801; -.
DR   MaxQB; P15801; -.
DR   PaxDb; P15801; -.
DR   PRIDE; P15801; -.
DR   EnsemblFungi; YOR330C_mRNA; YOR330C; YOR330C.
DR   GeneID; 854508; -.
DR   KEGG; sce:YOR330C; -.
DR   SGD; S000005857; MIP1.
DR   VEuPathDB; FungiDB:YOR330C; -.
DR   eggNOG; KOG3657; Eukaryota.
DR   GeneTree; ENSGT00390000000453; -.
DR   HOGENOM; CLU_001524_2_1_1; -.
DR   InParanoid; P15801; -.
DR   OMA; LWLWDED; -.
DR   BioCyc; YEAST:G3O-33807-MON; -.
DR   PHI-base; PHI:505; -.
DR   PRO; PR:P15801; -.
DR   Proteomes; UP000002311; Chromosome XV.
DR   RNAct; P15801; protein.
DR   GO; GO:0005760; C:gamma DNA polymerase complex; IEA:InterPro.
DR   GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IDA:SGD.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IDA:SGD.
DR   GO; GO:0032043; P:mitochondrial DNA catabolic process; IMP:SGD.
DR   GO; GO:0006264; P:mitochondrial DNA replication; IDA:SGD.
DR   GO; GO:0000002; P:mitochondrial genome maintenance; IMP:SGD.
DR   InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR   InterPro; IPR002297; DNA-dir_DNA_pol_A_mt.
DR   InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR041336; DNApol_Exo.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   PANTHER; PTHR10267; PTHR10267; 1.
DR   Pfam; PF00476; DNA_pol_A; 1.
DR   Pfam; PF18136; DNApol_Exo; 1.
DR   PIRSF; PIRSF000797; DNA_pol_mt; 1.
DR   PRINTS; PR00867; DNAPOLG.
DR   SMART; SM00482; POLAc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE   1: Evidence at protein level;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase; Magnesium;
KW   Mitochondrion; Nucleotidyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..1254
FT                   /note="DNA polymerase gamma"
FT                   /id="PRO_0000101278"
FT   REGION          1125..1145
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1202..1240
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1206..1220
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        8
FT                   /note="S -> F (in Ref. 1; AAA17543)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        35
FT                   /note="T -> A (in Ref. 1; AAA17543)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        222
FT                   /note="I -> V (in Ref. 1; AAA17543)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        357
FT                   /note="E -> K (in Ref. 1; AAA17543)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        540..541
FT                   /note="TH -> MN (in Ref. 1; AAA17543)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        616
FT                   /note="S -> N (in Ref. 1; AAA17543)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        661
FT                   /note="A -> T (in Ref. 1; AAA17543)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        978
FT                   /note="S -> P (in Ref. 1; AAA17543)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        986
FT                   /note="N -> S (in Ref. 1; AAA17543)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1254 AA;  143502 MW;  80EE686E8EA9F2EC CRC64;
     MTKLMVRSEC MLRMVRRRPL RVQFCARWFS TKKNTAEAPR INPVGIQYLG ESLQRQVFGS
     CGGKDEVEQS DKLMELSKKS LKDHGLWGKK TLITDPISFP LPPLQGRSLD EHFQKIGRFN
     SEPYKSFCED KFTEMVARPA EWLRKPGWVK YVPGMAPVEV AYPDEELVVF DVETLYNVSD
     YPTLATALSS TAWYLWCSPF ICGGDDPAAL IPLNTLNKEQ VIIGHNVAYD RARVLEEYNF
     RDSKAFFLDT QSLHIASFGL CSRQRPMFMK NNKKKEAEVE SEVHPEISIE DYDDPWLNVS
     ALNSLKDVAK FHCKIDLDKT DRDFFASTDK STIIENFQKL VNYCATDVTA TSQVFDEIFP
     VFLKKCPHPV SFAGLKSLSK CILPTKLNDW NDYLNSSESL YQQSKVQIES KIVQIIKDIV
     LLKDKPDFYL KDPWLSQLDW TTKPLRLTKK GVPAKCQKLP GFPEWYRQLF PSKDTVEPKI
     TIKSRIIPIL FKLSWENSPV IWSKESGWCF NVPHEQVETY KAKNYVLADS VSQEEEEIRT
     HNLGLQCTGV LFKVPHPNGP TFNCTNLLTK SYNHFFEKGV LKSESELAHQ ALQINSSGSY
     WMSARERIQS QFVVPSCKFP NEFQSLSAKS SLNNEKTNDL AIIIPKIVPM GTITRRAVEN
     AWLTASNAKA NRIGSELKTQ VKAPPGYCFV GADVDSEELW IASLVGDSIF NVHGGTAIGW
     MCLEGTKNEG TDLHTKTAQI LGCSRNEAKI FNYGRIYGAG AKFASQLLKR FNPSLTDEET
     KKIANKLYEN TKGKTKRSKL FKKFWYGGSE SILFNKLESI AEQETPKTPV LGCGITYSLM
     KKNLRANSFL PSRINWAIQS SGVDYLHLLC CSMEYIIKKY NLEARLCISI HDEIRFLVSE
     KDKYRAAMAL QISNIWTRAM FCQQMGINEL PQNCAFFSQV DIDSVIRKEV NMDCITPSNK
     TAIPHGEALD INQLLDKSNS KLGKPNLDID SKVSQYAYNY REPVFEEYNK SYTPEFLKYF
     LAMQVQSDKR DVNRLEDEYL RECTSKEYAR DGNTAEYSLL DYIKDVEKGK RTKVRIMGSN
     FLDGTKNAKA DQRIRLPVNM PDYPTLHKIA NDSAIPEKQL LENRRKKENR IDDENKKKLT
     RKKNTTPMER KYKRVYGGRK AFEAFYECAN KPLDYTLETE KQFFNIPIDG VIDDVLNDKS
     NYKKKPSQAR TASSSPIRKT AKAVHSKKLP ARKSSTTNRN LVELERDITI SREY
 
 
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