ID   DPOL2_AERPE             Reviewed;         803 AA.
AC   O93746;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 3.
DT   25-MAY-2022, entry version 117.
DE   RecName: Full=DNA polymerase 2;
DE            EC=2.7.7.7;
DE   AltName: Full=DNA polymerase II;
GN   Name=polB; OrderedLocusNames=APE_2098.1;
OS   Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 /
OS   K1).
OC   Archaea; Crenarchaeota; Thermoprotei; Desulfurococcales;
OC   Desulfurococcaceae; Aeropyrum.
OX   NCBI_TaxID=272557;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1;
RX   PubMed=10382966; DOI=10.1093/dnares/6.2.83;
RA   Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K.,
RA   Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A.,
RA   Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S.,
RA   Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A.,
RA   Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H.;
RT   "Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon,
RT   Aeropyrum pernix K1.";
RL   DNA Res. 6:83-101(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-803.
RC   STRAIN=ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1;
RA   Ishino Y., Cann I.K.;
RT   "Isolation of the genes encoding two alpha-like DNA polymerases from
RT   Aeropyrum pernix.";
RL   Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
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DR   EMBL; BA000002; BAA81109.2; -; Genomic_DNA.
DR   EMBL; AB017501; BAA75663.1; -; Genomic_DNA.
DR   PIR; E72515; E72515.
DR   AlphaFoldDB; O93746; -.
DR   SMR; O93746; -.
DR   STRING; 272557.APE_2098.1; -.
DR   PRIDE; O93746; -.
DR   EnsemblBacteria; BAA81109; BAA81109; APE_2098.1.
DR   KEGG; ape:APE_2098.1; -.
DR   PATRIC; fig|272557.25.peg.1399; -.
DR   eggNOG; arCOG00328; Archaea.
DR   BRENDA; 2.7.7.7; 171.
DR   Proteomes; UP000002518; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.132.60; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.90.1600.10; -; 1.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR   InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR042087; DNA_pol_B_thumb.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF00136; DNA_pol_B; 1.
DR   Pfam; PF03104; DNA_pol_B_exo1; 1.
DR   PRINTS; PR00106; DNAPOLB.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase;
KW   Nucleotidyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..803
FT                   /note="DNA polymerase 2"
FT                   /id="PRO_0000046475"
FT   CONFLICT        123
FT                   /note="E -> K (in Ref. 2; BAA75663)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        325..338
FT                   /note="KIGERVTLEWWQIG -> R (in Ref. 2; BAA75663)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        374
FT                   /note="E -> Q (in Ref. 2; BAA75663)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        773..778
FT                   /note="LRILQY -> SAHTSSN (in Ref. 2; BAA75663)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        782
FT                   /note="T -> I (in Ref. 2; BAA75663)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   803 AA;  91739 MW;  FB9D8AF49E3681B0 CRC64;
     MKGSDATLLS FQLLDVSYEM RGSTPVIILW GRGADGSRVV VFYGEFRPYF YVLPDGSVGL
     DQLAAMIRRL SRPSSPILSV ERVRRRFIGR EVEALKVTTL VPASVREYRE AVRRLGGVRD
     VLEADIPFAL RFIIDFNLYP MRWYVAEVRE VAVPHGYSVD RAYTLSGDIR EDETRIQEDP
     LKGLRVMAFD IEVYSKMRTP DPKKDPVIMI GLQQAGGEIE ILEAEDRSDK KVIAGFVERV
     KSIDPDVIVG YNQNRFDWPY LVERARVLGV KLAVGRRSVE PQPGLYGHYS VSGRLNVDLL
     DFAEELHEVK VKTLEEVADY LGVVKIGERV TLEWWQIGEY WDDPSKREIL RKYLRDDVRS
     TMGLAEKFLP FGAELSQVSG LPLDQVMAAS VGFRLEWRLI REAAKLGELV PNRVERSEGR
     YAGAIVLRPK PGVHEDIAVL DFASMYPNIM VKYNVGPDTL VRPGEEYGEE EVYTAPEVGH
     KFRKSPPGFF KKILERFLSW RRQIRSEMKK HPPDSPEYKL LDERQKAIKL LANASYGYMG
     WPHARWYCRE CAEAVTAWGR SIIRTAIRKA GELGLEVIYG DTDSLFVKND PEKVERLIRF
     VEEELGFDIK VDKVYRRVFF TEAKKRYVGL TVDGKIDVVG FEAVRGDWSE LAKETQFKVA
     EIVLKTGSVD EAVDYVRNII EKLRRGQVDM RKLVIWKTLT RPPSMYEARQ PHVTAALLME
     RAGIKVEPGA KIGYVVTKGS GPLYTRAKPY FMASKEEVDV EYYVDKQVVP AALRILQYFG
     VTEKRLKGGG RQSTLLDFMR RGK