DPOL2_SACS2
ID DPOL2_SACS2 Reviewed; 626 AA.
AC Q07635;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2001, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=DNA polymerase 2;
DE EC=2.7.7.7;
DE AltName: Full=DNA polymerase B2;
DE AltName: Full=DNA polymerase II;
GN Name=dpo2; OrderedLocusNames=SSO1459/SSO8124;
OS Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS (Sulfolobus solfataricus).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Saccharolobus.
OX NCBI_TaxID=273057;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=8332474; DOI=10.1093/nar/21.11.2768;
RA Prangishvili D., Klenk H.-P.;
RT "Nucleotide sequence of the gene for a 74 kDa DNA polymerase from the
RT archaeon Sulfolobus solfataricus.";
RL Nucleic Acids Res. 21:2768-2768(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=11427726; DOI=10.1073/pnas.141222098;
RA She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
CC -!- FUNCTION: This polymerase is devoid of exonuclease activity.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK41686.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAK41686.1; Type=Frameshift; Note=SSO1459 and SSO8124 have been merged into one gene.; Evidence={ECO:0000305};
CC Sequence=AAK41687.1; Type=Frameshift; Note=SSO1459 and SSO8124 have been merged into one gene.; Evidence={ECO:0000305};
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DR EMBL; X71597; CAA50600.1; -; Genomic_DNA.
DR EMBL; AE006641; AAK41686.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AE006641; AAK41687.1; ALT_FRAME; Genomic_DNA.
DR PIR; G90303; G90303.
DR PIR; H90303; H90303.
DR PIR; S34120; S34120.
DR AlphaFoldDB; Q07635; -.
DR SMR; Q07635; -.
DR STRING; 273057.SSO1459; -.
DR PRIDE; Q07635; -.
DR EnsemblBacteria; AAK41686; AAK41686; SSO1459.
DR EnsemblBacteria; AAK41687; AAK41687; SSO8124.
DR KEGG; sso:SSO1459; -.
DR KEGG; sso:SSO8124; -.
DR PATRIC; fig|273057.12.peg.1489; -.
DR eggNOG; arCOG00329; Archaea.
DR HOGENOM; CLU_491446_0_0_2; -.
DR PhylomeDB; Q07635; -.
DR BRENDA; 2.7.7.7; 6163.
DR Proteomes; UP000001974; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IBA:GO_Central.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0006261; P:DNA-templated DNA replication; IBA:GO_Central.
DR Gene3D; 3.90.1600.10; -; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR Pfam; PF00136; DNA_pol_B; 1.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
PE 3: Inferred from homology;
KW DNA replication; DNA-binding; DNA-directed DNA polymerase;
KW Nucleotidyltransferase; Reference proteome; Transferase.
FT CHAIN 1..626
FT /note="DNA polymerase 2"
FT /id="PRO_0000046484"
FT CONFLICT 613
FT /note="K -> R (in Ref. 1; CAA50600)"
FT /evidence="ECO:0000305"
FT CONFLICT 617
FT /note="E -> D (in Ref. 1; CAA50600)"
FT /evidence="ECO:0000305"
FT CONFLICT 623
FT /note="M -> I (in Ref. 1; CAA50600)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 626 AA; 73535 MW; 21F47D2B047F03F5 CRC64;
MGRTQPSYTM AVNRELEKLE RIIERLHSPI LSLLLERVKE KVRYTQSASY DELVDPYNLV
YFALIWALAE ECEKMEEYVL DAYPIKGGVK LFLSNFKEKT IRTTFPVYTI TDNPDIVLQH
PEVRYYEKEK WKTLDGKEVK VYRFEVESLD AYYYMRKRLN VVNETPTVLS QTLYRLGIKP
FRRFRSSDDE FPKVTIARVV PLDWYGESLK GKVFEVKINN EVRRFYEKPE VEVDIAECLG
EACNYVKSNV KIRIEKKRSP VSAKGLIEWS FISLTPIHEI AYATIGKVLT INEAWVAFKR
RIIIPKVVPR VEKLRRLEDI MMVDKGGLIL FPQPGCYDNV YQVDFSSMYP SLIVKHNISA
ETVEACDDIK TELHSICLKE KGIIPEALQW LIERKSELKK IDEERAEAIK WILVASFGYL
GYRNSLFGKI EAYEMVTYLA RKTLRRTMEI AEEMGLRVLH GIIDSLVVKG DNVDKFIEKV
EKETGLRLDY KRYNWIIFTI TRNNTPYPTR YIANMNGEMI AKGLIRENMP NIVKSFLEDV
LREFSSAKTC SDVKKLRIRD LFEHYRKRAI NGEPIDYVIW IKDVPYVRGI KGFYEARLGY
MGRDVNYYIN YLKRVYEDVE EVMSRC