ID   DPOLA_BPPM6             Reviewed;         664 AA.
AC   P0DTK4;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=DNA-directed DNA polymerase {ECO:0000303|PubMed:34522950};
DE            EC=2.7.7.7 {ECO:0000269|PubMed:34522950};
GN   Name=gp62;
OS   Pseudomonas phage M6.
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Yuavirus.
OX   NCBI_TaxID=2911432;
OH   NCBI_TaxID=287; Pseudomonas aeruginosa.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16428425; DOI=10.1128/jb.188.3.1184-1187.2006;
RA   Kwan T., Liu J., Dubow M., Gros P., Pelletier J.;
RT   "Comparative genomic analysis of 18 Pseudomonas aeruginosa
RT   bacteriophages.";
RL   J. Bacteriol. 188:1184-1187(2006).
RN   [2]
RP   FUNCTION.
RX   PubMed=29555775; DOI=10.1073/pnas.1714812115;
RA   Lee Y.J., Dai N., Walsh S.E., Mueller S., Fraser M.E., Kauffman K.M.,
RA   Guan C., Correa I.R. Jr., Weigele P.R.;
RT   "Identification and biosynthesis of thymidine hypermodifications in the
RT   genomic DNA of widespread bacterial viruses.";
RL   Proc. Natl. Acad. Sci. U.S.A. 115:E3116-E3125(2018).
RN   [3]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=34522950; DOI=10.1093/nar/gkab781;
RA   Lee Y.J., Dai N., Mueller S.I., Guan C., Parker M.J., Fraser M.E.,
RA   Walsh S.E., Sridar J., Mulholland A., Nayak K., Sun Z., Lin Y.C.,
RA   Comb D.G., Marks K., Gonzalez R., Dowling D.P., Bandarian V., Saleh L.,
RA   Correa I.R., Weigele P.R.;
RT   "Pathways of thymidine hypermodification.";
RL   Nucleic Acids Res. 0:0-0(2021).
CC   -!- FUNCTION: DNA polymerase that replicates the viral genomic DNA
CC       (Probable). Also incorporates 5-hydroxymethyl-2'-deoxyuridine (5-hmdU)
CC       instead of dTMP into DNA during replication, as an early step in the
CC       pathway of thymidine hypermodifications of the viral genome
CC       (PubMed:34522950). As a final result of the pathway of
CC       hypermodification, 5-aminoethyl-2'-deoxyuridine (5-NedU) substitutes
CC       for about 30% of thymidines in the viral DNA (PubMed:34522950,
CC       PubMed:29555775). These modifications probably prevent degradation of
CC       viral genome by the host restriction-modification antiviral defense
CC       system (PubMed:34522950). {ECO:0000269|PubMed:29555775,
CC       ECO:0000269|PubMed:34522950, ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC         Evidence={ECO:0000269|PubMed:34522950};
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-A family. DpoZ
CC       subfamily. {ECO:0000305}.
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DR   EMBL; DQ163916; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; YP_001294570.1; NC_007809.1.
DR   PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE   1: Evidence at protein level;
KW   DNA replication; DNA-directed DNA polymerase; Host-virus interaction;
KW   Nucleotidyltransferase; Restriction-modification system evasion by virus;
KW   Transferase; Viral DNA replication.
FT   CHAIN           1..664
FT                   /note="DNA-directed DNA polymerase"
FT                   /id="PRO_0000456271"
SQ   SEQUENCE   664 AA;  74282 MW;  E200D5393C097FF5 CRC64;
     MSYLQPPLFT TVSSDWVAPD LSTLPSWEGA KRVAIDCETR DPDLRKLGPG AGRRPNSYIT
     GISFAIEDGP GGYLPIRHEG GGNLPLEGVL AYLRAQAKVF TGDLVGANLP YDLDFLAGDG
     IEFERVRYFR DIQIADPLIC ELHDSYSMQA IAERWGFHGK DEALLRAAAV DYGIDPKKDM
     WMLPAKFVGK YAEEDTRLPL NILRRQEREI DEQDLWGVYN LESKLLPILT GLRRRGVRID
     CDRLDMIERW ALEKETEALA QVRSITGHRI AVGDVWKPEV IAPALEHIGI KLNKTSQGKP
     NIDKELLGSI DHPVADLLER ARKVNKLRTT FASSVRDHMV NGRLHGTFNQ LRRQKDDESD
     GTAGAAYGRL SSEHPNLQQQ PARDEFAMMW RAIYLPEEGQ HWASNDYSQQ EPRMAVHYAC
     LAKDLIGHQA WLSAIEARDK YRNDPNTDNH QMMADMAGIK RKDAKEIYLG LSYGMGGAKM
     CRKLGLPTMM AVRGPRFQLF DVNSPEGQRL VAEGARRFEA AGPEGQALLD TFDHKVPFIK
     KLAKACEARA KAVGYITTLS GRRCRFPKDK DGNYDWTHKG LNRLIQGSSA DQTKMAMVAC
     AEAGLDIIIQ VHDEIAFSVH DMKEAAEAAH IMRTCTPLEL PSKVDVEIGQ SWGHSMGWDG
     NPPS