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DPOLA_STENO
ID   DPOLA_STENO             Reviewed;        1492 AA.
AC   Q94636;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=DNA polymerase alpha catalytic subunit;
DE            EC=2.7.7.7;
OS   Sterkiella nova (Ciliate) (Oxytricha nova).
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata; Spirotrichea;
OC   Stichotrichia; Sporadotrichida; Oxytrichidae; Stylonychinae; Sterkiella.
OX   NCBI_TaxID=200597;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8039700; DOI=10.1016/0378-1119(94)90373-5;
RA   Mansour S.J., Hoffman D.C., Prescott D.M.;
RT   "A gene-sized DNA molecule encoding the catalytic subunit of DNA polymerase
RT   alpha in the macronucleus of Oxytricha nova.";
RL   Gene 144:155-161(1994).
CC   -!- FUNCTION: Polymerase alpha in a complex with DNA primase is a
CC       replicative polymerase.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- MISCELLANEOUS: In eukaryotes there are five DNA polymerases: alpha,
CC       beta, gamma, delta, and epsilon which are responsible for different
CC       reactions of DNA synthesis.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
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DR   EMBL; U02001; AAA21587.1; -; Genomic_DNA.
DR   PIR; T18560; T18560.
DR   AlphaFoldDB; Q94636; -.
DR   SMR; Q94636; -.
DR   PRIDE; Q94636; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:1902975; P:mitotic DNA replication initiation; IEA:InterPro.
DR   CDD; cd05532; POLBc_alpha; 1.
DR   Gene3D; 1.10.132.60; -; 1.
DR   Gene3D; 1.10.3200.20; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.90.1600.10; -; 1.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR   InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR024647; DNA_pol_a_cat_su_N.
DR   InterPro; IPR042087; DNA_pol_B_thumb.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR038256; Pol_alpha_znc_sf.
DR   InterPro; IPR045846; POLBc_alpha.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   InterPro; IPR015088; Znf_DNA-dir_DNA_pol_B_alpha.
DR   Pfam; PF12254; DNA_pol_alpha_N; 1.
DR   Pfam; PF00136; DNA_pol_B; 1.
DR   Pfam; PF03104; DNA_pol_B_exo1; 1.
DR   Pfam; PF08996; zf-DNA_Pol; 1.
DR   PRINTS; PR00106; DNAPOLB.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase; Metal-binding;
KW   Nucleotidyltransferase; Nucleus; Transferase; Zinc; Zinc-finger.
FT   CHAIN           1..1492
FT                   /note="DNA polymerase alpha catalytic subunit"
FT                   /id="PRO_0000046434"
FT   ZN_FING         1314..1344
FT                   /note="CysA-type"
FT   REGION          34..112
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          162..195
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          210..234
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           1375..1398
FT                   /note="CysB motif"
FT   COMPBIAS        34..73
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        74..112
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        162..179
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        180..195
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         1314
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P13382"
FT   BINDING         1317
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P13382"
FT   BINDING         1341
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P13382"
FT   BINDING         1344
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P13382"
FT   BINDING         1375
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P13382"
FT   BINDING         1380
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P13382"
FT   BINDING         1393
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P13382"
FT   BINDING         1398
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P13382"
SQ   SEQUENCE   1492 AA;  171825 MW;  CEF02003A6DB279A CRC64;
     MIKHILQVEE TKKIFEEIDD NEYEKIQDSR KNDDFIVDDD GYGYRDHGGE IWDRDGDVEE
     VGKKKKKKQN NHDPNENIMN YMMPASTLKK KSNAASTNPS AQQKPKVSVE QSRDIMNNLF
     QQLDAKDVDE LEDVNEAKNM FVQEMNRPVA FNKEEDFNNR YSVTLESREE QERRRQSEQL
     KQQANIGQNQ SDVNPFSKKR KLDEFQQVQA NSYQSKQNSH SVSKSKPGDH EMANHADGVD
     LNLLAIDDTK MTDSHPSEII TQNQRASAVS SVNQQIMEST NGKNQLEKND TEWQQMKEKN
     AVFNQDLRMN DNALMSNQQD YPLPLNEDGT LSFYWIDAHE ENNGADLFVF GKIYQHEERK
     FVSCSIKVNG MQRELFVLPK MSGKSRAAMT TEEEKEQARK VMMELEGVRK RFPAITKWRC
     KPVTRKYAFE LPIQHGEHQF VKIKYDATFP SLPSTVQGNT FECIFGANQS MLESFILKRK
     IRGPCWMTIR NPQKVTDFRR TWCKQEILVS NPKDIEITLD DLNKTELPPL TSVTFAIKTC
     RSSQNTNEIA MLSCIVNENI SQEGPSKIDV HKSFTLLRKL DGKPMPIEYE RAFRDKKDSF
     IQFFQHERQL IEAFVAKIYQ LDPDLMVAHN LCGGMFDLLL ARIQMLKISH WSRIGRLKKN
     QIPNKKSDQS GANYGGSQWI PRQVTCGRLL VDTFLTAKEL IRETNYDLTH LAKVQLQKDR
     IDFDDDLLPT FYVQMAKLFQ LIDHTEKDAY LTLQLMNHLQ VIPLTKQLTN IAGNLWFRSL
     QNARAERNEM LLLHEFKKKK FVLPDKKQLN AKDLKKNMFA DEYEEGDGKT KGGKRKKAAY
     AGGLVIEPKA GFYDNIILLL DFNSLYPSII QEYNLCFTTV NRRPTKNFDG SEMKNQYKKG
     ENGEEEVDIE EADLPDKNVN LKDAVLPMVL RDLVQKRKAV KDKMKTEKDH VKLSQLEIRQ
     KAIKLTANSM YGCLGFGSSR FHAQAIAALI TRTGRETLLR TKDIAENKLG FNVVYGDTDS
     IMINTGSNQL QQALEMGKRL KGEVNCLYKC LEIEIDGVFK SLLLLKKKKY AALKYENFLS
     PAEVKVVQEM KGLDMVRRDW CPLSKRVGRY VLDQILSGKQ REEVVLNLNE FLSNIGNELK
     EGTIKLNEFI ITKQITKAIS DYNDIKGQPH VAVAKRLRDQ GKSENQLVNN FIPYVICQQT
     YGDTTKSSTA LSDKAYHPDE VISSRGKVTI DSDWYVSTQL LPPITRLIEH IEGIEVEFVA
     QCLGLDPKKY RYHSEKKNTD NPTDDPLIVS NPVLQTETER SLKNRTVAEL NIKCPHCAHN
     YHFPGILVPS SNNTELTGLA CVKCNQRIPD AYMLNRLNLF LKQLTALYYL GMKECKEPQC
     GMKTNQLLLN NKCIVKGCKG KMNSEYSELR INDTLRYLEG LFNVKKFLIE NEKYRKKYEK
     PELVPNFQSF KELQKKVESF MIRSGYNKVD LGNIFGFMSK GANPHQQKMS IF
 
 
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