ID   DPOL_ASFB7              Reviewed;        1211 AA.
AC   P42489;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   23-FEB-2022, sequence version 2.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=DNA polymerase beta {ECO:0000303|PubMed:8121806};
DE            EC=2.7.7.7;
GN   Name=DPOL; OrderedLocusNames=Ba71V-90; ORFNames=G1207R, G1211R;
OS   African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10498;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8293992; DOI=10.1016/0378-1119(93)90453-a;
RA   Rodriguez J.M., Yanez R.J., Rodriguez J.F., Vinuela E., Salas M.L.;
RT   "The DNA polymerase-encoding gene of African swine fever virus: sequence
RT   and transcriptional analysis.";
RL   Gene 136:103-110(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA   Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA   Rodriguez J.F., Vinuela E.;
RT   "Analysis of the complete nucleotide sequence of African swine fever
RT   virus.";
RL   Virology 208:249-278(1995).
RN   [3]
RP   IDENTIFICATION.
RX   PubMed=8121806; DOI=10.1093/nar/22.2.208;
RA   Martins A., Ribeiro G., Marques M.I., Costa J.V.;
RT   "Genetic identification and nucleotide sequence of the DNA polymerase gene
RT   of African swine fever virus.";
RL   Nucleic Acids Res. 22:208-213(1994).
RN   [4]
RP   INDUCTION, AND ALTERNATIVE INITIATION.
RX   PubMed=32075923; DOI=10.1128/jvi.00119-20;
RA   Cackett G., Matelska D., Sykora M., Portugal R., Malecki M., Baehler J.,
RA   Dixon L., Werner F.;
RT   "The African Swine Fever Virus Transcriptome.";
RL   J. Virol. 94:0-0(2020).
CC   -!- FUNCTION: DNA-directed DNA polymerase involved in viral DNA
CC       replication.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=1;
CC         IsoId=P42489-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=P42489-2; Sequence=VSP_061338;
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:32075923}.
CC   -!- MISCELLANEOUS: Consistent with its intracellular location, ASFV encodes
CC       its own replicative DNA polymerase and three base excision repair
CC       enzymes: a class II AP endonuclease, the repair polymerase Pol X, and
CC       an ATP-dependent DNA ligase.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
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DR   EMBL; L19042; AAA42690.1; -; Genomic_DNA.
DR   EMBL; U18466; AAA65319.1; -; Genomic_DNA.
DR   RefSeq; NP_042783.1; NC_001659.2.
DR   GeneID; 22220319; -.
DR   KEGG; vg:22220319; -.
DR   BRENDA; 2.7.7.7; 176.
DR   Proteomes; UP000000624; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.132.60; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.90.1600.10; -; 1.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR   InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR042087; DNA_pol_B_thumb.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF00136; DNA_pol_B; 1.
DR   Pfam; PF03104; DNA_pol_B_exo1; 1.
DR   PRINTS; PR00106; DNAPOLB.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   2: Evidence at transcript level;
KW   Alternative initiation; DNA replication; DNA-binding;
KW   DNA-directed DNA polymerase; Early protein; Nucleotidyltransferase;
KW   Reference proteome; Repeat; Transferase; Viral DNA replication.
FT   CHAIN           1..1211
FT                   /note="DNA polymerase beta"
FT                   /id="PRO_0000046502"
FT   REPEAT          1074..1077
FT                   /note="1"
FT   REPEAT          1078..1081
FT                   /note="2"
FT   REPEAT          1082..1085
FT                   /note="3"
FT   REPEAT          1086..1089
FT                   /note="4"
FT   REGION          1074..1089
FT                   /note="4 X 4 AA tandem repeats of [NK]-[P]-A-G"
FT   VAR_SEQ         1..4
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000269|PubMed:32075923"
FT                   /id="VSP_061338"
SQ   SEQUENCE   1211 AA;  139837 MW;  07D9053A90831798 CRC64;
     MISIMDRSEI VARENPVITQ RVTNLLQTNA PLLFMPIDIH EVRYGAYTLF MYGSLENGYK
     AEVRIENIPV FFDVQIEFND TNQLFLKSLL TAENIAYERL ETLTQRPVMG YREKEKEFAP
     YIRIFFKSLY EQRKAITYLN NMGYNTAADD TTCYYRMVSR ELKLPLTSWI QLQHYSYEPR
     GLVHRFSVTP EDLVSYQDDG PTDHSIVMAY DIETYSPVKG TVPDPNQAND VVFMICMRIF
     WIHSTEPLAS TCITMAPCKK SSEWTTILCS SEKNLLLSFA EQFSRWAPDI CTGFNDSRYD
     WPFIVEKSMQ HGILEEIFNK MSLFWHQKLD TILKCYYVKE KRVKISAEKS IISSFLHTPG
     CLPIDVRNMC MQLYPKAEKT SLKAFLENCG LDSKVDLPYH LMWKYYETRD SEKMADVAYY
     CIIDAQRCQD LLVRHNVIPD RREVGILSYT SLYDCIYYAG GHKVCNMLIA YAIHDEYGRI
     ACSTIARGKR EHGKYPGAFV IDPVKGLEQD KPTTGLDFAS LYPSLIMAYN FSPEKFVASR
     DEAKSLMAKG ESLHYVSFHF NNRLVEGWFV RHNNVPDKMG LYPKVLIDLL NKRTALKQEL
     KKLGEKKECI HESHPGFKEL QFRHAMVDAK QKALKIFMNT FYGEAGNNLS PFFLLPLAGG
     VTSSGQYNLK LVYNFVINKG YGIKYGDTDS LYITCPDSLY TEVTDAYLNS QKTIKHYEQL
     CHEKVLLSMK AMSTLCAEVN EYLRQDNGTS YLRMAYEEVL FPVCFTGKKK YYGIAHVNTP
     NFNTKELFIR GIDIIKQGQT KLTKTIGTRI MEESMKLRRP EDHRPPLIEI VKTVLKDAVV
     NMKQWNFEDF IQTDAWRPDK DNKAVQIFMS RMHARREQLK KHGAAASQFA EPEPGERFSY
     VIVEKQVQFD IQGHRTDSSR KGDKMEYVSE AKAKNLPIDI LFYINNYVLG LCARFINENE
     EFQPPDNVSN KDEYAQRRAK SYLQKFVQSI HPKDKSVIKQ GIVHRQCYKY VHQEIKKKIG
     IFADLYKEFF NNTTNPIESF IQSARFMIQY SDGEQKVNHS MKKMVEQRAT LASKPAGKPA
     GNPAGNPAGN ALMRAIFTQL ITEEKKIVQA LYNKGDAIHD LLTYIINNIN YKIATFQTKQ
     MLTFEFSSTH VELLLKLNKT WLILAGIHVA KKHLQALLDS YNNEPPSRTF IQQAIEEECG
     SIKPSCYDFI S