ID   DPOL_ASFK5              Reviewed;        1196 AA.
AC   P0C974;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   23-FEB-2022, entry version 44.
DE   RecName: Full=DNA polymerase beta;
DE            EC=2.7.7.7 {ECO:0000250|UniProtKB:P42489};
GN   OrderedLocusNames=Ken-102;
OS   African swine fever virus (isolate Pig/Kenya/KEN-50/1950) (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=561445;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH   NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH   NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Kutish G.F., Rock D.L.;
RT   "African swine fever virus genomes.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-directed DNA polymerase involved in viral DNA
CC       replication. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000305}.
CC   -!- MISCELLANEOUS: Consistent with its intracellular location, ASFV encodes
CC       its own replicative DNA polymerase and three base excision repair
CC       enzymes: a class II AP endonuclease, the repair polymerase Pol X, and
CC       an ATP-dependent DNA ligase.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
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DR   EMBL; AY261360; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   SMR; P0C974; -.
DR   Proteomes; UP000000861; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.132.60; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.90.1600.10; -; 1.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR   InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR042087; DNA_pol_B_thumb.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF00136; DNA_pol_B; 1.
DR   Pfam; PF03104; DNA_pol_B_exo1; 1.
DR   PRINTS; PR00106; DNAPOLB.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase;
KW   Nucleotidyltransferase; Transferase; Viral DNA replication.
FT   CHAIN           1..1196
FT                   /note="DNA polymerase beta"
FT                   /id="PRO_0000373073"
SQ   SEQUENCE   1196 AA;  138462 MW;  4C0964677E6A42F4 CRC64;
     MDRSEIVARE NPVITQRVTN LLRTNAPLLF MPIDIHEVRY GAYMLFMYGS LENGYKAEVR
     IENIPVFFDV QIESDNTNQL FLKSLLAAEN ITYERLETLT QRPVMGYREK EKEFAPYIRI
     FFKSLYERRK AITYLNNMGY NTAADDTTCY YRMVSRELKL PLTSWIQLQH YSYEPRGLVH
     RFSVTPEDLV SYQDDGPTDH SIVMAYDIET YSPVKGTVPD PNQANDVVFM ICMRIFWIHS
     TEPLASTCIT MAPCKKSPEW TTIVCSSEKN LLLSFAEQFS RWAPDICTGF NDSRYDWPFI
     VEKSMQHDIL EEVFNKMSLF WPQKLDTILK CYYVKEKRVK ISAEKSIISS FLHTPGCLPM
     DVRNMCMQLY PKAEKTSLKA FLENCGLDSK VDLPYHLMWK YYETRDSEKM ADVAYYCIID
     AQRCQDLLVR HNVIPDRREV GILSYTSLYD CIYYAGGHKV CNMLIAYAIH DEYGRIACST
     IARGKREHGK YPGAFVIDPV KGLEQDKPTT GLDFASLYPS LIMAYNFSPE KFVASRDEAN
     SLMAKGESLH YVSFHFNNRL VEGWFVRHNN VPDKMGLYPK VLIDLLNKRT ALKQELKKLG
     EKKECIHESH PGFKELQFRH AMVDAKQKAL KIFMNTFYGE AGNNLSPFFL LPLAGGVTSS
     GQYNLKLVYN FVINKGYGIK YGDTDSLYIT CPDSLYTEVT DAYLNSQKTI KHYEQLCHEK
     VLLSMKAMST LCAEVNEYLR QDNGTSYLRM AYEEVLFPVC FTGKKKYYGI AHVNTPNFNT
     KELFIRGIDI IKQGQTKLTK TIGTRIMEES MKLRRPEDHR PPLIEIVKTV LKDAVVNMKQ
     WNFEDFIQTD AWRPDKDNKA VQIFMSRMHA RREQLKKHGA AATSQFAEPE PGERFSYVIV
     EKQVQFDIQG HRTDTTRKGD KMEYVSEAKA KNLPIDILFY INNYVLGLCA RFINENEEFQ
     PPGNVSNKDE YAQRRAKSYL QKFVQSIHPK DKSVIKQGIV HRQCYKYVHQ EIKKKIGIFA
     DLYKEFFNNT TNPIESFIQS TQFMIHYFDE EQKVNHSMKK MVEQHAALAG NPAGNALMRA
     IFTQLIVEEK KIVQALYNKG DEIHDLLTYI INNINYKIAT FQTKQMLTFE LSSTHVELLL
     KLNKTWLILV GIHVAKKHLH VLLGLSNNEP PSKTFIQQAI EEECGSIKPS CYDFIS