ID   DPOL_ASFM2              Reviewed;        1206 AA.
AC   P0C972;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=DNA polymerase beta {ECO:0000250|UniProtKB:P42489};
DE            EC=2.7.7.7;
GN   OrderedLocusNames=Mal-098;
OS   African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10500;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH   NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH   NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Kutish G.F., Rock D.L.;
RT   "African swine fever virus genomes.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-directed DNA polymerase involved in viral DNA
CC       replication. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000305}.
CC   -!- MISCELLANEOUS: Consistent with its intracellular location, ASFV encodes
CC       its own replicative DNA polymerase and three base excision repair
CC       enzymes: a class II AP endonuclease, the repair polymerase Pol X, and
CC       an ATP-dependent DNA ligase.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
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DR   EMBL; AY261361; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   SMR; P0C972; -.
DR   Proteomes; UP000000860; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.132.60; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.90.1600.10; -; 1.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR   InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR042087; DNA_pol_B_thumb.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF00136; DNA_pol_B; 1.
DR   Pfam; PF03104; DNA_pol_B_exo1; 1.
DR   PRINTS; PR00106; DNAPOLB.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase;
KW   Nucleotidyltransferase; Repeat; Transferase; Viral DNA replication.
FT   CHAIN           1..1206
FT                   /note="DNA polymerase beta"
FT                   /id="PRO_0000373071"
FT   REPEAT          1071..1074
FT                   /note="1"
FT   REPEAT          1075..1078
FT                   /note="2"
FT   REPEAT          1079..1082
FT                   /note="3"
FT   REPEAT          1083..1086
FT                   /note="4"
FT   REGION          1071..1086
FT                   /note="4 X 4 AA tandem repeats of A-G-[NK]-[PA]"
SQ   SEQUENCE   1206 AA;  139527 MW;  CF9392A4A672496E CRC64;
     MDRSEIVARE NPVITQRVTN LLRTNAPLLF MPIDIHEVRY GAYMLFMYGS LENGYKAEVR
     IENIPVFFDV QIESDNTNQL FLKSLLTAEN ITYERLESIT QRPVMGYREK EKEFAPYIRI
     FFKSLYERRK AITYLNNMGY NTAADDTTCY YRMVSRELKL PLTSWIQLQN YSYEPRGLVH
     RFSVTPDDLV SYQDDGLTDH SIVMAYDIET YSPVKGTVPD PNQANDVVFM ICMHIFWIHS
     TEPLASTCIT MVPCKKSSEW TTIVCSSEKN LLLSFAEQFS RWVPDICTGF NDSRYDWPFI
     VEKSMQHGIL EEIFNKMSLF WNQKLDTILK CYYVKEKRVK ISAEKSIISS FLHTPGCLPM
     DVRNMCMQIY PKAEKTSLKA FLENCGLDSK VDLPYHLMWK YYETRDSEKM ADVAYYCIID
     AQRCQDLLVR HNVIPDRREV GILSYTSLYD CIYYAGGHKV CNMLIAYAIH DEYGRIACST
     IARGKREHGK YPGAFVIDPI KGLEQDKPTT GLDFASLYPS LIMAYNFSPE KFVASRDEAN
     NLMAKGESLH YVSFHFNNRL VEGWFVRHNN VPDKMGLYPK VLIDLLNKRT ILKQELKKLG
     EKKECIHESH PGFKELQFRH AMVDAKQKAL KIFMNTFYGE AGNNLSPFFL LPLAGGVTSS
     GQYNLKLVYN FVINKGYGIK YGDTDSLYIT CPDSLYTEVT EAYLNSQKTI KHYEQLCHEK
     VLLSMKAMST LCAEVNEYLR QDNGTSYLRM AYEEVLFPVC FTGKKKYYGI AHVNTPNFNT
     KELFIRGIDI IKQGQTKLTK TIGMRIMEES MKLRRPEDHR PPLIEIVKTV LKDAVVNMKQ
     WNFEDFIQTD AWRPDKDNKA VQIFMSRMHA RREQLKKHGA AASQFAEPEP GERFSYVIVE
     KQVQFDIQGH RTDTSRKGDK MEYVSEAKAK NLPIDILFYI NNYVLGLCAR FINENEEFQP
     PDNVSNKDEY AQRRAKSYLQ KFVQSIHPKD KSVIKQGIVH RQCYKYVHQE IKKKIGIFAD
     LYKEFFNNTT NPIESFIQST QFMIQYSDEE QKVNHSVKKM VEQHATASNR AGNPAGNPAG
     NPAGNALMRA IFTQLITEEK KIVQALYSKG DEIHDLLTYI INNINYKIAT FQTKQMLTFE
     LSSTHVELLL KLNKTWLILT GIHVAKKHLQ ALLDSYNNEP PSKTFIQQAI KEECSSIKPS
     CYDFIS