ID   DPOL_BPAPS              Reviewed;         993 AA.
AC   Q9T1Q3;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Probable DNA polymerase;
DE            EC=2.7.7.7 {ECO:0000250|UniProtKB:P00581};
DE            EC=3.1.11.- {ECO:0000250|UniProtKB:P00581};
DE   AltName: Full=P45;
GN   Name=45;
OS   Acyrthosiphon pisum secondary endosymbiont phage 1 (Bacteriophage APSE-1).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Podoviridae; Sendosyvirus.
OX   NCBI_TaxID=2682836;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10489345; DOI=10.1006/viro.1999.9902;
RA   van der Wilk F., Dullemans A.M., Verbeek M., van den Heuvel J.F.J.M.;
RT   "Isolation and characterization of APSE-1, a bacteriophage infecting the
RT   secondary endosymbiont of acyrthosiphon pisum.";
RL   Virology 262:104-113(1999).
CC   -!- FUNCTION: Replicates viral genomic DNA. This polymerase possesses two
CC       enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic
CC       activity that degrades single-stranded DNA in the 3'-5' direction.
CC       {ECO:0000250|UniProtKB:P00581}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC         Evidence={ECO:0000250|UniProtKB:P00581};
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-A family. {ECO:0000305}.
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DR   EMBL; AF157835; AAF03988.1; -; Genomic_DNA.
DR   RefSeq; NP_051006.1; NC_000935.1.
DR   GeneID; 1262339; -.
DR   KEGG; vg:1262339; -.
DR   Proteomes; UP000000853; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR   GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR   InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR036844; Hint_dom_sf.
DR   InterPro; IPR030934; Intein_C.
DR   InterPro; IPR006141; Intein_N.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   Pfam; PF00476; DNA_pol_A; 1.
DR   SMART; SM00482; POLAc; 1.
DR   SUPFAM; SSF51294; SSF51294; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 2.
DR   PROSITE; PS50818; INTEIN_C_TER; 1.
DR   PROSITE; PS50817; INTEIN_N_TER; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-directed DNA polymerase; Hydrolase;
KW   Nucleotidyltransferase; Reference proteome; Transferase;
KW   Viral DNA replication.
FT   CHAIN           1..993
FT                   /note="Probable DNA polymerase"
FT                   /id="PRO_0000101262"
SQ   SEQUENCE   993 AA;  111764 MW;  82D134031549F04D CRC64;
     MQNLLFCDLE TYSDIPINCG THRYAENAEI LLFAYAYNHA PVKVWDVTQD KTMPADLKAY
     LDDSEILTVW HNGGMFDTVI LKRVLNIDLP LSRVHDTLVQ ALAHGLPGAL GLLCDIFNVN
     SDKAKDKEGK ALISLLCKPR PKNSKIQRAT ALTHAEEWQR FKDYAGSDIL AMREIYQHLP
     NWNMNVHETE LWQLDQKINR RGMCMDVELA KSALTAVENE QKRLSTVTQQ LTDNAVQNAT
     QRDALLQHIA SAFGITLPDM QASTLQRRIN DPDIPPALRE LLSVRLQSCT TSTSKYKALL
     KSVSADGRLR GTKQFCGASR TGRWAGRVFQ PDNLPRPTLD PNTIDNGIEA LKAGCAELIC
     DDIMQLTSSA LRGCIIAPPG KKLVISDLSN IEGRMLAWLA GENWKVNAFS EFDNGKGDDL
     YKLAYARAFN LLPEDVTKEQ RQIGKVMELG LGYGGGVAAF LTFALAYGLD LDELAEAALP
     NIPPGVKREA MRWYQKSVET DKTYGLSEKI FVTCDSLKRM WRNAHPQTVS FWYDIEDAVK
     QAIQSPGIAF KCRKLSVRRD KSWLRICLPS GRSLCYPSAR IENGQITYMG TNPYSRKWER
     LKTYGGKSCL AKGTLVLTIT GWMPIEIVSQ DAYVWDGIEW VRTDGSVFNG NQEVIQAYGV
     GMTADHQVLT EKGWKSASQS KRYNRSSCRL PDGYKLPRFR RKEINLESTL HLWTRNNHSS
     NRITKTKKTR YNCLLRMPKG TNNIMQKPKA RNVKTPRFCC MEQHVSQMYS PFPQSMVKLW
     WSGNNGLQTL AKKFQQFLGR HGQDIPTRLI FRSHQQQCRL PPQKLPLGYV ASTSSKYSTS
     TIRANSPRHN EYTGISSPNR DCSKHALLSP GKKGKSSTTS GAPKHIAEVY DLINCGPRNR
     FVIATPDGPL IVHNCENICQ AAARDVLAYN MPPIEKAGYE IVLTVHDEII SEAPDTPQFS
     AEGLSKLLSF NSDWAWDLPL SANGFETYRY RKE