ID   DPOL_BPML5              Reviewed;         595 AA.
AC   Q05254;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=DNA polymerase;
DE            EC=2.7.7.7;
DE            EC=3.1.11.- {ECO:0000250|UniProtKB:P06225};
GN   Name=44;
OS   Mycobacterium phage L5 (Mycobacteriophage L5).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Fromanvirus.
OX   NCBI_TaxID=31757;
OH   NCBI_TaxID=1763; Mycobacterium.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8459766; DOI=10.1111/j.1365-2958.1993.tb01131.x;
RA   Hatfull G.F., Sarkis G.J.;
RT   "DNA sequence, structure and gene expression of mycobacteriophage L5: a
RT   phage system for mycobacterial genetics.";
RL   Mol. Microbiol. 7:395-405(1993).
CC   -!- FUNCTION: Replicates viral genomic DNA. This polymerase possesses two
CC       enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic
CC       activity that degrades single-stranded DNA in the 3'-5' direction.
CC       {ECO:0000250|UniProtKB:P06225}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-A family. {ECO:0000305}.
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DR   EMBL; Z18946; CAA79420.1; -; Genomic_DNA.
DR   PIR; S30989; S30989.
DR   RefSeq; NP_039708.1; NC_001335.1.
DR   SMR; Q05254; -.
DR   GeneID; 2942951; -.
DR   KEGG; vg:2942951; -.
DR   Proteomes; UP000002123; Genome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IEA:InterPro.
DR   GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR   InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR   InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR002298; DNA_polymerase_A.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   PANTHER; PTHR10133; PTHR10133; 1.
DR   Pfam; PF00476; DNA_pol_A; 1.
DR   Pfam; PF01612; DNA_pol_A_exo1; 1.
DR   SMART; SM00474; 35EXOc; 1.
DR   SMART; SM00482; POLAc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease;
KW   Hydrolase; Nuclease; Nucleotidyltransferase; Reference proteome;
KW   Transferase; Viral DNA replication.
FT   CHAIN           1..595
FT                   /note="DNA polymerase"
FT                   /id="PRO_0000101264"
FT   DOMAIN          1..212
FT                   /note="3'-5' exonuclease"
FT   REGION          213..595
FT                   /note="Polymerase"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   595 AA;  66221 MW;  EB4AA7DBFBB4DCC6 CRC64;
     MIELRHEVQG DLVTVNVVET PEDLEGFRNF IRAHLNCLAV DTETTGLDIY SDTFECRLVQ
     FGTQDEAWVV PVELGDVFIE DVRIAIGALK RMVLQNASFD LQVLDQCFGI EMEGLWPRVL
     DTQILAKLVD PRPFEAGGFG HSLEELIAKF ISEDQAENVK KLMAKLAAEH KTTKAKIWST
     IDLFHPEYLL YAGMDTIFTA RVCKSLTPLV PDVSRSLVPY EHKISEICSY IDRQGFLLDV
     EYSRSLAEKW LADQEVWEAI AFTEYGVEKV NSTEDLAEGL EEMGVKITGR TETGKRQVNA
     ALLDKLVEDG NELAAIAQEA KKLGKWRKTW VQKFIDTRDS EDRCHTFINP LQARTSRMSI
     TGIPAQTLPS SDWIVRRCFI AEPGDVMASV DYQAQELRVL AALSGDRNMI EAFENGADLH
     QMTADAAQVP RKVGKTANFQ KVYGGGAKAL AEAVGISIPV AKRVHEAFSA TYPGVERLSK
     KLAMEAGRNG YIVNAMGRRL PVDSSRTYSA LNYMIQSSSR DVTCRALIRL HEAGYTPYLR
     LPIHDEIVAS LPASEAERAA AHIGHLMQEQ MGPVLVGTDP EVGKRSWGSL YGADY