DPOL_GPCMV
ID DPOL_GPCMV Reviewed; 1117 AA.
AC Q69025; E9RH70;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2013, sequence version 3.
DT 29-SEP-2021, entry version 96.
DE RecName: Full=DNA polymerase;
DE EC=2.7.7.7;
GN ORFNames=UL54;
OS Guinea pig cytomegalovirus (strain 22122) (GPCMV).
OC Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC Herpesvirales; Herpesviridae; Betaherpesvirinae; Quwivirus.
OX NCBI_TaxID=103920;
OH NCBI_TaxID=10141; Cavia porcellus (Guinea pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=22122/UMN;
RX PubMed=9049389; DOI=10.1099/0022-1317-76-7-1827;
RA Schleiss M.R.;
RT "Sequence and transcriptional analysis of the guinea-pig cytomegalovirus
RT DNA polymerase gene.";
RL J. Gen. Virol. 76:1827-1833(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION.
RC STRAIN=22122/UMN;
RX PubMed=19014498; DOI=10.1186/1743-422x-5-139;
RA Schleiss M.R., McGregor A., Choi K.Y., Date S.V., Cui X., McVoy M.A.;
RT "Analysis of the nucleotide sequence of the guinea pig cytomegalovirus
RT (GPCMV) genome.";
RL Virol. J. 5:139-139(2008).
RN [3]
RP SEQUENCE REVISION.
RC STRAIN=22122;
RX PubMed=23516193; DOI=10.1128/genomea.00054-13;
RA Yang D., Tamburro K., Dittmer D., Cui X., McVoy M.A.,
RA Hernandez-Alvarado N., Schleiss M.R.;
RT "Complete genome sequence of pathogenic Guinea pig cytomegalovirus from
RT salivary gland homogenates of infected animals.";
RL Genome Announc. 1:E0005413-E0005413(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=22122/ATCC-P5;
RX PubMed=21270288; DOI=10.1099/vir.0.027789-0;
RA Kanai K., Yamada S., Yamamoto Y., Fukui Y., Kurane I., Inoue N.;
RT "Re-evaluation of the genome sequence of guinea pig cytomegalovirus.";
RL J. Gen. Virol. 92:1005-1020(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC -!- SUBCELLULAR LOCATION: Host nucleus.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
CC -!- CAUTION: The strain used in PubMed:9049389 and PubMed:19014498 had been
CC extensively passaged and contains genetic alterations. A correct
CC sequence has thus been sequenced in PubMed:23516193 by direct
CC sequencing of salivary gland homogenates. {ECO:0000305}.
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DR EMBL; AB592928; BAJ78522.1; -; Genomic_DNA.
DR EMBL; KC503762; AGE11533.1; -; Genomic_DNA.
DR RefSeq; YP_007417829.1; NC_020231.1.
DR SMR; Q69025; -.
DR PRIDE; Q69025; -.
DR GeneID; 14536656; -.
DR KEGG; vg:14536656; -.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR Gene3D; 1.10.132.60; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR Gene3D; 3.90.1600.10; -; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR042087; DNA_pol_B_thumb.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF00136; DNA_pol_B; 1.
DR Pfam; PF03104; DNA_pol_B_exo1; 1.
DR PRINTS; PR00106; DNAPOLB.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE 3: Inferred from homology;
KW DNA replication; DNA-binding; DNA-directed DNA polymerase; Host nucleus;
KW Nucleotidyltransferase; Transferase; Viral DNA replication.
FT CHAIN 1..1117
FT /note="DNA polymerase"
FT /id="PRO_0000046506"
FT REGION 591..621
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 595..621
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1117 AA; 125316 MW; B8B0D33F2B580E06 CRC64;
MSAPVFFNPY LCGGAARRRN GCSTVDSRRG NGPTKKGKKS FLQVVPRGVI YDGEKGLIKK
VTQHPPRMFY NNVQYLLEPQ MSWPTLPCRE TCRVGCGREQ PLRFHTFDQI DSTVYADSVE
QIFLGYRRHV VPCGNVIRMF GRTCDGSSVC VNVFGQPSYF YCEYDGSEGY LDNYLSTVLK
ETEDVTKIVF TLDAQRVHKY SLFGYNTKYI ENLYRVTLNN WPVCKRLAQN LQSRGLRVYE
AGVDPVARFC VDRKIPSFGW CVIKRFYARS SGLASFCDIE IDCEIGDVEA DDSDMSWPEY
RCASFDIECM SGGDRFPDSS MVDDIVIQIS VICYAVGRSG AESDGVSGAE AAVREHQHLF
TLGPCAPIPG THVYEFPSEY ELLLGFFIFF KAYSPDILTG YNINLFDIKY LLQRMEKIYH
ANVSEFTKLR FGGRFSIYVP VGNKPRNASS ASIKVHCTGT VVLDMYPVCV AKTSAPNYKL
ETMAEMYLNE HKDDLSYKEI PPTFLANDNG RAVVGRYCIK DALLVKRLFE KLNYHYEAAS
VARLARIPLR SVIFEGQQIR IYSCILEEAG ERNMILPSFL TAKRPGELAT ESSPVASFEE
DSEQTSDSSL GEVSSQGSSD GGVGYQGATV LEPDVGFYDT PVAVFDFASL YPSIIMRHNL
CYSTYLPLGR DDGLSDDDVF LLEFDDGTRY GFVREHVRKS ILGELLARWL AKRKSVRKVL
AECQDEVEKL ILDKYQLALK VTCNAFYGFT GVSSGMMPCL PIAAAITRIG RDMLMSVVDY
VNTYMGHAEF WLRYLGEEDL TGDALNVKVI YGDTDSVFVI CGGVKCGSVL EHGEAIAGHI
TRALFREPIK LEFEKVFVNL MMICKKRYVG RIYGQTKLSM KGIELVRKTA CEYVKSTVRN
VLNMIFFEDD VSAGAVELSR MTMDDVKRHG VPSGFYRIVE ALSNARDELY LNRVDVKKLV
LSASLSQEVS AYKQQNLPHL RVIQRLAARR EELPSVGDRV PYVLIAPPPG SSKNVPNYEI
SEDPGYVIEH KLPVNGEKYF EHVVKTVTNV LGPIIPKDCA RKEKFLSYVL PQRVYVSRPF
MPYACAANEL VVACDDGGVM MGVYGIKPVM CGVSVTN
