DPOL_METJA
ID DPOL_METJA Reviewed; 1634 AA.
AC Q58295;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-1998, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=DNA polymerase;
DE EC=2.7.7.7;
DE Contains:
DE RecName: Full=Mja pol-1 intein;
DE Contains:
DE RecName: Full=Mja pol-2 intein;
GN Name=pol; OrderedLocusNames=MJ0885;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC -!- PTM: This protein undergoes a protein self splicing that involves a
CC post-translational excision of the intervening region (intein) followed
CC by peptide ligation. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
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DR EMBL; L77117; AAB98889.1; -; Genomic_DNA.
DR PIR; E64410; E64410.
DR AlphaFoldDB; Q58295; -.
DR SMR; Q58295; -.
DR STRING; 243232.MJ_0885; -.
DR PRIDE; Q58295; -.
DR EnsemblBacteria; AAB98889; AAB98889; MJ_0885.
DR KEGG; mja:MJ_0885; -.
DR eggNOG; arCOG00328; Archaea.
DR eggNOG; arCOG00329; Archaea.
DR eggNOG; arCOG03145; Archaea.
DR HOGENOM; CLU_000203_6_4_2; -.
DR InParanoid; Q58295; -.
DR OMA; YAHNSYY; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IBA:GO_Central.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0006261; P:DNA-templated DNA replication; IBA:GO_Central.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR Gene3D; 1.10.132.60; -; 1.
DR Gene3D; 3.10.28.10; -; 2.
DR Gene3D; 3.30.420.10; -; 1.
DR Gene3D; 3.90.1600.10; -; 2.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR042087; DNA_pol_B_thumb.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR041005; PI-TkoII_IV.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF00136; DNA_pol_B; 3.
DR Pfam; PF03104; DNA_pol_B_exo1; 1.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR Pfam; PF18714; PI-TkoII_IV; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00305; HintC; 2.
DR SMART; SM00306; HintN; 2.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF51294; SSF51294; 2.
DR SUPFAM; SSF53098; SSF53098; 1.
DR SUPFAM; SSF55608; SSF55608; 2.
DR SUPFAM; SSF56672; SSF56672; 3.
DR TIGRFAMs; TIGR01443; intein_Cterm; 2.
DR TIGRFAMs; TIGR01445; intein_Nterm; 1.
DR PROSITE; PS00116; DNA_POLYMERASE_B; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 2.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 2.
DR PROSITE; PS50817; INTEIN_N_TER; 2.
PE 3: Inferred from homology;
KW Autocatalytic cleavage; DNA replication; DNA-binding;
KW DNA-directed DNA polymerase; Nucleotidyltransferase; Protein splicing;
KW Reference proteome; Repeat; Transferase.
FT CHAIN 1..425
FT /note="DNA polymerase, 1st part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000007317"
FT CHAIN 426..794
FT /note="Mja pol-1 intein"
FT /evidence="ECO:0000255"
FT /id="PRO_0000007318"
FT CHAIN 795..882
FT /note="DNA polymerase, 2nd part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000007319"
FT CHAIN 883..1358
FT /note="Mja pol-2 intein"
FT /evidence="ECO:0000255"
FT /id="PRO_0000007320"
FT CHAIN 1359..1634
FT /note="DNA polymerase, 3rd part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000007321"
FT DOMAIN 552..693
FT /note="DOD-type homing endonuclease 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00273"
FT DOMAIN 1163..1295
FT /note="DOD-type homing endonuclease 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00273"
SQ SEQUENCE 1634 AA; 191710 MW; 84A1FAFAB1F97DDD CRC64;
MGMSMGKIKI DALIDNTYKT IEDKAVIYLY LINSILKDRD FKPYFYVELH KEKVENEDIE
KIKEFLLKND LLKFVENIEV VKKIILRKEK EVIKIIATHP QKVPKLRKIK ECEIVKEIYE
HDIPFAKRYL IDNEIIPMTY WDFENKKPVS IEIPKLKSVA FDMEVYNRDT EPNPERDPIL
MASFWDENGG KVITYKEFNH PNIEVVKNEK ELIKKIIETL KEYDVIYTYN GDNFDFPYLK
ARAKIYGIDI NLGKDGEELK IKRGGMEYRS YIPGRVHIDL YPISRRLLKL TKYTLEDVVY
NLFGIEKLKI PHTKIVDYWA NNDKTLIEYS LQDAKYTYKI GKYFFPLEVM FSRIVNQTPF
EITRMSSGQM VEYLLMKRAF KENMIVPNKP DEEEYRRRVL TTYEGGYVKE PEKGMFEDII
SMDFRCHPKG TKVVVKGKGI VNIEDVKEGN YVLGIDGWQK VKKVWKYEYE GELINVNGLK
CTPNHKIPLR YKIKHKKINK NDYLVRDIYA KSLLTKFKGE GKLILCKDFE TIGNYEKYIN
DMDEDFILKS ELIGILLAEG HLLRRDIEYF DSSRGKKRIS HQYRVEITVN EDEKDFIEKI
KYIFKKLFNY ELYVRRKKGT KAITLGCAKK DIYLKIEEIL KNKEKYLPNA ILRGFFEGDG
YVNTVRRAVV VNQGTNNYDK IKFIASLLDR LGIKYSFYTY SYEERGKKLK RYVIEIFSKG
DLIKFSILIS FISRRKNNLL NEIIRQKTLY KIGDYGFYDL DDVCVSLESY KGEVYDLTLE
GRPYYFANGI LTHNSLYPSI IISYNISPDT LDCECCKDVS EKILGHWFCK KKEGLIPKTL
RNLIERRINI KRRMKKMAEI GEINEEYNLL DYEQKSLKIL ANSILPDEYL TIIEEDGIKV
VKIGEYIDDL MRKHKDKIKF SGISEILETK NLKTFSFDKI TKKCEIKKVK ALIRHPYFGK
AYKIKLRSGR TIKVTRGHSL FKYENGKIVE VKGDDVRFGD LIVVPKKLTC VDKEVVINIP
KRLINADEEE IKDLVITKHK DKAFFVKLKK TLEDIENNKL KVIFDDCILY LKELGLIDYN
IIKKINKVDI KILDEEKFKA YKKYFDTVIE HGNFKKGRCN IQYIKIKDYI ANIPDKEFED
CEIGAYSGKI NALLKLDEKL AKFLGFFVTR GRLKKQKLKG ETVYEISVYK SLPEYQKEIA
ETFKEVFGAG SMVKDKVTMD NKIVYLVLKY IFKCGDKDKK HIPEELFLAS ESVIKSFLDG
FLKAKKNSHK GTSTFMAKDE KYLNQLMILF NLVGIPTRFT PVKNKGYKLT LNPKYGTVKD
LMLDEVKEIE AFEYSGYVYD LSVEDNENFL VNNIYAHNSV YGYLAFPRAR FYSRECAEIV
TYLGRKYILE TVKEAEKFGF KVLYIDTDGF YAIWKEKISK EELIKKAMEF VEYINSKLPG
TMELEFEGYF KRGIFVTKKR YALIDENGRV TVKGLEFVRR DWSNIAKITQ RRVLEALLVE
GSIEKAKKII QDVIKDLREK KIKKEDLIIY TQLTKDPKEY KTTAPHVEIA KKLMREGKRI
KVGDIIGYII VKGTKSISER AKLPEEVDID DIDVNYYIDN QILPPVLRIM EAVGVSKNEL
KKEGAQLTLD KFFK
