DPOL_NPVAC
ID DPOL_NPVAC Reviewed; 984 AA.
AC P18131;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 23-FEB-2022, entry version 104.
DE RecName: Full=DNA polymerase;
DE EC=2.7.7.7;
GN Name=POL; ORFNames=ORF65;
OS Autographa californica nuclear polyhedrosis virus (AcMNPV).
OC Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus.
OX NCBI_TaxID=46015;
OH NCBI_TaxID=7088; Lepidoptera (butterflies and moths).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=L1;
RX PubMed=3059678;
RA Tomalski M.D., Wu J.G., Miller L.K.;
RT "The location, sequence, transcription, and regulation of a baculovirus DNA
RT polymerase gene.";
RL Virology 167:591-600(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C6;
RX PubMed=8030224; DOI=10.1006/viro.1994.1380;
RA Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., Possee R.D.;
RT "The complete DNA sequence of Autographa californica nuclear polyhedrosis
RT virus.";
RL Virology 202:586-605(1994).
RN [3]
RP FUNCTION.
RX PubMed=15582664; DOI=10.1016/j.virol.2004.10.024;
RA Vanarsdall A.L., Okano K., Rohrmann G.F.;
RT "Characterization of the replication of a baculovirus mutant lacking the
RT DNA polymerase gene.";
RL Virology 331:175-180(2005).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=25008932; DOI=10.1128/jvi.01167-14;
RA Feng G., Krell P.J.;
RT "Autographa californica multiple nucleopolyhedrovirus DNA polymerase C
RT terminus is required for nuclear localization and viral DNA replication.";
RL J. Virol. 88:10918-10933(2014).
CC -!- FUNCTION: Replicates the viral genome, host DNA polymerases cannot
CC substitute for the viral enzyme in this process.
CC {ECO:0000269|PubMed:15582664}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:25008932}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
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DR EMBL; M20744; AAA46692.1; -; Genomic_DNA.
DR EMBL; L22858; AAA66695.1; -; Genomic_DNA.
DR PIR; A31832; DJNVCP.
DR RefSeq; NP_054095.1; NC_001623.1.
DR SMR; P18131; -.
DR GeneID; 1403898; -.
DR KEGG; vg:1403898; -.
DR Proteomes; UP000008292; Genome.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR GO; GO:0019079; P:viral genome replication; IDA:UniProtKB.
DR Gene3D; 1.10.132.60; -; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR Gene3D; 3.90.1600.10; -; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR042087; DNA_pol_B_thumb.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF00136; DNA_pol_B; 1.
DR Pfam; PF03104; DNA_pol_B_exo1; 1.
DR PRINTS; PR00106; DNAPOLB.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
DR PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE 3: Inferred from homology;
KW DNA replication; DNA-binding; DNA-directed DNA polymerase; Early protein;
KW Host nucleus; Nucleotidyltransferase; Reference proteome; Transferase;
KW Viral DNA replication.
FT CHAIN 1..984
FT /note="DNA polymerase"
FT /id="PRO_0000046526"
FT REGION 804..827
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:25008932"
FT REGION 939..948
FT /note="Monopartite nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:25008932"
FT REGION 943..969
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 949..964
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 830
FT /note="R -> W (in Ref. 1; AAA46692)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 984 AA; 114308 MW; 156ABB6BA1B45A21 CRC64;
MKIYPYNELK TRFAEYAKPG EFNITSADTF RIIRLHYDEK QGCLFAFCNT NIKERVLQFY
FKVKLNLYSY KQCYDKHIFP SCRNKCISYT TFVAPGVEGN YLNKINVIKY ERNKAAPSDN
AACLDKFLHN VNRVHMQTPF VEGAYMRFKK TQRCQNNYVG GSTTRMFNLQ HFNEDFELVD
EMTLTSGIMP VLSCYDIETH SDGHNMSKAS VDCIMSIGFV VYKNDEYAKF CFMYHKLPTQ
IPETYDDDTY VVMFQNEIDM ITAFFDMIKI TNPDVILDFN GDVFDLPYIL GRLNKTKMLL
KRYDLPAAAP TTKLFINKLG NKVDTYYFNY YIHIDLYKFF SSDSNQHKVE NFQLNTISSY
YLGENKIDLP WTEMVKMYNT RRLDVIAKYN VQDCMLPIKL FVKLKMADSV YSQCILHRLC
TDDVICNISH LISVACFYAA ITNTRINEST GKEEPDPYFF NKNDLSIISG QFKADKAAAG
ISNLKRKLIP LKNIPKDAIN LGPANQTVKY KGGKVLKPRA GIYKNAFSLD FNSLYLTIMI
AICACLSNLI LCEDGNVYLN HNSRAIVVKL LLKLLSERCK FKKNRDNQSE SAFLYDLYDQ
KQNSVKRTAN SIYGYYGIFY KVLANYITRV GRNQLRLAIS LIEGLSNDPE ILEKFNLGSI
TFKVVYGDTD STFVLPTFNY NEISNETDTL KQICTHVETR VNNSFTDGYK MAFENLMKVL
ILLKKKKYCY LNSENKIVYK GWLVKKDMPV FMRIAFRTAV EQILRHLDMD KCLQSLQTSF
YEYYDEFAKS KSLTDYSFSM TYNDNPGKKR KSTDDNEGPS PKRRVITVAR HCREILVNKG
TDFVPGNGDR IPYLLIDIEG KVTEKAYPLR LFDPVKMRIS WIKHMGILCT FMNELLEIFG
DEQKDKIAKC FTAIMQKYMQ NQLYDRKEPV LVKINQKKCS VKRKRDDDDD NDDDDDDDCD
SSDSENDTQC ANNTYKFCLY KMKK
