ID   DPOL_ORFN2              Reviewed;        1008 AA.
AC   Q84173;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   29-SEP-2021, entry version 81.
DE   RecName: Full=DNA polymerase;
DE            EC=2.7.7.7;
GN   Name=POL;
OS   Orf virus (strain NZ2) (OV NZ-2).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Parapoxvirus.
OX   NCBI_TaxID=10259;
OH   NCBI_TaxID=9925; Capra hircus (Goat).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9940; Ovis aries (Sheep).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8758000; DOI=10.1099/0022-1317-77-7-1563;
RA   Mercer A.A., Green G., Sullivan J.T., Robinson A.J., Drillien R.;
RT   "Location, DNA sequence and transcriptional analysis of the DNA polymerase
RT   gene of orf virus.";
RL   J. Gen. Virol. 77:1563-1568(1996).
CC   -!- FUNCTION: Catalyzes DNA synthesis. Acquires processivity by associating
CC       with a heterodimeric processivity factor comprised of the viral A20 and
CC       D4 proteins, thereby forming the DNA polymerase holoenzyme. Displays
CC       3'- to 5' exonuclease activity. Might participate in viral DNA
CC       recombination. Does not perform translesion synthesis across an abasic
CC       site (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBUNIT: Interacts with A20. Component of the Uracil-DNA
CC       glycosylase(UDG)-A20-polymerase complex; A20 and UDG form a
CC       heterodimeric processivity factor that associates with E9 to form the
CC       processive polymerase holoenzyme (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
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DR   EMBL; U49979; AAB19092.1; -; Genomic_DNA.
DR   SMR; Q84173; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.90.1600.10; -; 2.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR   InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR   InterPro; IPR013617; DNA-dir_DNA_pol_B_vir_insert.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR013660; DNApol_B_exo_N.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF00136; DNA_pol_B; 1.
DR   Pfam; PF08408; DNA_pol_B_3; 1.
DR   Pfam; PF03104; DNA_pol_B_exo1; 1.
DR   Pfam; PF08452; DNAP_B_exo_N; 1.
DR   PRINTS; PR00106; DNAPOLB.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase;
KW   Nucleotidyltransferase; Transferase; Viral DNA replication.
FT   CHAIN           1..1008
FT                   /note="DNA polymerase"
FT                   /id="PRO_0000046536"
SQ   SEQUENCE   1008 AA;  113955 MW;  3C9A0450DA1990D1 CRC64;
     MELKCLNWFE NRGNDSRFLF LKARRADNAV VYLRFVQHFY YVVRADAVAD IAQPLAWTRA
     LGPMSVVSID EIVARSAKIP ERQRSEIELC LVASERKLAP PEVFMSDFLN VSWFFVAHDI
     DPDGCYRVDP ALLRDLGSNC FHCDDPGACF AEKIPRFNVT RSGLFLDIEC HFEKKFPSVF
     KNAVSHISFC VVDKDGAERR FTLTNSDMLS DADLEEAARR EIPVCLDPAD VKFDAEVTLC
     PEVTLLRVAK RLLEMPLDFV VTFNGHNFDL RYLDSRLSLL TGEHIRFRLP DGTETVNFCV
     YERTKSSHKG VGGVSSTTFH INNNNGTIYF DLYAFIQRTE KLDSYKLDAI SKNAFHCTAV
     VEDARPDAVR FRGDRSTDAD GNAAVFARVL STGNYVTVDE RVCRVLHKRV DEDGFTVDLA
     DPAARAPGDR VTLAFGKDDV SLADMYANYS LDVCLDMARY CLHDACLCLY LWSHYGVETK
     IAAAASTYLL PQSVVFEYRA STCIKGPLMK LLLENRTVMV RADTKSKFFY EGGRVMAPKQ
     KMHNKHVLIF DYNSLYPNVC IYANLSPETL VGVVVSDNRL DAEVAAVDVR RMFPAPRYIA
     VPCEPRSPEL VAEVAIFDRE AKGIIPMLLR SFLDARAKYK KLMKAAETAV DREIYNSMQY
     TYKITANSVY GLMGFRNSAL FSYASAKSCT AIGRTMIAYL ERTLDGASVC GTRLSLAAAP
     DNPLLRDEAF AGRAAELEID AAVAGERTER VGFRSVYGDT DSVFLEVGAS DIAFSRRVGR
     CLERVINEHV LFANFKVEFE AVYCNLIMQS KKKYTTIKFA VSDGGGSERV SKGTSETRRD
     VAPFHKLMIR KYKDMLCRAL AEEGSGNVGV EILRSLEDEL TFEFEARSMP LDWFLLSRTH
     HKNFKSPDNP NVALVTRYNA ANAEAIEIGE RYFFAYVCEE GPWRRRIANV KSYERVVDKT
     FRLDKNERIM YEVYFKRLCT EIVNLLDNKA MCTLFFERLF GSKPVFTG