ID   DPOL_PBCVN              Reviewed;         913 AA.
AC   P30320;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   29-SEP-2021, entry version 100.
DE   RecName: Full=DNA polymerase;
DE            EC=2.7.7.7;
GN   Name=DPO;
OS   Paramecium bursaria Chlorella virus NY2A (PBCV-NY2A).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes;
OC   Algavirales; Phycodnaviridae; Chlorovirus.
OX   NCBI_TaxID=46021;
OH   NCBI_TaxID=114055; Chlorella.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1585643; DOI=10.1016/0042-6822(92)90527-v;
RA   Grabherr R., Strasser P., van Etten J.L.;
RT   "The DNA polymerase gene from chlorella viruses PBCV-1 and NY-2A contains
RT   an intron with nuclear splicing sequences.";
RL   Virology 188:721-731(1992).
CC   -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC       potentially exhibits 3' to 5' exonuclease activity.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
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DR   EMBL; M86837; AAA88827.1; -; Genomic_DNA.
DR   SMR; P30320; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.132.60; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.90.1600.10; -; 1.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR   InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR042087; DNA_pol_B_thumb.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF00136; DNA_pol_B; 1.
DR   Pfam; PF03104; DNA_pol_B_exo1; 1.
DR   PRINTS; PR00106; DNAPOLB.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase; Early protein;
KW   Exonuclease; Hydrolase; Multifunctional enzyme; Nuclease;
KW   Nucleotidyltransferase; Transferase; Viral DNA replication.
FT   CHAIN           1..913
FT                   /note="DNA polymerase"
FT                   /id="PRO_0000046538"
FT   REGION          182..401
FT                   /note="Contains conserved residues essential for 3' -> 5'
FT                   exonuclease activities"
SQ   SEQUENCE   913 AA;  104956 MW;  7E656423123B02B5 CRC64;
     MTELTFFPTD WRSEDVEPDK GEPYFRINIF GKTMDGKTVC VRAKFTPFFL LETPESWSAA
     RTNLFITETA MKYDAIRPSC LPTKRKNMWG YDGGKMRPMV QFVFKTLSQM RKAKYRLKNE
     YQIYESSVDP IIRIFHLRNI NPADWMHVSK AFPVETRISN SDIEVETSFQ HLGPSDLKEV
     PPLIIASWDI ETYSKDRKFP LAENPADYCI QIATTFQKYG EPEPYRRVVV CYKQTASVEG
     VEIISCAEEA DVMNTWMTIL QDEITDVSIG YNLWQYDLRY IHGRSMMCVD DITGEDNVRL
     KNLGRLLVGG GEVIERDLSS NAFGQNKFFL LDMPGVMQID LLQWFRKNRN LESYSLNNVS
     KLYLGDQKND LPAMQIFEKF EGGADDRAII AAYARKDTDL PLKLLKKMAI LEDITEMANA
     VKVPVDYINF RGQQVRAFSC LVGKARQMNY AIPDDKMWTV DGKYEGATVL DAKKGAYFTS
     IAALDFASLY PSIIRAHNMS PETLVMDKRF ENLPGIEYYE IETGLGTFKY PQKNDETGEG
     QGVVPALLDD LAKFRKQAKK HMAEAKKNDD EFREALYDAQ QRSYKIVMNS VYGFLGASRG
     FIPCVPIAAS VTATGRKMIE HTAKRVTELL PGSEVIYGDT DSVMIRMKLP DDKIHDMDEQ
     FKMAKWLAGE ITKDFKAPND LEFEKIYYPY ILYSKKRYAA IKFEDPDEKG KVDVKGLALV
     RRDFSPITRE ILKESLDTIL FKKDTPTAVT ETVECIRKVL DNEYPMEKFT MSKTLKTGYK
     NECQPHLHVS NKIFERTGFP VPSGARVPFV YIEDKKNLDT KQSFRAEDPT FAQENDLIVD
     RLFYIEHQLM KPICSLFEPL LDDPETEIFG HPLIKGKIDE LKSTFKADLR DAKRTKKNIA
     NNQREITSFF KKK