ID   DPOL_PYRGL              Reviewed;         775 AA.
AC   Q9HH06; Q9P9K4;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=DNA polymerase;
DE            EC=2.7.7.7;
GN   Name=pol;
OS   Pyrococcus glycovorans.
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=74610;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AL585, and AL646;
RA   Querellou J.J.E., Cambon M.A., Lesongeur F., Forterre P., Barbier G.;
RT   "Thermococcales taxonomy and phylogeny based on the comparative use of 16S
RT   rDNA, 16S-23S rDNA intergenic spacer and family B DNA polymerase genes.";
RL   Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC       exhibits 3' to 5' exonuclease activity. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AJ004834; CAB81809.1; -; Genomic_DNA.
DR   EMBL; AJ250335; CAC12849.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9HH06; -.
DR   SMR; Q9HH06; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.132.60; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.90.1600.10; -; 1.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR   InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR042087; DNA_pol_B_thumb.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF00136; DNA_pol_B; 1.
DR   Pfam; PF03104; DNA_pol_B_exo1; 2.
DR   PRINTS; PR00106; DNAPOLB.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; DNA-directed DNA polymerase; Endonuclease;
KW   Hydrolase; Nuclease; Nucleotidyltransferase; Transferase.
FT   CHAIN           1..775
FT                   /note="DNA polymerase"
FT                   /id="PRO_0000278147"
FT   VARIANT         430
FT                   /note="R -> M (in strain: AL585)"
FT   VARIANT         467
FT                   /note="K -> R (in strain: AL585)"
FT   VARIANT         754
FT                   /note="G -> E (in strain: AL585)"
SQ   SEQUENCE   775 AA;  90418 MW;  633F8392EDBEEC73 CRC64;
     MILDADYITE DGKPIIRIFK KENGEFKVEY DRNFRPYIYA LLKDDSQIDE VKKITAERHG
     KIVRIVDVEK VKKKFLGRPI EVWKLYFEHP QDVPAIRDKI REHPAVVDIF EYDIPFAKRY
     LIDKGLIPME GDEELKLLAF DIETLYHEGE EFAKGPIIMI SYADEEGAKV ITWKKVDLPY
     VEVVSSEREM IKRFLKVIRE KDPDVIITYN GDSFDLPYLV KRAEKLGIKL PLGRDGSEPK
     MQRLGDMTAV EIKGRIHFDL YHVIRRTINL PTYTLEAVYE AIFGKPKEKV YAHEIAEAWE
     TGKGLERVAK YSMEDAKVTY ELGREFFPME AQLSRLVGQP LWDVSRSSTG NLVEWYLLRK
     AYERNELAPN KPDEREYERR LRESYAGGYV KEPEKGLWEG LVSLDFRSLY PSIIITHNVS
     PDTLNREGCR EYDVAPEVKH KFCKDFPGFI PSLLKRLLDE RQEIKRKMKA SKDPIEKKML
     DYRQRAIKIL ANSYYGYYGY AKARWYCKEC AESVTAWGRE YIEFVRKELE EKFGFKVLYI
     DTDGLYATIP GAKPEEIKRK ALEFVEYINA KLPGLLELEY EGFYVRGFFV TKKKYALIDE
     EGKIITRGLE IVRRDWSEIA KETQAKVLEA ILKHGNVEEA VKIVKEVTEK LSKYEIPPEK
     LVIYEQITRP LHEYKAIGPH VAVAKRLAAR GVKVRPGMVI GYIVLRGDGP ISKRAILAEE
     FDPRKHKYDA EYYIENQVLP AVLRILEAFG YRKGDLRWQK TKQTGLTAWL NVKKK