ID   DPOL_PYRHO              Reviewed;        1235 AA.
AC   O59610;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 139.
DE   RecName: Full=DNA polymerase;
DE            EC=2.7.7.7;
DE   Contains:
DE     RecName: Full=Pho pol intein;
DE     AltName: Full=Pho Pol I intein;
GN   Name=pol; OrderedLocusNames=PH1947; ORFNames=PHBT047;
OS   Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS   100139 / OT-3).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=70601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX   PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA   Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA   Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA   Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA   Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- PTM: This protein undergoes a protein self splicing that involves a
CC       post-translational excision of the intervening region (intein) followed
CC       by peptide ligation. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
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DR   EMBL; BA000001; BAA31074.1; -; Genomic_DNA.
DR   PIR; C71210; C71210.
DR   RefSeq; WP_010886013.1; NC_000961.1.
DR   AlphaFoldDB; O59610; -.
DR   SMR; O59610; -.
DR   STRING; 70601.3258391; -.
DR   EnsemblBacteria; BAA31074; BAA31074; BAA31074.
DR   GeneID; 1442795; -.
DR   KEGG; pho:PH1947; -.
DR   eggNOG; arCOG00328; Archaea.
DR   eggNOG; arCOG03145; Archaea.
DR   OMA; YAHNSYY; -.
DR   OrthoDB; 35869at2157; -.
DR   BRENDA; 2.7.7.7; 5244.
DR   Proteomes; UP000000752; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR   Gene3D; 1.10.132.60; -; 1.
DR   Gene3D; 3.10.28.10; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.90.1600.10; -; 2.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR   InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR042087; DNA_pol_B_thumb.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR003586; Hint_dom_C.
DR   InterPro; IPR003587; Hint_dom_N.
DR   InterPro; IPR036844; Hint_dom_sf.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR006142; INTEIN.
DR   InterPro; IPR030934; Intein_C.
DR   InterPro; IPR004042; Intein_endonuc.
DR   InterPro; IPR006141; Intein_N.
DR   InterPro; IPR041005; PI-TkoII_IV.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF00136; DNA_pol_B; 2.
DR   Pfam; PF03104; DNA_pol_B_exo1; 2.
DR   Pfam; PF18714; PI-TkoII_IV; 1.
DR   PRINTS; PR00106; DNAPOLB.
DR   PRINTS; PR00379; INTEIN.
DR   SMART; SM00305; HintC; 1.
DR   SMART; SM00306; HintN; 1.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF51294; SSF51294; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF55608; SSF55608; 1.
DR   SUPFAM; SSF56672; SSF56672; 2.
DR   TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR   TIGRFAMs; TIGR01445; intein_Nterm; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
DR   PROSITE; PS50818; INTEIN_C_TER; 1.
DR   PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR   PROSITE; PS50817; INTEIN_N_TER; 1.
PE   3: Inferred from homology;
KW   Autocatalytic cleavage; DNA replication; DNA-binding;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Protein splicing;
KW   Transferase.
FT   CHAIN           1..492
FT                   /note="DNA polymerase, 1st part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000007322"
FT   CHAIN           493..952
FT                   /note="Pho pol intein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000007323"
FT   CHAIN           953..1235
FT                   /note="DNA polymerase, 2nd part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000007324"
FT   DOMAIN          773..887
FT                   /note="DOD-type homing endonuclease"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00273"
SQ   SEQUENCE   1235 AA;  143087 MW;  73CC7AA14873CCE4 CRC64;
     MILDADYITE DGKPIIRIFK KENGEFKVEY DRNFRPYIYA LLRDDSAIDE IKKITAQRHG
     KVVRIVETEK IQRKFLGRPI EVWKLYLEHP QDVPAIRDKI REHPAVVDIF EYDIPFAKRY
     LIDKGLTPME GNEKLTFLAV DIETLYHEGE EFGKGPVIMI SYADEEGAKV ITWKKIDLPY
     VEVVSSEREM IKRLIRVIKE KDPDVIITYN GDNFDFPYLL KRAEKLGIKL LLGRDNSEPK
     MQKMGDSLAV EIKGRIHFDL FPVIRRTINL PTYTLEAVYE AIFGKPKEKV YADEIAKAWE
     TGEGLERVAK YSMEDAKVTY ELGREFFPME AQLARLVGQP VWDVSRSSTG NLVEWFLLRK
     AYERNELAPN KPDEKEYERR LRESYEGGYV KEPEKGLWEG IVSLDFRSLY PSIIITHNVS
     PDTLNREGCE EYDVAPKVGH RFCKDFPGFI PSLLGQLLEE RQKIKKRMKE SKDPVEKKLL
     DYRQRAIKIL ANSILPDEWL PIVENEKVRF VKIGDFIDRE IEENAERVKR DGETEILEVK
     DLKALSFNRE TKKSELKKVK ALIRHRYSGK VYSIKLKSGR RIKITSGHSL FSVKNGKLVK
     VRGDELKPGD LVVVPGRLKL PESKQVLNLV ELLLKLPEEE TSNIVMMIPV KGRKNFFKGM
     LKTLYWIFGE GERPRTAGRY LKHLERLGYV KLKRRGCEVL DWESLKRYRK LYETLIKNLK
     YNGNSRAYMV EFNSLRDVVS LMPIEELKEW IIGEPRGPKI GTFIDVDDSF AKLLGYYISS
     GDVEKDRVKF HSKDQNVLED IAKLAEKLFG KVRRGRGYIE VSGKISHAIF RVLAEGKRIP
     EFIFTSPMDI KVAFLKGLNG NAEELTFSTK SELLVNQLIL LLNSIGVSDI KIEHEKGVYR
     VYINKKESSN GDIVLDSVES IEVEKYEGYV YDLSVEDNEN FLVGFGLLYA HNSYYGYYGY
     AKARWYCKEC AESVTAWGRQ YIDLVRRELE ARGFKVLYID TDGLYATIPG VKDWEEVKRR
     ALEFVDYINS KLPGVLELEY EGFYARGFFV TKKKYALIDE EGKIVTRGLE IVRRDWSEIA
     KETQARVLEA ILKHGNVEEA VKIVKDVTEK LTNYEVPPEK LVIYEQITRP INEYKAIGPH
     VAVAKRLMAR GIKVKPGMVI GYIVLRGDGP ISKRAISIEE FDPRKHKYDA EYYIENQVLP
     AVERILKAFG YKREDLRWQK TKQVGLGAWI KVKKS