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DPOL_THES9
ID   DPOL_THES9              Reviewed;         775 AA.
AC   Q56366;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=DNA polymerase;
DE            EC=2.7.7.7;
GN   Name=pol; Synonyms=polA;
OS   Thermococcus sp. (strain 9oN-7).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Thermococcus; unclassified Thermococcus.
OX   NCBI_TaxID=103799;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8643567; DOI=10.1073/pnas.93.11.5281;
RA   Southworth M.W., Kong H., Kucera R.B., Ware J., Jannasch H.W., Perler F.B.;
RT   "Cloning of thermostable DNA polymerases from hyperthermophilic marine
RT   Archaea with emphasis on Thermococcus sp. 9oN-7 and mutations affecting 3'-
RT   5' exonuclease activity.";
RL   Proc. Natl. Acad. Sci. U.S.A. 93:5281-5285(1996).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}.
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DR   EMBL; U47108; AAA88769.1; -; Genomic_DNA.
DR   PIR; S67920; S67920.
DR   PDB; 1QHT; X-ray; 2.10 A; A=1-775.
DR   PDB; 4K8X; X-ray; 2.28 A; A=1-775.
DR   PDB; 5OMQ; X-ray; 2.20 A; A=1-775.
DR   PDB; 5OMV; X-ray; 2.00 A; A=1-775.
DR   PDB; 6IS7; X-ray; 2.80 A; A/B=1-775.
DR   PDB; 6ISF; X-ray; 2.80 A; A/B=1-775.
DR   PDB; 6ISG; X-ray; 3.40 A; A=1-775.
DR   PDB; 6ISH; X-ray; 3.30 A; A=1-775.
DR   PDB; 6ISI; X-ray; 3.20 A; A=1-775.
DR   PDBsum; 1QHT; -.
DR   PDBsum; 4K8X; -.
DR   PDBsum; 5OMQ; -.
DR   PDBsum; 5OMV; -.
DR   PDBsum; 6IS7; -.
DR   PDBsum; 6ISF; -.
DR   PDBsum; 6ISG; -.
DR   PDBsum; 6ISH; -.
DR   PDBsum; 6ISI; -.
DR   AlphaFoldDB; Q56366; -.
DR   SMR; Q56366; -.
DR   EvolutionaryTrace; Q56366; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.132.60; -; 1.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.90.1600.10; -; 1.
DR   InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR   InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR   InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR   InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR042087; DNA_pol_B_thumb.
DR   InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF00136; DNA_pol_B; 1.
DR   Pfam; PF03104; DNA_pol_B_exo1; 2.
DR   PRINTS; PR00106; DNAPOLB.
DR   SMART; SM00486; POLBc; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA replication; DNA-binding; DNA-directed DNA polymerase;
KW   Nucleotidyltransferase; Transferase.
FT   CHAIN           1..775
FT                   /note="DNA polymerase"
FT                   /id="PRO_0000046489"
FT   STRAND          2..10
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          13..22
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          25..31
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          37..43
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           45..47
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           48..51
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          55..58
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          61..64
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          67..75
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          78..86
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           92..102
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          106..111
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           116..123
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          137..144
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          157..163
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          168..174
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          181..183
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           187..201
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          204..210
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   TURN            211..214
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           215..226
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          233..236
FT                   /evidence="ECO:0007829|PDB:6IS7"
FT   STRAND          240..244
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          247..251
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          255..259
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           260..267
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           275..283
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           292..301
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          302..304
FT                   /evidence="ECO:0007829|PDB:6ISH"
FT   HELIX           305..337
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           341..344
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           349..364
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           374..379
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   TURN            380..382
FT                   /evidence="ECO:0007829|PDB:1QHT"
FT   STRAND          395..405
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           408..416
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   TURN            420..422
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          431..433
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   TURN            435..437
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          440..442
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           448..469
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           473..497
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           507..531
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          535..540
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          543..547
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           553..567
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           568..570
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          577..590
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          593..598
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          603..608
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           609..611
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          613..615
FT                   /evidence="ECO:0007829|PDB:6ISF"
FT   HELIX           617..633
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           636..651
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           657..660
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          662..665
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           670..672
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           678..688
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          698..705
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           710..712
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   STRAND          714..716
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           717..719
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   TURN            722..724
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           729..734
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           737..745
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           746..748
FT                   /evidence="ECO:0007829|PDB:5OMV"
FT   HELIX           752..754
FT                   /evidence="ECO:0007829|PDB:5OMV"
SQ   SEQUENCE   775 AA;  89781 MW;  71525D63C0C9CD9D CRC64;
     MILDTDYITE NGKPVIRVFK KENGEFKIEY DRTFEPYFYA LLKDDSAIED VKKVTAKRHG
     TVVKVKRAEK VQKKFLGRPI EVWKLYFNHP QDVPAIRDRI RAHPAVVDIY EYDIPFAKRY
     LIDKGLIPME GDEELTMLAF DIETLYHEGE EFGTGPILMI SYADGSEARV ITWKKIDLPY
     VDVVSTEKEM IKRFLRVVRE KDPDVLITYN GDNFDFAYLK KRCEELGIKF TLGRDGSEPK
     IQRMGDRFAV EVKGRIHFDL YPVIRRTINL PTYTLEAVYE AVFGKPKEKV YAEEIAQAWE
     SGEGLERVAR YSMEDAKVTY ELGREFFPME AQLSRLIGQS LWDVSRSSTG NLVEWFLLRK
     AYKRNELAPN KPDERELARR RGGYAGGYVK EPERGLWDNI VYLDFRSLYP SIIITHNVSP
     DTLNREGCKE YDVAPEVGHK FCKDFPGFIP SLLGDLLEER QKIKRKMKAT VDPLEKKLLD
     YRQRAIKILA NSFYGYYGYA KARWYCKECA ESVTAWGREY IEMVIRELEE KFGFKVLYAD
     TDGLHATIPG ADAETVKKKA KEFLKYINPK LPGLLELEYE GFYVRGFFVT KKKYAVIDEE
     GKITTRGLEI VRRDWSEIAK ETQARVLEAI LKHGDVEEAV RIVKEVTEKL SKYEVPPEKL
     VIHEQITRDL RDYKATGPHV AVAKRLAARG VKIRPGTVIS YIVLKGSGRI GDRAIPADEF
     DPTKHRYDAE YYIENQVLPA VERILKAFGY RKEDLRYQKT KQVGLGAWLK VKGKK
 
 
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